Diagnostic performance of direct wet mount microscopy in detecting intestinal helminths among pregnant women attending ante-natal care (ANC) in East Wollega, Oromia, Ethiopia

Mengist, Hylemariam Mihiretie; Demeke, Gebreselassie; Zewdie, Olifan; Belew, Adugna

doi:10.1186/s13104-018-3380-z

Cited by 27 publications

(20 citation statements)

References 16 publications

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“…Moreover, the presence of large stool debris materials in the DWMM may conceal the parasitic ova. The sensitivity of DWMM in this study (37%) is similar with other studies done in Ethiopia (25,27). This suggests that DWMM has resulted in around 63% false negative reports during hookworm diagnosis.…”

Section: Discussionsupporting

confidence: 89%

“…For instance, DWMM is solely used in almost all health care facilities in Ethiopia and other developing countries due to its low cost, and easy procedure. Nevertheless, it is undoubtedly known that the sensitivity of DWMM is poor (25,26). In spite of this, there are little studies that have evaluated the clinical sensitivity of DWMM compared to other microscopical techniques for the diagnosis of intestinal parasitosis including Hookworm infection.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Hookworm Diagnosis Techniques from Patients in Amhara Region, Ethiopia: Cross sectional study

Zeleke

Addisu

Tegegne

et al. 2020

Preprint

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Background: Inappropriate diagnosis could intimidate the prevention and control of Hookworm infection. This study was aimed at evaluating the performance of Hookworm diagnosis methods.Methods: An institution based cross sectional study was conducted in Amhara region, Ethiopia. The study subjects were selected conveniently. Sensitivity, specificity, predictive values, test accuracy and agreements of the different hookworm diagnosis methods, namely; Test Tube Flotation (TFT), MacMaster (MM), Formol Ether Concentration (FEC), Kato katz (KK), and Direct wet mount microscopy (DWMM) were calculated. Composite reference standard (CRS) was used as a gold standard method. Kappa (κ) test was used to measure the level of agreement between diagnosis tests. Moreover, t-test was used to compare the diagnostic performance of the diagnosis methods.Result: a total of 389 stool samples were collected from patients in the study. The overall prevalence of hookworm was 63.24%. Test Tube Flotation (TFT) was found to be the highest both in terms of sensitivity and diagnosis accuracy (100%). MacMaster (MM) was the second most sensitive test (68.7%), followed by FEC (44.3%) and KK (38.2%). On the other hand, DWMM had the lowest sensitivity (37.4%) and its diagnosis accuracy was also the least (60%). Only TFT had a perfect (Agreement=100%, kappa=1) with the CRS. The sensitivity of DWMM, KK, and FEC showed an increase in sensitivity as a function of increasing intensity of infection, but TFT and MM methods were not affected by the infection intensity. Conclusion: Hookworm is still a public health problem in the study area. TFT is by far more sensitive than MM, FEC, KK and DWMM techniques.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Evaluation of Hookworm Diagnosis Techniques from Patients in Amhara Region, Ethiopia: Cross sectional study

Zeleke

Addisu

Tegegne

et al. 2020

Preprint

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“…Direct wet mount of stool has a low sensitivity for detecting light helminthic infections [13]. In two studies, wet mount had detection rates of 18.8% vs. 24.7% for formalin-ether concentration (FEC) [14], and 48.9% vs. 63.1 and 93.7% for FEC and Kato Katz tests, respectively [15]. Nuchprayoon et al (2009) suggest that the simple direct smear is insufficiently sensitive to be used alone for stool parasite screening [16].…”

Section: Introductionmentioning

confidence: 99%

Modified Harada-Mori and simple wet mount to determine hookworm infections in Yo Island urban area, Songkhla, Southern Thailand

et al. 2019

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Background Hookworm was a previously dominant parasitic infection in Southern Thailand. The changing population to an aging society in Yo Island has never been investigated for intestinal parasites. This study aimed to estimate the prevalence of hookworm and intestinal parasitic infections on Yo Island, a small island in Songkhla Province of southern Thailand. Methods A cross-sectional study was conducted among volunteers aged 15 and above to give one stool sample that was screened by wet mount for intestinal parasites and the modified Harada-Mori culture (mHMFPC) which is adapted from HMFPC, using local plastic bag containers instead of test tubes for hookworm detection. Results Two hundred forty-seven volunteers (females = 160) gave one stool. The highest participation was in age group higher than 60 years. Most were Buddhism (89.1%), agriculturist (71.4%), non-education (87.9%), and income lower than 9000 baht (50.2%). The prevalence of intestinal parasites was 13/247 (5.3%) of which 6/247 (2.4%) were positive for hookworm species Necator americanus. One volunteer was coinfected with hookworm and Strongyloides stercoralis and another with Endolimax nana and Blastocystis hominis . The mHMFPC detected more positive stool samples than wet mount and wet mount: 5 vs. 2. Conclusions Parasite prevalence was low in this urban community of mostly low-income village dwellers. The mHMFPC appeared better at detecting hookworm but numbers were small. Combined techniques are suitable for field use.

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“…This study had concluded that a direct stool wet mount was not a sensitive method for detecting H . nana infection in particular and other intestinal helminths [7].…”

Section: Discussionmentioning

confidence: 99%

Hymenolepiasis in a Pregnant Woman: A Case Report of Hymenolepis nana Infection

Kandi

Koka

Bhoomigari

2019

Cureus

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Hymenolepiasis is an infection caused by Hymenolepis nana (H. nana) and H. diminuta (H. diminuta). Hymenolepiasis is prevalent throughout the world with human infections with H. nana being frequently reported in the literature as compared to H. diminuta. Hymenolepiasis is more frequent among children, and most human infections remain asymptomatic and selflimited. Symptoms including abdominal pain, diarrhea, and vomiting are frequently noted in the cases of heavy infections. We report a case of hymenolepiasis caused by H. nana in a pregnant woman.

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Diagnostic performance of direct wet mount microscopy in detecting intestinal helminths among pregnant women attending ante-natal care (ANC) in East Wollega, Oromia, Ethiopia

Cited by 27 publications

References 16 publications

Evaluation of Hookworm Diagnosis Techniques from Patients in Amhara Region, Ethiopia: Cross sectional study

Evaluation of Hookworm Diagnosis Techniques from Patients in Amhara Region, Ethiopia: Cross sectional study

Modified Harada-Mori and simple wet mount to determine hookworm infections in Yo Island urban area, Songkhla, Southern Thailand

Hymenolepiasis in a Pregnant Woman: A Case Report of Hymenolepis nana Infection

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