Diagnostic significance of colloid body deposition in direct immunofluorescence

Chularojanamontri, Leena; Tuchinda, Papapit; Triwongwaranat, Daranporn; Pinkaew, Sumruay; Kulthanan, Kanokvalai

doi:10.4103/0378-6323.66583

Cited by 15 publications

(23 citation statements)

References 21 publications

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“…Even in such circumstances, CK-903 can still assist in diagnosis by highlighting the pattern, quantity, and location of colloid body deposition which is usually different than that seen in LPP. 22 Our stain also has the significant additional advantages over DIF of low cost, short preparation time, and not needing a specialized fluorescent microscope to view staining.…”

Section: Discussionmentioning

confidence: 98%

“…22,26,28 Colloid bodies can be detected on direct immunofluorescence (DIF) staining in LPP as clusters of IgM or less commonly, IgA and C3. 7,22,28,29 Generally, colloid bodies are found in a perifollicular distribution, but they may also seem to reside within the interfollicular dermis in cases with epidermal involvement and occasionally in cases without epidermal involvement. A possible explanation for this latter finding is that the hair follicle actually lies in adjacent cuts and may be missed if only a limited number of sections are examined.…”

Section: Discussionmentioning

confidence: 99%

“…21 CKs, and specifically CK-10, are a major component of the colloid bodies that are strongly suggestive of LPP. [22][23][24] Staining for these CKs should therefore enable the visualization of colloid bodies even in the presence of significant inflammation and fibrosis. To assess the utility of this stain, we have performed a retrospective analysis of 18 cases of previously diagnosed LPP and 20 cases of similar appearing entities and assessed for positive staining of colloid bodies through CK-903.…”

Section: Introductionmentioning

confidence: 99%

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The Use of Anti-Keratin 903 Antibodies to Visualize Colloid Bodies and Diagnose Lichen Planopilaris

Lanoue

Yanofsky

Mercer

et al. 2016

The American Journal of Dermatopathology

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Cytokeratins are a major component of colloid bodies that are essentially diagnostic of lichen planopilaris (LPP). Here, the authors assess the ability of the cytokeratin 903 antibody (CK-903) to stain colloid bodies and differentiate LPP from other histologically similar appearing primary cicatricial alopecias. A retrospective review of all specimens submitted to the dermatopathology department over a 2-year window identified 18 cases of LPP and 20 cases of histologically similar appearing entities (discoid lupus erythematosus or central centrifugal cicatricial alopecia) through a combination of H&E, elastic van gieson, and periodic acid-schiff stains. All 38 samples were then prospectively stained with CK-903. Colloid bodies were identifiable in 3 of the 18 LPP cases based on H&E alone but were seen in 9 of 18 cases when CK-903 was used. There were no cases where colloid bodies were seen on H&E but not subsequently identified with CK-903. Additionally, there was no CK-903 staining in any of the 20 cases of similar appearing entities except 1 case of discoid lupus erythematosus, which is known to occasionally show colloid bodies. The authors conclude that CK-903 is a useful adjunctive tool that will allow for a quicker, less costly, and more accurate diagnosis of LPP given its ability identify colloid bodies even in the setting of significant inflammation and fibrosis and its advantages over direct immunofluorescence of low cost, short preparation time, and lack of need for a specialized fluorescent microscope.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Use of Anti-Keratin 903 Antibodies to Visualize Colloid Bodies and Diagnose Lichen Planopilaris

Lanoue

Yanofsky

Mercer

et al. 2016

The American Journal of Dermatopathology

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“…[7]. By light microscopy the apoptotic keratinocytes are evidenced as eosinophilic ovoid bodies found in the epidermis and subepidermal papillary regions in LP patients [36]. Colloid bodies (CB), also known as Civatte bodies, hyaline or cytoid bodies, reflecting apoptosis of keratinocytes and attenuating changes of intermediate filaments, were described several decades ago by researchers conducting electron microscopy investigations and TUNEL reaction analysis [37,38,39,40,41,42].…”

Section: Introductionmentioning

confidence: 99%

Eradication of damaged keratinocytes in cutaneous lichen planus forms demonstrated by evaluation of epidermal and follicular expression of CK15, indices of apoptosis and regulatory protein S100

Upeniece

Groma

Skuja

et al. 2016

pjp

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The study of cytoskeleton arrangement and its contribution to survival of cell-tocell contacts appears to be essential for understanding of numerous cellular and tissue processes. Applying CK15, S100 labeling and TUNEL reaction to cutaneous lichen planus subtypes, we found CK15 expression in the outer and inner root sheath of hair follicles, the basal epidermal layer, and eccrine glands. Its follicular expression was decreased in nearby inflammatory infiltrates. The CK15 immunopositivity was mostly described as weak (92.3%) for lichen planus but equally subdivided into weak, moderate and strong in lichen planopilaris (χ 2 = 32.514; df = 4; p < 0.001). The greatly varying apoptotic index was the highest in the lichen planopilaris involving the scalp: 81.2 ±10.7; 87.8 ±10.7 and 88.0 ±10.5 for the basal, spinous and upper epidermal layers, respectively. S100 positive epidermal and follicular cells did not differ in the lesions demonstrated in the study groups; still immunoreactivity was more pronounced in the scalp region of lichen planopilaris. Damage of cell-to-cell contacts was confirmed by electron microscopy. Apart from immunocyte-mediated keratinocyte death, cytoskeleton-based injury and loss of cellto-cell and matrix contacts may be of great importance, leading to eradication of degrading cells and thus contributing to the pathogenesis of lichen planus.

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“…Civatte Bodies [2] These might be referred as colloid Bodies. However, some references refer to colloid Bodies as apoptotic cell remnants in papillary dermis, whereas Civatte bodies as apoptotic cell remnants in epidermis.…”

mentioning

confidence: 99%

Eponyms in the dermatology literature linked to ''Bodies'', seen in Skin biopsies

Aboud¹,

Aboud²

2013

Our Dermatol Online

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Diagnostic significance of colloid body deposition in direct immunofluorescence

Cited by 15 publications

References 21 publications

The Use of Anti-Keratin 903 Antibodies to Visualize Colloid Bodies and Diagnose Lichen Planopilaris

The Use of Anti-Keratin 903 Antibodies to Visualize Colloid Bodies and Diagnose Lichen Planopilaris

Eradication of damaged keratinocytes in cutaneous lichen planus forms demonstrated by evaluation of epidermal and follicular expression of CK15, indices of apoptosis and regulatory protein S100

Eponyms in the dermatology literature linked to ''Bodies'', seen in Skin biopsies

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