Diagnostic potential of LncRNAs-ANRIL and MIAT in the blood of patients with cerebral venous thrombosis

Abstract

Background: Long non-coding RNAs (LncRNAs) have been implicated as possible circulating stroke indicators. This study focused on the expression status of ANRILand MIAT in cerebral venous thrombosis (CVT) patients. Methods: In this study, fifty CVT patients and one hundred age/gender-matched individuals as controls were included. The circulating levels of ANRIL and MIATin the first 24 hours after admission were evaluated using the quantitative real-time polymerase chain reaction (RT-PCR) method. We compared the expression levels of ANRIL and MIAT between patients and controls using the independent two-sample t-test. Subgroup analysis was used toinvestigate the association of lncRNAs with clinical characteristics in CVT patients. ROC curve analyses conducted the diagnostic value of two lncRNAs in patient assessment. Results: The relative expression of lncRNAs ANRILand MIAT significantly decreased in patients compared to the control. ANRILand MIAT showed potential markers for discriminating CVT patients from the healthy controls with an AUC of 0.98 and 0.99, respectively. Conclusion: For the first time we found downregulation and diagnostic potential of LncRNAs-ANRILand MIAT in the blood of patients with CVT.