Diagnostic potential and future directions of matrix metalloproteinases as biomarkers in gingival crevicular fluid of oral and systemic diseases

Zhang, Fan; Liu, Enyan; Radaic, Allan; Yu, Xiaotong; Yang, Shuting; Chen, Yu; Xiao, Shimeng; Ye, Changchang

doi:10.1016/j.ijbiomac.2021.07.165

Cited by 15 publications

(17 citation statements)

References 106 publications

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“…Unlike other collagenases, MMP-13 is a strong gelatinase with telopeptides activity. 37 , 38 However, there are several other, more specific markers that require further investigation. MMP-13 is a collagenase that has a relatively limited appearance in adult normal tissue.…”

Section: Discussionmentioning

confidence: 99%

Pre-Clinical Study: Immunohistochemical evaluation of matrix metalloproteinase-13 on rabbit (Oryctolagus cuniculus) socket healing after application of platelet-rich fibrin with and without hydroxyapatite

Damayanti

Rachmawati

2022

F1000Res

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Background: Tissue engineering technology has been used globally and proven to accelerate wound healing. This study aimed to analyse the effect of adding hydroxyapatite (HA) as a scaffold to platelet-rich fibrin (PRF) as a growth factor in accelerating the wound healing process as seen from the expression of matrix metalloproteinase-13 (MMP-13). Methods: This research is an animal experiment conducted on 18 rabbits (Oryctolagus cuniculus). Rabbits were randomly divided into the following three groups of treatment: (G1) the application of PRF group, (G2) the application of PRF+HA group and (C) the control group without any application. Furthermore, each treatment group was split randomly into three groups of observation time. Periodontal tissue biopsy was performed to analyse the histopathological features that were examined on the basis of the level of MMP-13 immunoexpression. Results: MMP-13 immunoexpression in the PRF+HA group showed better histoscore results, indicating a substantial reduction in MMP-13 values compared with other groups. The healing process was shown to increase with increasing observation time (p<0.05), and the PRF+HA group outperformed the PRF and control groups. On day 3, MMP-13 exhibited a dark brown colour of Immunohistochemistry (IHC), which indicated an increase in the expression value of MMP-13 in the early stages of healing, namely, inflammation. On day 14, light brown IHC was seen, especially in group 2, as a reference that the remodeling process had begun. Conclusions: This study indicates that the administration of PRF and HA was capable of reducing the MMP-13 expression that significantly accelerates the socket healing process. Hydroxyapatite is an alloplastic material that has inherent bioactive properties that support osteoconduction, can bind MMPs, and showed faster healing results based on the observation time as documented by immunohistochemistry.

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Section: Discussionmentioning

confidence: 99%

Pre-Clinical Study: Immunohistochemical evaluation of matrix metalloproteinase-13 on rabbit (Oryctolagus cuniculus) socket healing after application of platelet-rich fibrin with and without hydroxyapatite

Damayanti

Rachmawati

2022

F1000Res

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“…An important role in the onset of endocrine periodontopathy is played by not only local factors within the oral cavity, but also an abnormal immune response at the level of periodontium [ 109 , 110 ]. Bacteria in the subgingival biofilm cause the migration of PMNs from the gingival ditch into the tissues, with the consecutive release of chemotactic factors for the circulating neutrophils [ 111 , 112 , 113 , 114 , 115 , 116 , 117 ]. Macrophages play an important role due to their main function, namely phagocytosis of pathogenic germs and cellular debris, and subsequently present the antigen information to B and T lymphocytes, triggering the adaptive specific immune response; macrophages also release cytokines such as IL-1, IL-17, tumor necrosis factor α (TNF), leukotrienes, and prostaglandins with a role in tissue destruction, finally causing alveolar bone resorption [ 111 , 112 , 113 , 114 , 115 , 116 , 117 ].…”

Section: Methodsmentioning

confidence: 99%

“…Bacteria in the subgingival biofilm cause the migration of PMNs from the gingival ditch into the tissues, with the consecutive release of chemotactic factors for the circulating neutrophils [ 111 , 112 , 113 , 114 , 115 , 116 , 117 ]. Macrophages play an important role due to their main function, namely phagocytosis of pathogenic germs and cellular debris, and subsequently present the antigen information to B and T lymphocytes, triggering the adaptive specific immune response; macrophages also release cytokines such as IL-1, IL-17, tumor necrosis factor α (TNF), leukotrienes, and prostaglandins with a role in tissue destruction, finally causing alveolar bone resorption [ 111 , 112 , 113 , 114 , 115 , 116 , 117 ]. In the presence of a bacterial infection, PMNs release a high amount of metalloproteinase (MMP) in an attempt to neutralize the pathogens invading the periodontium [ 111 , 112 , 113 , 114 , 115 , 116 , 117 ].…”

Section: Methodsmentioning

confidence: 99%

“…Macrophages play an important role due to their main function, namely phagocytosis of pathogenic germs and cellular debris, and subsequently present the antigen information to B and T lymphocytes, triggering the adaptive specific immune response; macrophages also release cytokines such as IL-1, IL-17, tumor necrosis factor α (TNF), leukotrienes, and prostaglandins with a role in tissue destruction, finally causing alveolar bone resorption [ 111 , 112 , 113 , 114 , 115 , 116 , 117 ]. In the presence of a bacterial infection, PMNs release a high amount of metalloproteinase (MMP) in an attempt to neutralize the pathogens invading the periodontium [ 111 , 112 , 113 , 114 , 115 , 116 , 117 ]. Overproduction of MMPs leads to collagen (the main component of the periodontium) being damaged; thus, PMNs, which normally play a part in the organism’s defense, are the factor deteriorating the periodontium [ 111 , 112 , 113 , 114 , 115 , 116 , 117 ].…”

