DIAGNOSTIC USE OF THE PROTEIN C ACTIVATOR FROM<i>AGKISTRODON CONTORTRIX</i>

Gempeler-Messina, P.M.; Müller, C.

doi:10.1080/15569540600567362

Cited by 13 publications

(4 citation statements)

References 38 publications

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“…The activator used in commercial PC activity assays is derived from the Southern copperhead (Agkistrodon contortrix contortrix) and is known as Protac. 16 Activated PC cleaves and inactivates factors Va and VIIIa, which results in clotting time prolongation in the second assay stage. Clot-based PC activity assay may be based on activated partial thromboplastin time (aPTT), prothrombin time, or Russell viper venom time.…”

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confidence: 99%

Protein C Deficiency

Dinarvand

Moser

2019

Archives of Pathology &Amp; Laboratory Medicine

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Protein C (PC) deficiency is a heritable or acquired risk factor for thrombophilia, with presentations varying from asymptomatic to venous thromboembolism to neonatal purpura fulminans, a life-threatening disorder. Hereditary PC deficiency is caused by mutation in the PC ( PROC) gene located on chromosome 2q14.3. Heterozygous and acquired PC deficiencies are more common than homozygous deficiency. The recommended initial laboratory test measures PC activity using either clot-based or chromogenic methods. There are numerous potential interferences in PC activity testing that may result in either false-positive (falsely low activity) or false-negative (falsely normal or elevated activity) results. In the present review, we discuss common clinical presentations; laboratory testing, with a focus on potential assay interferences; treatment options; and prognosis in patients with PC deficiency.

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Protein C Deficiency

Dinarvand

Moser

2019

Archives of Pathology &Amp; Laboratory Medicine

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“…Инкубация протеина С с активатором из Agk. contortrix contortrix приводит в результате ограниченного протеолиза к превращению протеина С в его активную форму -активированный протеин С, который расщепляет специфический хромогенный пептидный субстрат pGlu-Pro-Arg-pNА (S-2366) с высвобождением свободного pNA, концентрация которого прямо пропорциональна концентрации протеина С в образце [4].…”

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Possibility of application of extracellular protease of micromycet Aspergillus ochraceus VKM F-4104D for determination of protein C content in human blood plasma

et al. 2018

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It was shown that the activator activity of protein C, determined in normal plasma using Aspergillus ochraceus protease, is comparable with the activity of commercial protease analogue from the South American copperhead venom (ProtacÒ). It was found that protease of A. ochraceus can be used to determine protein C in plasma with its reduced content similar to ProtacÒ. Comparison of the activator protein C activity of A. ochraceus protease and the commercial analogue showed some excess of the activator activity of the fungal preparation, which may make it a promising substitute for the snake activator in diagnostical kits for determining the protein C content in clinical laboratories.

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“…At present, proteolytic enzymes obtained from the venom of South American copperhead Agkistrodon contortrix contortrix are used for determination of protein C content in blood plasma and the diagnostic preparation Protac ® (Pentapharm, Switzerland) has been developed on this basis [3]. Protein C incubation with an activator from A. contortrix contortrix results in limited proteolysis and conversion of protein C into its active form, activated protein C, which cleaves the specific chromogenic peptide substrate pGlu-Pro-Arg-pNA (S-2366) with release of free pNA (p-nitroaniline) and the pNA concentration is directly proportional to the concentration of protein C in an analyzed sample [4].…”

Section: Introductionmentioning

confidence: 99%

Possibility of Application of Extracellular Protease of the Micromycetе Aspergillus ochraceus VKM F-4104D for Determination of the Protein C Content in Human Blood Plasma

Osmolovskiy

Orekhova

Kreyer

et al. 2018

Biochem. Moscow Suppl. Ser. B

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⎯The protein C activator activity, determined in normal plasma by using A. ochraceus protease is comparable with the activity of a commercial protease analogue from the South American copperhead venom (Protac ® ). As in the case of Protac ® , the A. ochraceus protease can be used for protein C determination in plasma with its reduced content. Comparison of the activator protein C activity of A. ochraceus protease and the commercial analogue showed some excess of the activator activity of the fungal preparation, which may be a promising substitute for the snake activator in diagnostical kits for determining the protein C content in clinical laboratories.

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DIAGNOSTIC USE OF THE PROTEIN C ACTIVATOR FROMAGKISTRODON CONTORTRIX

Cited by 13 publications

References 38 publications

Protein C Deficiency

Protein C Deficiency

Possibility of application of extracellular protease of micromycet Aspergillus ochraceus VKM F-4104D for determination of protein C content in human blood plasma

Possibility of Application of Extracellular Protease of the Micromycetе Aspergillus ochraceus VKM F-4104D for Determination of the Protein C Content in Human Blood Plasma

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