Diagnostic of Biomphalaria snails and Schistosoma mansoni: DNA obtained from traces of shell organic materials

Caldeira, Roberta Lima; Jannotti-Passos, Liana K.; Lira, Pollanah M; Carvalho, Omar S.

doi:10.1590/s0074-02762004000500007

Cited by 20 publications

(10 citation statements)

References 14 publications

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“…Most of the molecular investigations (Carvalho et al 2001, Caldeira et al 2004) confirm results from previous research that was based on mor- phological analyses, which were carried out by Paraense during a 50-year period of Biomphalaria taxonomic classification. Because of the molecular studies, specific molecular profiles were generated and standardized for Brazilian snail species by digesting the internal transcribed spacer region (ITS) of rDNAs with the restriction enzyme DdeI (Vidigal et al 2000a).…”

supporting

confidence: 77%

Preliminary studies investigating the occurrence of Biomphalaria cousini in Brazil

Caldeira

Teodoro

Gomes

et al. 2010

Mem. Inst. Oswaldo Cruz

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supporting

confidence: 77%

Preliminary studies investigating the occurrence of Biomphalaria cousini in Brazil

Caldeira

Teodoro

Gomes

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…Mitochondrial DNA extraction and amplification from mollusc shells has been reported in a variety of species, from fresh shells of freshwater pearl mussels ( Geist, Wunderlich & Kuehn, 2008 ) and Pacific oyster ( Wang et al, 2012 ), up to 50 year old snail shells ( Andree & López, 2013 ; Caldeira et al, 2004 ; Villanea, Parent & Kemp, 2016 ), 70 year old abalone shells ( Hawk, 2010 ) and even 125 year old oyster shells ( Barsh & Murphy, 2008 ). In fact, Der Sarkissian et al (2017) recently established that shell DNA extraction from a variety of marine mollusc species aged up to 7,000 years BP is possible and can be used to retrieve not only mollusc endogenous DNA, but also that of the natural microbiome, which could include pathogenic organisms, symbiotic to living molluscs.…”

Section: Discussionmentioning

confidence: 99%

DNA from mollusc shell: a valuable and underutilised substrate for genetic analyses

Ferreira

Ashby

Jeunen

et al. 2020

PeerJ

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Mollusc shells are an abundant resource that have been long used to predict the structures of ancient ecological communities, examine evolutionary processes, reconstruct paleoenvironmental conditions, track and predict responses to climatic change, and explore the movement of hominids across the globe. Despite the ubiquity of mollusc shell in many environments, it remains relatively unexplored as a substrate for molecular genetic analysis. Here we undertook a series of experiments using the New Zealand endemic greenshell mussel, Perna canaliculus, to explore the utility of fresh, aged, beach-cast and cooked mollusc shell for molecular genetic analyses. We find that reasonable quantities of DNA (0.002–21.48 ng/mg shell) can be derived from aged, beach-cast and cooked mussel shell and that this can routinely provide enough material to undertake PCR analyses of mitochondrial and nuclear gene fragments. Mitochondrial PCR amplification had an average success rate of 96.5% from shell tissue extracted thirteen months after the animal’s death. A success rate of 93.75% was obtained for cooked shells. Amplification of nuclear DNA (chitin synthase gene) was less successful (80% success from fresh shells, decreasing to 10% with time, and 75% from cooked shells). Our results demonstrate the promise of mollusc shell as a substrate for genetic analyses targeting both mitochondrial and nuclear genes.

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“…Patent infection rates in snails are determined by searching for cercariae coming out from snails by two routine examination methods; shedding method, which was induced by exposure of snails to artificial light (Webbe 1965), and crushing method, which was performed by squeezing the snails between two glass slides followed by microscopic examination (Chu and Dawood 1970). These methods are more or less accurate in detection of snail prepatent infection (detection of cercarial shedding is only possible 30 days after infection), timeconsuming, unsuitable for routine large-scale screening of snail populations (Caldeira et al 2004). Subsequently, detailed data on prepatent infection, which can constitute a significant proportion of infected snails populations and correspond to infection prevalence in human populations contacting the sites studied, have not been available (Abbasi et al 2010;Hamburger et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Molecular approach for detecting early prepatent Schistosoma mansoni infection in Biomphalaria alexandrina snail host

et al. 2014

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The present study aimed to evaluate a polymerase chain reaction (PCR) assay used for detection of Schistosoma mansoni infection in Biomphalaria alexandrina snails in early prepatent period and to compare between it and the ordinary detection methods (shedding and crushing). Biomphalaria alexandrina snails are best known for their role as intermediate hosts of S. mansoni. DNA was extracted from infected snails in addition to noninfected ''negative control'' (to optimized the efficiency of PCR reaction) and subjected to PCR using primers specific to a partial sequence of S. mansoni fructose-1,6-bus phosphate aldolase (SMALDO). SMALDO gene was detected in the infected laboratory snails with 70, 85, and 100 % positivity at the 1st, 3rd, and 7th day of infection, respectively. In contrast, the ordinary method was not sensitive enough in detection of early prepatent infection even after 7 days of infection which showed only 25 % positivity. By comparing the sensitivity of the three methods, it was found that the average sensitivity of shedding method compared to PCR was 23.8 % and the average sensitivity of crushing method compared to PCR was 46.4 % while the sensitivity of PCR was 100 %. We conclude that PCR is superior to the conventional methods and can detect positive cases that were negative when examined by shedding or crushing methods. This can help in detection of the areas and times of high transmission which in turn will be very beneficial in planning of the exact timing of the proper control strategy.

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Diagnostic of Biomphalaria snails and Schistosoma mansoni: DNA obtained from traces of shell organic materials

Cited by 20 publications

References 14 publications

Preliminary studies investigating the occurrence of Biomphalaria cousini in Brazil

Preliminary studies investigating the occurrence of Biomphalaria cousini in Brazil

DNA from mollusc shell: a valuable and underutilised substrate for genetic analyses

Molecular approach for detecting early prepatent Schistosoma mansoni infection in Biomphalaria alexandrina snail host

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