Diagnostic performance of PCA3 and hK2 in combination with serum PSA for prostate cancer

Abstract

Objectives:The prostate cancer gene 3 (PCA3), human kallikrein 2, and miRNA-141 are promising prostate cancer (Pca) specific biomarkers. Our aim was to evaluate the detection of PCA3, human glandular kallikrein 2 (hk2), and miRNA-141 mRNA in peripheral blood of patients received prostate biopsy. What's more, we want to detect the value of combination of PSA (prostate specific antigen) in the early diagnosis of PCa.Materials and methods:Hundred patients were divided into 2 groups according to the results of pathologic diagnosis. Quantitative real-time PCR (qRT-PCR) was used to evaluate the mRNA of PCA3, hk2, and miRNA-141 in peripheral blood. At the same time, analyze those clinical outcomes used in the patients. We compared these different outcomes to evaluate the value of new molecular markers.Results:The level of mRNA of PCA3, hK2, and miR-141 in Pca group were significantly higher than that in BPH. PSA had the highest sensitivity in predicting Pca diagnosis (76.7%); PCA3 had the highest specificity (82.5%). And the combination of PCA3, PSA, and hK2 improved area under the curve (AUC)-receiver operating characteristic (ROC) curve largely, especially those with PSA 4-10ng/mL.Conclusions:PCA3, hK2, and miRNA-141 were biomarkers of Pca with potential clinical application value, especially in patients with PSA gray area. Combining PCA3, PSA, and hK2 performed better than individual biomarkers alone in predicting Pca.

The long non-coding RNA PCA3: an update of its functions and clinical applications as a biomarker in prostate cancer

“…In an attempt to improve PCa diagnosis, some studies have demonstrated better results of PCA3 in association with PSA [64, 65], in addition to prostate-specific G-protein coupled receptor (PSGR) [66], a biomarker previously described to be overexpressed in PCa tissue [67]. Other authors found that the combination of PCA3 , PSA, and human kallikrein 2 (hK2) largely improved area under the curve (AUC)-receiver operating characteristic (ROC), especially those patients presenting PSA 4-10 ng/mL [64]. PCa was quantitatively detected through overexpressed PCA3 and PSA genes, in urine sediments of men with PCa or BPH, after prostatic massage.…”

FOXE1 represses cell proliferation and Warburg effect by inhibiting HK2 in colorectal cancer

“…*P < 0.05 produce glucose-6-phosphate [31]. Previous studies have confirmed that the expression of HK2 is significantly upregulated in many cancers and its high expression in cancers is associated with poor prognosis [30,[32][33][34][35]. Administration of HK2 inhibitor, 2-DG, can induce cancer cell death by abrogating intracellular glycolysis.…”

Markers of Toxicity and Response to Radiation Therapy in Patients With Prostate Cancer

“… 95 Zidan et al 96 reported that plasma levels of miRNAs -21, -125b, -126, -141, -143, and -375 were upregulated in patients with prostate cancer compared with individuals without malignancy. Mao et al 97 reported that miRNA-141 in peripheral blood, measured with quantitative real-time PCR, was significantly higher in patients with prostate cancer compared with patients with benign prostatic hypertrophy.…”