Diagnostic predictability of miR-4535 and miR-1915–5p expression in amniotic fluid for foetal morbidity of infection

Yoshikawa, Kenichi; Kiyoshima, Chihiro; Hirakawa, Tsubasa; Urushiyama, Daichi; Fukagawa, Satoshi; Izuchi, Daisuke; Sanui, Ayako; Kurakazu, Masamitsu; Miyata, Koichiro; Nomiyama, Makoto; Setoue, Takashi; Nagamitsu, Shinichiro; Nabeshima, Kazuki; Hata, Kenichiro; Yasunaga, Shin’ichiro; Miyamoto, Shingo

doi:10.1016/j.placenta.2021.08.059

Cited by 6 publications

(1 citation statement)

References 41 publications

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“…Biomarkers from amniotic fluid and maternal plasma are considered powerful diagnostic tools for assessing fetal health and disease. Studies have shown that amniotic fluid ncRNA profiles play a significant role in clinical prediction in fetal infection and twin-twin transfusion syndrome recipients (Willner et al, 2021;Yoshikawa et al, 2021). In our previous publication, we found that some amniotic fluid lncRNAs (LINC00598, LINC01551, PWRN1, and GATA3-AS1) were differently expressed in VSD fetuses, which may be potential biomarkers for VSD fetuses (Wang et al, 2023).…”

Section: Introductionmentioning

confidence: 59%

Maternal body fluid lncRNAs serve as biomarkers to diagnose ventricular septal defect: from amniotic fluid to plasma

Wang,

Lin,

Wang

et al. 2023

Front. Genet.

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Background: Maternal body fluids contain abundant cell-free fetal RNAs which have the potential to serve as indicators of fetal development and pathophysiological conditions. In this context, this study aimed to explore the potential diagnostic value of maternal circulating long non-coding RNAs (lncRNAs) in ventricular septal defect (VSD).Methods: The potential of lncRNAs as non-invasive prenatal biomarkers for VSD was evaluated using quantitative polymerase chain reaction (qPCR) and receiver operating characteristic (ROC) curve analysis. The biological processes and regulatory network of these lncRNAs were elucidated through bioinformatics analysis.Results: Three lncRNAs (LINC00598, LINC01551, and GATA3-AS1) were found to be consistent in both maternal plasma and amniotic fluid. These lncRNAs exhibited strong diagnostic performance for VSD, with AUC values of 0.852, 0.957, and 0.864, respectively. The bioinformatics analysis revealed the involvement of these lncRNAs in heart morphogenesis, actin cytoskeleton organization, cell cycle regulation, and protein binding through a competitive endogenous RNA (ceRNA) network at the post-transcriptional level.Conclusion: The cell-free lncRNAs present in the amniotic fluid have the potential to be released into the maternal circulation, making them promising candidates for investigating epigenetic regulation in VSD.

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Section: Introductionmentioning

confidence: 59%