Diagnostic Procedures

Recent Advances in Pestivirus Research

“…Although most workers agree that each pestivirus is antigenically homogeneous, i.e. that serotypes of HCV, BVDV or BDV do not exist (Carbrey, 1988), analyses using polyclonal antisera have shown strain variations detectable by cross-neutralization tests within each pestivirus (Aynaud et al, 1974;Neukirch et al, 1980). Neutralization assays are also able to distinguish between HCV and BVDV, but not between BVDV and BDV.…”

“…Current routine procedures are tedious, expensive and often inaccurate. Discrimination between pestivirus strains by virological and serological means is difficult due to extensive serological crossreactions (Carbrey, 1988;Liess, 1988). Simple procedures for the detection of antibodies to pestiviruses in field sera have been lacking.…”

Extraction and epitopic analysis of the 53 kd envelope glycoprotein of bovine viral diarrhea virus

“…Neutralization assays distinguish BVDV from HCV (Sheffy, 1962), but do not distinguish between BVDV and BDV (Vantsis et al, 1976;Laude and Guelfi, 1979). The serologic cross relatedness of BVDV, and lack of distinct serotypes, is an accepted convention (Carbrey, 1988;Moennig et al, 1988;Cay et al, 1989). However, antigenic differences demonstrated by cross neutralization tests, using polyclonal sera (Aynaud et al, 1974;Neukirch et al, 1980) and monoclonal antibodies Magaret al, 1988;Corapi and Dubovi, 1990), indicate variation among isolates.…”