Diagnostic Exercise: Tyzzer's Disease, Distemper, and Coccidiosis in a Pup

Headley, Selwyn Arlington; Shirota, Kinji; Baba, Tomoshige; Ikeda, Teruo; Sukura, Antti

doi:10.1354/vp.46-1-151

Cited by 21 publications

(24 citation statements)

References 4 publications

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“…g The intensity and distribution of immunoreactivity of the panel of antibodies were subjectively evaluated and combined into grades of 0, absent; +, discrete; ++, moderate; and +++, marked. Tissue sections from a previous case of distemper 10 and toxoplasmosis (Headley, unpublished data) were used as positive controls. For negative controls, phosphate buffered saline solution was substituted for the primary antibody.…”

Section: Clinical History and Necropsymentioning

confidence: 99%

Molecular Detection of Canine Distemper Virus and the Immunohistochemical Characterization of the Neurologic Lesions in Naturally Occurring Old Dog Encephalitis

Headley

Amude

Alfieri³

et al. 2009

J VET Diagn Invest

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Abstract. The current article describes a spontaneous case of old dog encephalitis (ODE) in a 7-year-old, intact, female Miniature Schnauzer dog from Londrina, Paraná , southern Brazil. Unlike conventional distemper encephalomyelitis, ODE is a poorly understood and extremely rare manifestation of Canine distemper virus (CDV) infection. The dog was presented with progressive clinical manifestations consistent with cerebral dysfunction. Briefly, histopathologic lesions were restricted to the forebrain and included chronic multifocal lymphoplasmacytic encephalitis with extensive perivascular cuffing, astrocytosis, and intranuclear inclusions within astrocytes and giant cells, with both intracytoplasmic and intranuclear inclusions. Immunohistochemistry (IHC) was used to identify the antigens of the nucleoprotein (NP) of CDV and to detect cluster of differentiation (CD)3, CD79a, macrophage (MAC) 387, glial fibrillary acidic protein, and vimentin to characterize the neuroparenchymal lesions. By IHC, CDV NP was demonstrated predominantly within neurons and astrocytes. Cells that formed perivascular cuffs and some astrocyte-like cells reacted intensely to vimentin. Reverse transcription polymerase chain reaction assay from brain sections further confirmed a role for CDV in this disease by the amplification and partial sequence analysis of the NP gene. These findings confirmed simultaneous detection of CDV in ODE by IHC and molecular assays. In addition, results of the current study could contribute to the neuropathologic characterization of this rare manifestation of CDV.

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Section: Clinical History and Necropsymentioning

confidence: 99%

Molecular Detection of Canine Distemper Virus and the Immunohistochemical Characterization of the Neurologic Lesions in Naturally Occurring Old Dog Encephalitis

Headley

Amude

Alfieri³

et al. 2009

J VET Diagn Invest

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“…It must be highlighted that coinfections of CaKV with other viral agents were identified in several tissues/organs, including the cerebrum, lung, and the tonsils (Table 1); and there was concomitant parvovirus enteritis and giardiasis. Mixed infections in dogs have been previously related with descriptions of simultaneous viral, bacterial, and parasitic agents [10,11], as well as mixed infections with multiple viral agents [12].…”

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confidence: 99%

Extra-intestinal detection of canine kobuvirus in a puppy from Southern Brazil

et al. 2016

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This study presents the pathological, immunohistochemical, and molecular findings associated with the extra-intestinal detection of canine kobuvirus (CaKV) in a 5-month-old Chihuahua puppy, that had a clinical history of bloody-tinged feces. Principal pathological findings were interstitial pneumonia, necrotizing bronchitis, and parvovirus-induced enteritis. Molecular diagnostic methods identified CaKV within the cerebellum, cerebrum, lung, tonsil, and liver. CaKV and rotavirus were not identified within the feces and intestine. Immunohistochemistry (IHC) assays detected antigens of CDV and CAdV-1 in the lungs. These results confirmed the extra-intestinal detection of CaKV in this puppy and represent the first extra-intestinal detection of CaKV in a dog.

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“…Clostridium piliforme usually causes sporadic disease, which has been observed in many species of domestic, wild, and laboratory animals, including rodents, lagomorphs, dogs, cats, horses, birds, and primates. Other lesions commonly reported for animals with Tyzzer' s disease, among which are paintbrush hemorrhages in the cecum, mucosal necrosis and edema of the intestine, and myocardial necrosis (particularly near the apex), 5,8 were not present in this oryx. Carrier animals are a possible source of infective spores, with transmission likely occurring through ingestion of fecescontaminated soil or feed.…”

Section: Commentsmentioning

confidence: 77%