Diagnostic Reagents in Drug Allergy: Immunochemical Specificity in the 1,2-Diphenyl-Pyrazolidinedione Series

Kasper, M.; Schneider, Conrad H.; Rolli, Hanspeter; Angst, Brigitt D.; Weck, A.L. de

doi:10.1016/s0171-2985(86)80093-6

Cited by 9 publications

(8 citation statements)

References 9 publications

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“…The data presented here are an extension of n I ADOH I ADOH I II I I I I I 1 work published previously (4,5) and form a basis for evaluations of clinical diagnostic data.…”

mentioning

confidence: 72%

“…Anti-hapten-HSA antisera from out-bred guinea pigs and rabbits has been described previously Passive cutaneous anaphylaxis (PCA). The technique of Watanabe & Ovary (9) was used as described (4,5) for eliciting reactions in antihapten-HSA sensitized guinea pigs. ELISA technique has been described before using Buto-NHj+Cl" and Butaz-NHj+Cl" for coating the microtiter plates (4).…”

Section: Preparation Of N^ (6-aminohexanoyl)-4-amidoantipyrine (Phenamentioning

confidence: 99%

“…The technique of Watanabe & Ovary (9) was used as described (4,5) for eliciting reactions in antihapten-HSA sensitized guinea pigs. ELISA technique has been described before using Buto-NHj+Cl" and Butaz-NHj+Cl" for coating the microtiter plates (4). For inhibition studies the anti-Phenaz-HSA antiserum was used at a 1:6000 dilution.…”

Section: Preparation Of N^ (6-aminohexanoyl)-4-amidoantipyrine (Phenamentioning

confidence: 99%

“…Microtiter plates coated with Phenaz-NH3+Cl , Buto-NHj+Cl-or Butaz-NHj+Cl-by means of glutardialdehyde under defined conditions were reacted with anti-hapten antisera from the rabbit in graded dilutions in order to detect specific binding as described before (4). Fig.…”

Section: Elisa Testsmentioning

confidence: 99%

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Diagnosis of antibody‐mediated drug allergy

Schneider

Kasper

Weck

et al. 1987

Allergy

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Based on the 1-phenyl-2,3-dimethyl-3-pyrazolin-5-one series and on the 1,2-diphenyl-pyrazolidine-3,5-dione series of drugs, haptenic reagents and conjugates were synthesized and evaluated by passive cutaneous anaphylaxis in guinea pigs, and by ELISA tests using rabbit antisera against the haptens. No cross-reactivity between pyrazolinone and pyrazolidinedione haptenic reagents was found in any of the test systems. But also within each series, the animal antibodies showed rather strict specificities upon interaction with related haptens or drugs. These results are somewhat unexpected, because the haptens were used in connection with long and flexible spacer arms. Furthermore, they are not typical for certain other drug allergies. The strict specificity reduces the diagnostic potential of the haptenic reagents when used in serological tests or in skin testing.

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“…The data presented here are an extension of n I ADOH I ADOH I II I I I I I 1 work published previously (4,5) and form a basis for evaluations of clinical diagnostic data.…”

mentioning

confidence: 72%

Section: Preparation Of N^ (6-aminohexanoyl)-4-amidoantipyrine (Phenamentioning

confidence: 99%

Section: Preparation Of N^ (6-aminohexanoyl)-4-amidoantipyrine (Phenamentioning

confidence: 99%

Section: Elisa Testsmentioning

confidence: 99%

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Diagnosis of antibody‐mediated drug allergy

Schneider

Kasper

Weck

et al. 1987

Allergy

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“…Polystyrene microtitre plates (Dynatech) were coated with N 1 -Dncp±diaminohexane and used as described before [13,14]. The detecting antibodies goat anti-guinea pig IgG (H L) alkaline phosphatase and goat anti-mouse IgG (H L) alkaline phosphatase were obtained from Jackson Immunoresearch Laboratories, West Grove, PA. Titres were expressed as the highest reciprocal dilution (D r ) of the antisera giving an absorbance of 1.0 after 30 min incubation with 4-nitrophenylphosphate (1 mg/ml in 0.05 M carbonate buffer ± 1 mM MgCl 2 , pH 9.8).…”

Section: Elisamentioning

confidence: 99%

Molecular parameters in melittin immunogenicity

et al. 1997

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Based on immunogenicity studies, two T-cell epitopes in melittin were found to be functional in guinea pigs, one being centrally located, the other one residing in the C-terminal chain. In Balb/c mice only the central epitope was found to be active. A human T-cell clone was found by T-cell proliferation studies to employ strictly the C-terminal chain. Truncation of melittin peptides at the N-terminus did not markedly affect the capacity of guinea pigs to develop anti-IgG responses towards peptidic epitopes and towards a C-terminally attached haptenic group. Attachment of various substituents inside and outside the T-cell epitopic areas had no marked effect on antibody responses. In contrast, the substituents positioned within a T-cell epitope abolished T-cell proliferation. This difference between whole animal data and cellular in vitro responses is presently not understood.

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Immunogenicity of dinitrocarboxyphenylated melittin: The influence of C‐terminal chain shortening, N‐terminal substitution and prolin insertion at positions 5 and 10

Zhao

Rolli

Schneider

1995

Journal of Peptide Science

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Peptides derived from the bee-venom melittin were fitted with the haptenic group dinitrocarboxyphenyl (Dncp) and tested in out-bred guinea pigs for immunogenicity by measuring the IgG anti-Dncp antibody response by ELISA. Dncp-conjugates comprising virtually the entire melittin proved to be strong immunogens producing antibody responses comparable to those of proteins. Weak responses were obtained with considerably shortened sequences. Conjugates with N-terminal Dncp gave markedly reduced antibody responses compared to peptides with C-terminal Dncp. An N-terminal biotinyl substituent abolished the immune response whereas N-terminal lauryl and caprylyl had little effect. Insertion of L-proline into a hexadecapeptide conjugate abolishing the possibility of helix formation gave an immunogen to which individual animals clearly responded on a low level. Oligomerisation, but not the cytolytic activity of melittin peptides, may contribute to the immunogenicities observed.

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Diagnostic Reagents in Drug Allergy: Immunochemical Specificity in the 1,2-Diphenyl-Pyrazolidinedione Series

Cited by 9 publications

References 9 publications

Diagnosis of antibody‐mediated drug allergy

Diagnosis of antibody‐mediated drug allergy

Molecular parameters in melittin immunogenicity

Immunogenicity of dinitrocarboxyphenylated melittin: The influence of C‐terminal chain shortening, N‐terminal substitution and prolin insertion at positions 5 and 10

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