YSTIC echinococcosis (CE) is a cyclozoonotic parasitic infection of worldwide distribution particularly in in developing countries and rural communities. Serological and immunological assays are useful for both for the initial diagnosis of infection in humans and animals as well as improving the quality of disease management. This study compares and assesses the efficacy of various hydatid cyst antigens for the accurate immunological diagnosis of CE in both humans and camels. Outer layer (Ol.C) germinal layer (Gl.C) fluid (Fl.C) and protoscolices (Ps.C) antigens prepared from camel hydatid cyst. In addition, fluid (Fl.H) and protoscolices (Ps.H) prepared from human hydatid cyst aspirate. All six-antigen types investigated as immunodiagnostic tools in human and camel hydatidosis using ELISA, SDS-PAGE and western blot assays. ELISA showed higher binding reactivity by protoscolices antigens (Ps.C and Ps.H) followed by fluid antigens (Fl.C and Fl.H), while outer and germinal layer antigens (Ol.C and Gl.C) give the lower activates. Ps.C antigen exhibiting higher sensitivity (100%), than Ps.H antigen (84.6 and 85.3%) against both positive camels and human sera. The sero-prevalence of Cystic echinococcosis was 59.78% and 43.65% in examined camel and human sera, respectively using the diagnostic Ps.C antigen. According to SDS-PAGE, all six hydatid cyst antigens demonstrated significant electrophoretic similarity, particularly in three bands of 42, 36, and 22 kDa, Using immunoblotting techniques, all separated antigens identified a sharing in two common bands with the same molecular weights of 42 a and 22 kDa. The obtained results concluded that camel and human hydatid cyst protoscolices antigens are introduced in this investigation as an effective diagnostic antigen and new immune-reactive fractions of 42 and 22 kDa for the diagnosis of hydatidosis in humans and camels.