Section: Methodsmentioning

confidence: 99%

“…In the presence of a bacterial infection, PMNs release a high amount of metalloproteinase (MMP) in an attempt to neutralize the pathogens invading the periodontium [ 111 , 112 , 113 , 114 , 115 , 116 , 117 ]. Overproduction of MMPs leads to collagen (the main component of the periodontium) being damaged; thus, PMNs, which normally play a part in the organism’s defense, are the factor deteriorating the periodontium [ 111 , 112 , 113 , 114 , 115 , 116 , 117 ]. MMPs are endopeptidase enzymes, proteases with a role in the extracellular matrix, and are released by the epithelial keratinocytes, fibroblasts, macrophages, and leukocytes [ 111 , 112 , 113 , 114 , 115 , 116 , 117 ].…”

Section: Methodsmentioning

confidence: 99%

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New Approach to Addison Disease: Oral Manifestations Due to Endocrine Dysfunction and Comorbidity Burden

et al. 2022

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This review highlights oral anomalies with major clinical impact in Addison disease (AD), including dental health and dermatologic features, through a dual perspective: pigmentation issues and AD comorbidities with oral manifestations. Affecting 92% of AD patients, cutaneomucosal hyperpigmentation is synchronous with or precedes general manifestations by up to a decade, underlying melanocytic infiltration of the basal epidermal layer; melanophages in the superficial dermis; and, rarely, acanthosis, perivascular lymphocytic infiltrate, and hyperkeratosis. Intraoral pigmentation might be the only sign of AD; thus, early recognition is mandatory, and biopsy is helpful in selected cases. The buccal area is the most affected location; other sites are palatine arches, lips, gums, and tongue. Pigmented oral lesions are patchy or diffuse; mostly asymptomatic; and occasionally accompanied by pain, itchiness, and burn-like lesions. Pigmented lingual patches are isolated or multiple, located on dorsal and lateral areas; fungiform pigmented papillae are also reported in AD individuals. Dermoscopy examination is particularly indicated for fungal etiology; yet, it is not routinely performed. AD’s comorbidity burden includes the cluster of autoimmune polyglandular syndrome (APS) type 1 underlying AIRE gene malfunction. Chronic cutaneomucosal candidiasis (CMC), including oral CMC, represents the first sign of APS1 in 70–80% of cases, displaying autoantibodies against interleukin (IL)-17A, IL-17F ± IL-22, and probably a high mucosal concentration of interferon (IFN)-γ. CMC is prone to systemic candidiasis, representing a procarcinogenic status due to Th17 cell anomalies. In APS1, the first cause of mortality is infections (24%), followed by oral and esophageal cancers (15%). Autoimmune hypoparathyroidism (HyP) is the earliest endocrine element in APS1; a combination of CMC by the age of 5 years and dental enamel hypoplasia (the most frequent dental complication of pediatric HyP) by the age of 15 is an indication for HyP assessment. Children with HyP might experience short dental roots, enamel opacities, hypodontia, and eruption dysfunctions. Copresence of APS-related type 1 diabetes mellitus (DM) enhances the risk of CMC, as well as periodontal disease (PD). Anemia-related mucosal pallor is related to DM, hypothyroidism, hypogonadism, corresponding gastroenterological diseases (Crohn’s disease also presents oral ulceration (OU), mucogingivitis, and a 2-3 times higher risk of PD; Biermer anemia might cause hyperpigmentation by itself), and rheumatologic diseases (lupus induces OU, honeycomb plaques, keratotic plaques, angular cheilitis, buccal petechial lesions, and PD). In more than half of the patients, associated vitiligo involves depigmentation of oral mucosa at different levels (palatal, gingival, alveolar, buccal mucosa, and lips). Celiac disease may manifest xerostomia, dry lips, OU, sialadenitis, recurrent aphthous stomatitis and dental enamel defects in children, a higher prevalence of caries and dentin sensitivity, and gingival bleeding. Oral pigmented lesions might provide a useful index of suspicion for AD in apparently healthy individuals, and thus an adrenocorticotropic hormone (ACTH) stimulation is useful. The spectrum of autoimmune AD comorbidities massively complicates the overall picture of oral manifestations.

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Pulpal Diagnosis

Galicia,

Khan

2024

Vital Pulp Treatment

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Diagnostic potential and future directions of matrix metalloproteinases as biomarkers in gingival crevicular fluid of oral and systemic diseases

Cited by 15 publications

References 106 publications

Pre-Clinical Study: Immunohistochemical evaluation of matrix metalloproteinase-13 on rabbit (Oryctolagus cuniculus) socket healing after application of platelet-rich fibrin with and without hydroxyapatite

Pre-Clinical Study: Immunohistochemical evaluation of matrix metalloproteinase-13 on rabbit (Oryctolagus cuniculus) socket healing after application of platelet-rich fibrin with and without hydroxyapatite

New Approach to Addison Disease: Oral Manifestations Due to Endocrine Dysfunction and Comorbidity Burden

Pulpal Diagnosis

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