Diagnostic Techniques and Sample Handling

Berzins, Ilze K.; Smolowitz, Roxanna; Lewbart, Gregory A.

doi:10.1002/9781119569831.ch25

Cited by 4 publications

(2 citation statements)

References 94 publications

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“…Afterward, the samples were fixed in 10% neutral buffered formalin. Similarly, Berzins et al (2021) suggested that the invertebrate samples should be kept within 24 hrs. to reduce the negative effect on the cells.…”

Section: Discussionmentioning

confidence: 99%

A histological method for marine invertebrates

Kongthong

Charoenphon

Thaochan

et al. 2022

VIS

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This study aimed to increase knowledge about the structure and health assessment for evaluating histopathology as a biomarker of marine invertebrates, which must have high-quality histologic slides. Unfortunately, the accuracy of this method has been specifically modified for the marine invertebrates in Thailand; we demonstrated a modified method for permanent histological slides, including Xestospongia sp., Halobates hayanus, Amphibalanus amphitrite, and Alpheus sp. (n = 30) individual specimens for each species) from Libong island, Thailand, which was compared to the standard method. Optimization of tissue adapts and troubleshoots typical histological procedures, including sample collection, fixation with 10% Neutral Buffered Formalin at 2-4 °C for 24 hrs., decalcification, dehydration, clearing, embedding sectioning, and examining after staining under the modified procedures each time, which can be accomplished easily in six days. Compared to the standard method, this modified protocol showed the first report. It warranted high-quality slide scans, which are particularly ideal for the tissue, cell, and nuclei-specific features of marine invertebrates.

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Section: Discussionmentioning

confidence: 99%

A histological method for marine invertebrates

Kongthong

Charoenphon

Thaochan

et al. 2022

VIS

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“…Sections of the body wall were decalcified and dechitinized prior to processing using published protocols. 3 Cases were reviewed microscopically by 2 of the authors (C.F.M. and E.E.B.L.).…”

Section: Methodsmentioning

confidence: 99%

Histological Findings in Captive Madagascar Hissing Cockroaches (Gromphadorhina portentosa) and a Literature Review

Monahan

Bogan

LaDouceur³

2023

Vet Pathol

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Madagascar hissing cockroaches (MHC, Gromphadorhina portentosa) are members of the Blaberidae (giant cockroaches) family of the Insecta class. They are native to the African island of Madagascar where they live within leaf litter on the rainforest floor. Due to their large size, relative tameness, and general easy keeping, they have become popular in classrooms, zoological collections, museums, research laboratories, and as private exotic pets; however, descriptions of diseases of MHC in the literature are rare. The objective of this study is to describe and characterize postmortem histological findings in 18 captive MHC from a single zoological collection. In this retrospective study, 18 (4 females and 14 males) adult MHC necropsies were submitted to Northwest ZooPath between 2016 and 2020 for evaluation. The main organs with histological lesions were chitinous gut (foregut and/or hindgut; n = 17), tracheae ( n = 15), fat body ( n = 14), ventriculus (midgut) ( n = 13), body wall ( n = 12), Malpighian tubules ( n = 12), and hemolymphatic sinuses ( n = 12). All animals had inflammatory lesions affecting various organs. Inflammatory lesions typically consisted of aggregates of hemocytes with variable amounts of melanization and/or encapsulation. Bacterial, fungal, and parasitic infections were common and variably associated with hemocytic inflammation. Many of these organisms may represent symbiotic organisms of the MHC that cause opportunistic infections. This study contributes to the current knowledge of pathological findings and disease response of MHC and reviews diseases reported in multiple cockroach species.

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