Diagnostic potential of<i>Fasciola gigantica</i>-derived 14.5 kDa fatty acid binding protein in the immunodiagnosis of bubaline fascioliasis

Allam, Gamal; Bauomy, Ibraheem Rabia; Hemyeda, Z.M.; Diab, T.; Sakran, Thabet

doi:10.1017/s0022149x12000168

Cited by 4 publications

(5 citation statements)

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“…Despite its absence from F. hepatica exosome-like vesicles, FABP isoform V shows potential as a diagnostic or vaccine candidate with strong IgM and IgG responses seen in pooled bovine infection sera. As diagnostics, Fasciola FABPs show promise with two studies by Allam and colleagues, suggesting FABP for the diagnosis of F. gigantica infections in both buffalo and man. , In both cases FABPs were purified from crude adult worm extracts, likely to contain multiple FABP isoforms. Thus, FABP isoforms I–III are likely to dominate the preparation, but the presence of FABP IV–VII cannot be discounted.…”

Section: Discussionmentioning

confidence: 99%

“…As diagnostics, Fasciola FABPs show promise with two studies by Allam and colleagues, suggesting FABP for the diagnosis of F. gigantica infections in both buffalo and man. 63,64 In both cases FABPs were purified from crude adult worm extracts, likely to contain multiple FABP isoforms. Thus, FABP isoforms I−III are likely to dominate the preparation, but the presence of FABP IV−VII cannot be discounted.…”

Section: ■ Discussionmentioning

confidence: 99%

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Exploring and Expanding the Fatty-Acid-Binding Protein Superfamily in Fasciola Species

et al. 2016

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The liver flukes Fasciola hepatica and F. gigantica infect livestock worldwide and threaten food security with climate change and problematic control measures spreading disease. Fascioliasis is also a foodborne disease with up to 17 million humans infected. In the absence of vaccines, treatment depends on triclabendazole (TCBZ), and overuse has led to widespread resistance, compromising future TCBZ control. Reductionist biology from many laboratories has predicted new therapeutic targets. To this end, the fatty-acid-binding protein (FABP) superfamily has proposed multifunctional roles, including functions intersecting vaccine and drug therapy, such as immune modulation and anthelmintic sequestration. Research is hindered by a lack of understanding of the full FABP superfamily complement. Although discovery studies predicted FABPs as promising vaccine candidates, it is unclear if uncharacterized FABPs are more relevant for vaccine formulations. We have coupled genome, transcriptome, and EST data mining with proteomics and phylogenetics to reveal a liver fluke FABP superfamily of seven clades: previously identified clades I-III and newly identified clades IV-VII. All new clade FABPs were analyzed using bioinformatics and cloned from both liver flukes. The extended FABP data set will provide new study tools to research the role of FABPs in parasite biology and as therapy targets.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Exploring and Expanding the Fatty-Acid-Binding Protein Superfamily in Fasciola Species

et al. 2016

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“…The enzyme substrate reaction was stopped by adding 50 μl/well of 8 n H 2 SO 4 . The absorbance was measured at 492 nm using an ELISA reader (Bio-Rad Microplate Reader) (Allam et al , 2012).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of purified 27.5 kDa protoscolex antigen-based ELISA for the detection of circulating antigens and antibodies in sheep and human hydatidosis

et al. 2014

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Hydatidosis is a zoonotic disease caused by the larval stage of Echinococcus granulosus, and the diagnosis of hydatidosis to date remains unresolved despite the development of many serological techniques. The present study aimed to develop an antigen-based enzyme-linked immunosorbent assay (ELISA) using IgG anti-27.5 kDa protoscolex antigen (27.5 PA) for measuring circulating protoscolex antigen (CPA), for comparison with an antibody detection assay, in sera of naturally infected sheep and humans in highly endemic areas in Egypt. In sheep, the sensitivity of ELISA in detecting anti-27.5 PA IgG and CPA was 75.0 and 60.0%, respectively, and the recorded specificity was 80.0 and 88.0%, respectively. In humans, the sensitivity of ELISA in detecting anti-27.5 PA IgG and CPA was 62.5 and 52.5%, respectively, while the specificity of the assay was 66.7 and 75.0%, respectively. In conclusion, an antibody detection assay is still superior and is more sensitive than an antigen detection assay, especially in diagnosing an active infection in which hydatid cysts are predominant. An antigen detection assay may be a useful approach to assessment of the efficacy of treatment, especially after removal of the cyst. Further studies are recommended to improve the diagnostic efficacy of an antigen-based ELISA method by using a highly purified recombinant antigen.

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“…Several F. hepatica purified antigens (17,18) and recombinant antigens have been employed to enhance the specificity of diagnostic assays (11,19,20). The most notable are cathepsin L, the major protease involved in F. hepatica virulence (21), fatty acid-binding proteins (FABPs) (22)(23)(24), and saposin-like proteins (FhSAP2) (25), which have been documented as useful immunodiagnostic antigens for serological detection of fascioliasis. Although many serological methods have been published, only a few have been commercialized.…”

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confidence: 99%

Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis

et al. 2014

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Coprological examination based on egg detection in stool samples is currently used as the gold standard for the diagnosis of human fascioliasis. However, this method is not effective during the acute phase of the disease and has poor sensitivity during the chronic phase. Serodiagnosis has become an excellent alternative to coprological examination in efforts to combat the effects of fascioliasis on human and animal health. Two novel recombinant Fasciola hepatica proteins, i.e., a ferritin (FhFtn-1) and a tegument-associated protein (FhTP16.5), were used as antigens to develop in-house enzyme-linked immunosorbent assay (ELISA) methods. The assays were optimized and validated using 152 serum samples from humans with a known infection status, including healthy subjects, patients with chronic fascioliasis, and patients with other parasitic diseases. The FhFtn-1 ELISA was shown to be 96.6% sensitive and 95.7% specific; the respective parameters for the FhTP16.5 ELISA were 91.4% and 92.4%. The performances of the FhFtn-1 and FhTP16.5 ELISAs were compared with that of an available commercial test (the DRG test) using a subset of serum samples. Our in-house tests were slightly more sensitive than the DRG test in detecting antibodies against F. hepatica, but the differences were not statistically significant. In conclusion, the present study provides evidence for the potential of the FhFtn-1 and FhTP16.5 ELISAs as diagnostic tools for human fascioliasis, as might be implemented in conjunction with standard assays for large-scale screenings in areas where the disease is endemic and for the detection of occasional cases in clinical laboratories.

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Diagnostic potential ofFasciola gigantica-derived 14.5 kDa fatty acid binding protein in the immunodiagnosis of bubaline fascioliasis

Cited by 4 publications

References 35 publications

Exploring and Expanding the Fatty-Acid-Binding Protein Superfamily in Fasciola Species

Exploring and Expanding the Fatty-Acid-Binding Protein Superfamily in Fasciola Species

Evaluation of purified 27.5 kDa protoscolex antigen-based ELISA for the detection of circulating antigens and antibodies in sheep and human hydatidosis

Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis

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