Objective: To evaluate the condylar changes through cone-beam computed tomography (CBCT) images in patients treated with Twin-Block functional appliance. Materials and Methods: In this retrospective study, CBCT images of 30 patients who were treated with the Twin-Block appliance were used. Mandible was segmented and pretreatment and posttreatment (T0 and T1) condylar volume was compared. The angle between sella-nasion-Point A (SNA), angle between sella-nasion-Point B (SNB), angle between Point A-nasion-Point B (ANB), midfacial length (Co-A), mandibular length (Co-Gn), and the distances from right condylion to left condylion (Co R -Co L ) were also measured on three-dimensional images. Differences were analyzed with Wilcoxon signed rank tests, and Mann-Whitney U-tests were used to compare the scores of male and female participants. Significance was set at P , .05. Results: In this study, a decrease in SNA and ANB (P , .05 and P , .01, respectively) and an increase in SNB (P , .01) were found. Additionally, Co R -Co L , Co-Gn, and condylar volume increased at both the left and right sides (P , .01). However, increase at Co-A was not statistically significant (P . .05). Comparison of differences by sex was not statistically significant for all measurements (P . .05). Conclusion: Twin-Block appliance increases condylar volume, mandibular length, and intercondylar distance by stimulating growth of condyle in an upward and backward direction.