Diagnostic Potential of Autophagy-5 Protein, Apolipoprotein B-48, and Oxidative Stress Markers in Serum of Patients with Early-Stage Ischemic Stroke

Ajoolabady, Amir; Shademan, Behrouz; Avcı, Çığır Biray; Nikanfar, Masoud; Nourazarian, Alireza; Laghousi, Delara

doi:10.1016/j.wneu.2022.08.063

Cited by 8 publications

(9 citation statements)

References 20 publications

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“… 23 Currently, only one clinical study illustrates that serum ATG5 is elevated in patients with early‐stage ischemic stroke than controls. 18 In this study, serum ATG5 was elevated in AIS patients (vs. HCs), and it was positively linked with hyperlipidemia and NIHSS score in these patients. The potential explanations might be those (1) Elevated serum ATG5 contributed to more extensive neuronal death by accelerating neuronal autophagy, resulting in severer neurological dysfunction in AIS patients.…”

Section: Discussionmentioning

confidence: 47%

“…ATG5 plays an important role in the formation of autophagosomes 22 ; and the latter accumulate in cerebral tissue shortly after AIS 23 . Currently, only one clinical study illustrates that serum ATG5 is elevated in patients with early‐stage ischemic stroke than controls 18 . In this study, serum ATG5 was elevated in AIS patients (vs. HCs), and it was positively linked with hyperlipidemia and NIHSS score in these patients.…”

Section: Discussionmentioning

confidence: 50%

“…Another study indicates that increased ATG5 may lead to aggravated infarct lesion and neurological damage in AIS via facilitating ferroptosis 15 . Clinically, a study notices that serum ATG5 is higher in AIS patients compared to controls, which suggests the diagnostic potential of ATG5 in AIS 18 . However, the longitudinal change of ATG5 and its ability in predicting clinical outcomes of AIS patients is yet to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

“… 15 Clinically, a study notices that serum ATG5 is higher in AIS patients compared to controls, which suggests the diagnostic potential of ATG5 in AIS. 18 However, the longitudinal change of ATG5 and its ability in predicting clinical outcomes of AIS patients is yet to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Autophagy‐related 5 in acute ischemic stroke: Variation and linkage with neurofunction, and survival

Chen,

Wu,

Zhang

et al. 2024

Ann Clin Transl Neurol

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ObjectiveAutophagy‐related 5 (ATG5) facilitates the pathologic process of acute ischemic stroke (AIS) via multiple ways. This study aimed to identify the association of serum ATG5 with clinical outcomes in AIS patients.MethodsSerum ATG5 from 280 AIS patients were detected at admission, Day (D)1, D3, D7, D30, and D90 after admission by enzyme‐linked immunosorbent assay. The median (interquartile range) follow‐up was 21.1 (5.9–43.9) months. Another 50 healthy controls (HCs) were also enrolled for serum ATG5 determination.ResultsATG5 was elevated (p < 0.001) (vs. HCs), and positively correlated with hyperlipidemia (p = 0.016), and the national institutes of health stroke scale score (p = 0.001) in AIS patients. Interestingly, ATG5 was increased from admission to D1, but gradually decreased until D90 (p < 0.001). Besides, 85 (30.4%) and 195 (69.6%) AIS patients were assessed as modified Rankin Scale (mRS) >2 and mRS ≤2 at D90, respectively. ATG5 at admission, D1, D3, D30, and D90 was elevated in AIS patients with mRS >2 versus those with mRS ≤2 (all p < 0.050). ATG5 at admission, D1, D3, D7, D30, or D90 was elevated in relapsed (vs. non‐relapsed) or died (vs. survived) AIS patients (all p < 0.050). Recurrence‐free survival was shortened in AIS patients with high (≥52.0 ng/mL) ATG5 versus those with low (<52.0 ng/mL) ATG5 at admission, D3, D7, and D30 (all p < 0.050); overall survival was shorter in AIS patients with high (vs. low) ATG5 at D7 and D30 (both p < 0.050).InterpretationSerum ATG5 elevates at first, thereafter gradually declines, whose elevation associates with neurological dysfunction, recurrence, and death risk in AIS patients.

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Section: Discussionmentioning

confidence: 47%

Section: Discussionmentioning

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Autophagy‐related 5 in acute ischemic stroke: Variation and linkage with neurofunction, and survival

Chen,

Wu,

Zhang

et al. 2024

Ann Clin Transl Neurol

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“…Hsa-miR-181a-5p is associated with inflammation, upregulated in response to TGFβ signaling [52], and has been identified as a critical regulatory miRNA in ischemic strokes [53]. Serum levels of the protein encoded by its gene targets ATG5 is presented as a biomarker for AIS patients [54], while another gene target, ATM is involved in neuroprotection in the initiation of protective mechanisms in ischemic preconditioning but promotes neuronal cell death following the ischemic insult [55]. Additionally, upregulation of miR-222-3p was previously observed in acute myocardial infarction patients [56].…”

Section: Discussionmentioning

confidence: 99%

Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack Patients

Toor

Al-Dous

Parray

et al. 2022

IJMS

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Transient ischemic attack (TIA) refers to a momentary neurologic deficit caused by focal cerebral, spinal or retinal ischemic insult. TIA is associated with a high risk of impending acute ischemic stroke (AIS), a neurologic dysfunction characterized by focal cerebral, spinal or retinal infarction. Understanding the differences in molecular pathways in AIS and TIA has merit for deciphering the underlying cause for neuronal deficits with long-term effects and high risks of morbidity and mortality. In this study, we performed comprehensive investigations into the circulating microRNA (miRNA) profiles of AIS (n = 191) and TIA (n = 61) patients. We performed RNA-Seq on serum samples collected within 24 hrs of clinical diagnosis and randomly divided the study populations into discovery and validation cohorts. We identified a panel of 11 differentially regulated miRNAs at FDR < 0.05. Hsa-miR-548c-5p, -20a-5p, -18a-5p, -484, -652-3p, -486-3p, -24-3p, -181a-5p and -222-3p were upregulated, while hsa-miR-500a-3p and -206 were downregulated in AIS patients compared to TIA patients. We also probed the previously validated gene targets of our identified miRNA panel to highlight the molecular pathways affected in AIS. Moreover, we developed a multivariate classifier with potential utilization as a discriminative biomarker for AIS and TIA patients. The underlying molecular pathways in AIS compared to TIA may be explored further in functional studies for therapeutic targeting in clinical translation.

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Autophagy-related proteins: Potential diagnostic and prognostic biomarkers of aging-related diseases

Miceli

Leri

Stefani

et al. 2023

Ageing Research Reviews

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Diagnostic Potential of Autophagy-5 Protein, Apolipoprotein B-48, and Oxidative Stress Markers in Serum of Patients with Early-Stage Ischemic Stroke

Cited by 8 publications

References 20 publications

Autophagy‐related 5 in acute ischemic stroke: Variation and linkage with neurofunction, and survival

Autophagy‐related 5 in acute ischemic stroke: Variation and linkage with neurofunction, and survival

Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack Patients

Autophagy-related proteins: Potential diagnostic and prognostic biomarkers of aging-related diseases

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