Diagnostic Potential of Differentially Expressed Homer1 and Homer2 in Ischemic Stroke

Abstract

Background: Ischemic stroke (IS) is an extremely heterogeneous disease with variable pathogenesis. Due to the lack of early diagnostic marker, the mortality rate of IS remains high worldwide. The family of Homer plays an important role in the pathology of atherosclerotic plaque. In this study, we have investigated its expression pattern and clinical significance in IS. Methods: RT-qPCR was performed to detect the expression of Homer1, Homer2, and Homer3. Results: We found that the mRNA levels of Homer1 (p<0.001) and Homer2 (p<0.001), but not Homer3, in large-artery atherosclerosis (LAA) strokes were significantly upregulated than those in non-LAA strokes and controls. Multinomial logistic regression analyses showed that, although none of the Homer was associated with non-LAA strokes, higher Homer1 (adjusted OR=1.337, 95% CI: 1.227-1.458) and Homer2 (adjusted OR=1.099, 95% CI: 1.062-1.138) levels showed significant associations with increased odds of having LAA stroke, compared with the controls. The receiver operating characteristic (ROC) curves showed that the combination of Homer1 and Homer2 had a better diagnostic accuracy to differentiate LAA strokes from non-LAA strokes and controls, and the sensitivity and specificity ratios were 80.5%/90.4% and 98.0%/70.3%, respectively. Conclusion: Our data suggested that Homer1 and Homer2 might be considered as novel diagnostic biomarkers for LAA stroke.

Long Non-Coding RNA MEG3 Promotes Apoptosis of Vascular Cells and is Associated with Poor Prognosis in Ischemic Stroke

“…IS diagnosis is based primarily on imaging and neurological impairment. Unfortunately, current intervention and treatment of IS are still limited, including the following aspects: (1) Ischemic damage in brain tissue is not obvious within 24h after ischemic stroke by computed tomography (CT), and CT is mainly used to quickly eliminate intracranial tumors or cerebral hemorrhage 2 ) . (2) Magnetic resonance imaging (MRI) has higher sensitivity and specificity than CT, but most medical institutions in China cannot conduct MRI examinations 2 ) .…”

“…Unfortunately, current intervention and treatment of IS are still limited, including the following aspects: (1) Ischemic damage in brain tissue is not obvious within 24h after ischemic stroke by computed tomography (CT), and CT is mainly used to quickly eliminate intracranial tumors or cerebral hemorrhage 2 ) . (2) Magnetic resonance imaging (MRI) has higher sensitivity and specificity than CT, but most medical institutions in China cannot conduct MRI examinations 2 ) . (3) A recent systematic review suggested that the survival rate of IS patients who can receive thrombolytic therapy within 6h of symptom onset can be increased by 46.0%, and the disability rate caused by IS can be reduced by 42.1% 3 ) .…”

Long non-coding RNA MEG3 alters neuronal apoptosis and is associated with poor prognosis in ischemic stroke: based on basic and prospective studies

“…IS diagnosis is based primarily on imaging and neurological impairment. Unfortunately, current intervention and treatment of IS is still limited, including the following aspects: (1) Ischemic damage in brain tissue is not obvious within 24 hours after ischemic stroke by CT, and CT is mainly used to quickly eliminate intracranial tumors or cerebral hemorrhage [2]. (2) MRI has higher sensitivity and specificity than CT, but most medical institutions in China do not have the ability to conduct MRI examinations [2].…”

“…Unfortunately, current intervention and treatment of IS is still limited, including the following aspects: (1) Ischemic damage in brain tissue is not obvious within 24 hours after ischemic stroke by CT, and CT is mainly used to quickly eliminate intracranial tumors or cerebral hemorrhage [2]. (2) MRI has higher sensitivity and specificity than CT, but most medical institutions in China do not have the ability to conduct MRI examinations [2]. (3) A recent systematic review suggested that the survival rate of IS patients who can receive thrombolytic therapy within 6 hours of symptom onset can be increased by 46.0%, and the disability rate caused by IS can be reduced by 42.1% [3].…”