Diagnostic potential of Mab‐based ELISAs for antibodies to non‐structural proteins of foot‐and‐mouth disease virus to differentiate infection from vaccination

Brocchi, Emiliana; Diego, M. De; Berlinzani, A.; Gamba, D.; Simone, Franco

doi:10.1080/01652176.1998.9694957

Cited by 18 publications

(14 citation statements)

References 11 publications

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“…By contrast, the FMDV non-structural proteins 3A and 3C could not be detected in any of the tissues from animals after 28 days post contact infection (Table 2, Figure 3) [10], [11]. Antibodies specific for 3A and 3C readily detected infected cells in FMDV vesicles and lymph tissue during the acute phase of infection and in infected BHK-21 cells, co-localising with FMDV capsid (Figure S11 to S13).…”

Section: Resultsmentioning

confidence: 97%

Foot-and-Mouth Disease Virus Persists in the Light Zone of Germinal Centres

et al. 2008

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Foot-and-mouth disease virus (FMDV) is one of the most contagious viruses of animals and is recognised as the most important constraint to international trade in animals and animal products. Two fundamental problems remain to be understood before more effective control measures can be put in place. These problems are the FMDV “carrier state” and the short duration of immunity after vaccination which contrasts with prolonged immunity after natural infection. Here we show by laser capture microdissection in combination with quantitative real-time reverse transcription polymerase chain reaction, immunohistochemical analysis and corroborate by in situ hybridization that FMDV locates rapidly to, and is maintained in, the light zone of germinal centres following primary infection of naïve cattle. We propose that maintenance of non-replicating FMDV in these sites represents a source of persisting infectious virus and also contributes to the generation of long-lasting antibody responses against neutralising epitopes of the virus.

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Section: Resultsmentioning

confidence: 97%

Foot-and-Mouth Disease Virus Persists in the Light Zone of Germinal Centres

et al. 2008

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“…Since the original development, other tests became available to detect antibodies to NCPs [60][61][62][63]. At present, a number of commercial kits are available.…”

Section: Companion Serologic Tests For Evaluation Of Viral Activity Imentioning

confidence: 99%

Evolving perception on the benefits of vaccination as a foot and mouth disease control policy: contributions of South America

Bergmann

Malirat

Falczuk

2005

Expert Review of Vaccines

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Within the past decade, changes in perceptions on the benefits of vaccination as an appropriate tool to achieve complete foot and mouth disease eradication have become evident. The former negative view was derived from misconceptions, resulting mainly from the belief that vaccines are not entirely effective and that vaccination masks asymptomatic viral circulation. The advent in the 1990s of vaccination policies implemented within a strategic eradication plan in South America, and during recurrence of the disease in disease-free regions contributed towards generating more reliable and visible outcomes of vaccination programs, paving the way towards a new perception. Particularly relevant was the development and application of novel serodiagnostic approaches to assess silent viral circulation, irrespective of vaccination. The use in South America of vaccination allied to serosurveys to accompany viral clarification during eradication campaigns and after emergencies clearly established the importance of this control tool to stop the spread of viral infection. This alliance gave input to break many myths associated with the use of vaccines, including the belief that immunized carrier animals pose an epidemiologic risk. This experience launched new concepts that supported the internationally recognized status of foot and mouth disease-free regions with vaccination and the 'vaccination to live' policy as an alternative to 'stamping out'.

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“…A PI of Ͻ50% was considered negative, and a PI of Ն50% was considered positive for recent exposure (within the previous 6 to 12 months) (7,14). More specifically, a PI value of Ն50% but Ͻ70% was considered a weak positive result, and a PI value of Ն70% was considered a strong positive result (36).…”

Section: Methodsmentioning

confidence: 99%

Serological Survey for Foot-and-Mouth Disease Virus in Wildlife in Eastern Africa and Estimation of Test Parameters of a Nonstructural Protein Enzyme-Linked Immunosorbent Assay for Buffalo

Bronsvoort

Parida

Handel

et al. 2008

Clin Vaccine Immunol

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In this study we estimate the seroprevalence of foot-and-mouth disease virus (FMDV) in wildlife from eastern and central Africa. Sera were sourced from between 1994 and 2002 from a rinderpest surveillance program. Our study compared a nonstructural protein enzyme-linked immunosorbent assay (Cedi test) with a virus neutralization test. The study shows that there is only a low seroprevalence of FMDV in sampled nonbuffalo species. The seroprevalence in the Cape buffalo was high for SAT2, lower for SAT1, and lowest for SAT3. As the SAT2 serotype was most prevalent, the Cedi test largely reflected the occurrence of SAT2-positive animals. The results also suggest that SAT2 became dominant around 1998, with a large increase in seroprevalence. The sensitivity and specificity of the Cedi test were estimated by comparison to the combined virus neutralization test results from all three SAT tests. A Bayesian implementation of the Hui-Walter latent class model was used to estimate the test parameters. The model permits estimation in the absence of a gold standard test. The final model, using noninformative priors and assuming conditional independence of test performance, estimated Cedi test sensitivity at 87.7% and specificity at 87.3%. These estimates are similar to those for domestic bovines; they suggest that the Cedi test is a useful tool for screening buffalo for infection with the various serotypes of FMDV.

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Diagnostic potential of Mab‐based ELISAs for antibodies to non‐structural proteins of foot‐and‐mouth disease virus to differentiate infection from vaccination

Cited by 18 publications

References 11 publications

Foot-and-Mouth Disease Virus Persists in the Light Zone of Germinal Centres

Foot-and-Mouth Disease Virus Persists in the Light Zone of Germinal Centres

Evolving perception on the benefits of vaccination as a foot and mouth disease control policy: contributions of South America

Serological Survey for Foot-and-Mouth Disease Virus in Wildlife in Eastern Africa and Estimation of Test Parameters of a Nonstructural Protein Enzyme-Linked Immunosorbent Assay for Buffalo

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