Diagnostic Potential of IgG and IgA Responses to Mycobacterium tuberculosis Antigens for Discrimination among Active Tuberculosis, Latent Tuberculosis Infection, and Non-Infected Individuals

Lee, Ji Yeon; Kim, Byoung Jun; Koo, Hyeon Kyoung; Kim, Jung-Hyun; Kim, Jee Min; Kook, Yoon Hoh; Kim, Bumjoon J.

doi:10.3390/microorganisms8070979

Cited by 17 publications

(11 citation statements)

References 37 publications

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“…In contrast, anti-Ag85B IgG levels changed according to the disease status. Majority of the studies conducted on Ag85B were done in humans and mice 31 , 39 – 42 , but very few on elephants. Ag85B is a mycolyltransferase secreted by mycobacteria and is involved in the final stage of mycobacterial cell wall assembly by transferring mycolic acid to sugars like trehalose 39 .…”

Section: Discussionmentioning

confidence: 99%

Monitoring IgG against Mycobacterium tuberculosis proteins in an Asian elephant cured of tuberculosis that developed from long-term latency

Ishikawa

Ozeki

Suga

et al. 2022

Sci Rep

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Tuberculosis (TB) is fatal in elephants, hence protecting elephants from TB is key not only in the conservation of this endangered animal, but also to prevent TB transmission from elephants to humans. Most human TB cases arise from long-term asymptomatic infections. Significant diagnostic challenges remain in the detection of both infection and disease development from latency in elephants due to their huge bodies. In this study, we assessed cryopreserved sera collected for over 16 years, from the first Japanese treatment case of elephant TB. Semi-quantification of IgG levels to 11 proteins showed high detection levels of 3 proteins, namely ESAT6/CFP10, MPB83 and Ag85B. The level of IgG specific to these 3 antigens was measured longitudinally, revealing high and stable ESAT6/CFP10 IgG levels regardless of onset or treatment. Ag85B-specifc IgG levels were largely responsive to onset or treatment, while those of MPB83 showed intermediate responses. These results suggest that ESAT6/CFP10 is immunodominant in both asymptomatic and symptomatic phases, making it useful in the detection of infection. On the other hand, Ag85B has the potential to be a marker for the prediction of disease onset and in the evaluation of treatment effectiveness in elephants.

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Section: Discussionmentioning

confidence: 99%

Monitoring IgG against Mycobacterium tuberculosis proteins in an Asian elephant cured of tuberculosis that developed from long-term latency

Ishikawa

Ozeki

Suga

et al. 2022

Sci Rep

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“…Tree is a nonparametric statistical procedure containing classification by using a set of if–then-else logical conditions to assign unknown features to a predefined category. Algorithms for constructing tree work are from top to down, by choosing a variable at each step that best splits the set of items 25 . Tree creates partition recursively to increase purity in the direction to lower the impurity using Gini index.…”

Section: Methodsmentioning

confidence: 99%

Differential features of chronic cough according to etiology and the simple decision tree for predicting causes

Koo

Bae

Moon

et al. 2021

Sci Rep

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Finding etiology of chronic cough is an essential part of treatment. Although guidelines include many laboratory tests for diagnosis, these are not possible in many primary care centers. We aimed to identify the characteristics and the differences associated with its cause to develop a clinical prediction model. Adult subjects with chronic cough who completed both Korean version of the Leicester Cough Questionnaire (K-LCQ) and COugh Assessment Test (COAT) were enrolled. Clinical characteristics of each etiology were compared using features included in questionnaires. Decision tree models were built to classify the causes. A total of 246 subjects were included for analysis. Subjects with asthma including cough variant asthma (CVA) suffered from more severe cough in physical and psychological domains. Subjects with eosinophilic bronchitis (EB) presented less severe cough in physical domain. Those with gastro-esophageal reflux disease (GERD) displayed less severe cough in all 3 domains. In logistic regression, voice hoarseness was an independent feature of upper airway cough syndrome (UACS), whereas female sex, tiredness, and hypersensitivity to irritants were predictors of asthma/CVA; less hoarseness was a significant feature of EB, and feeling fed-up and hoarseness were less common characteristics of GERD. The decision tree was built to classify the causes and the accuracy was relatively high for both K-LCQ and COAT, except for UACS. Voice hoarseness, degree of tiredness, hypersensitivity to irritants and feeling fed-up are important features in determining the etiologies. The decision tree may further assists classifying the causes of chronic cough.

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“…The participants were enrolled, as described previously [ 22 ]. Briefly, 180 individuals, including 65 ATB patients, 53 LTBI patients, and 62 NI individuals, were enrolled at the National Medical Center, Seoul, Republic of Korea.…”

Section: Methodsmentioning

confidence: 99%

“…Antibody isotypes, IgA and IgG, serum levels were assessed while using an ELISA, as previously described with minor modifications [ 22 ]. Briefly, Corning 96-well Enzyme Immunoassay/Radio Immunoassay (EIA/RIA) plates (Corning Inc., Kennebunk, ME, USA) were coated overnight at 4 °C with a solution containing 5 μg/mL of MIF diluted in a 0.05 M carbonate-bicarbonate coating buffer.…”

Section: Methodsmentioning

confidence: 99%

Usefulness of the IgA and IgG Responses to Macrophage Migration Inhibitory Factor for the Diagnosis of Tuberculosis

Lee

Kim

et al. 2020

Diagnostics

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Serological tests offer the potential in order to improve the diagnosis of tuberculosis (TB). Macrophage migration inhibitory factor (MIF) plays a protective role in infection control in TB; however, to date, no studies on antibody responses to MIF have been reported. We measured immunoglobulin (Ig)A and IgG responses to MIF in individuals with either active tuberculosis (ATB; n = 65), latent tuberculosis (LTBI; n = 53), or in non-infected individuals (NI; n = 62). The QuantiFERON-TB Gold In-Tube (QFT-GIT) assay was used in order to screen for LTBI. The level of IgA against MIF was significantly lower in LTBI and ATB patients than in NI individuals, was significantly related to LTBI and ATB diagnosis, and it could discriminate between LTBI and ATB. In contrast, the level of IgG against MIF was significantly lower in LTBI patients than in NI individuals and was significantly related to LTBI diagnosis. Anti-MIF IgG levels were significantly lower in AFB-negative TB, minimal TB, and new ATB patients, than in the NI group. IgA and IgG levels against MIF both showed significant negative correlations with IFN-γ levels, as assessed using the QFT-GIT test. Although none of the antibodies could achieve high diagnostic predictive power individually, our results suggest the possibility of using IgA antibody responses to MIF in the diagnosis of LTBI and ATB.

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Diagnostic Potential of IgG and IgA Responses to Mycobacterium tuberculosis Antigens for Discrimination among Active Tuberculosis, Latent Tuberculosis Infection, and Non-Infected Individuals

Cited by 17 publications

References 37 publications

Monitoring IgG against Mycobacterium tuberculosis proteins in an Asian elephant cured of tuberculosis that developed from long-term latency

Monitoring IgG against Mycobacterium tuberculosis proteins in an Asian elephant cured of tuberculosis that developed from long-term latency

Differential features of chronic cough according to etiology and the simple decision tree for predicting causes

Usefulness of the IgA and IgG Responses to Macrophage Migration Inhibitory Factor for the Diagnosis of Tuberculosis

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