Diagnostic usefulness of molecular detection of Coxiella burnetii from blood of patients with suspected acute Q fever

Bae, Moonsuk; Jin, Choong Eun; Park, Joung Ha; Kim, Min Jae; Chong, Yong Pil; Lee, Sang‐Oh; Choi, Sang‐Ho; Kim, Yang Soo; Woo, Jun Hee; Shin, Yong Moon; Kim, Sung-Han

doi:10.1097/md.0000000000015724

Cited by 23 publications

(15 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Notably, the SMR sensor is capable of Q fever rapid detection within 10 min, except the extraction step of DNA from the plasma samples. Nevertheless, the sensor is faster than the conventional end-point PCR method for pathogen detection (>2 h) [28]. Therefore, we have shown that this SMR biosensor can be useful for pathogen detection in blood plasma samples.…”

Section: Resultsmentioning

confidence: 99%

“…The 35 blood plasma samples from 16 acute Q fever patients infected with Coxiella burnetii ( C. burnetii ) and 19 other febrile diseases were obtained using protocols approved by the Institutional Review Board of Asan Medical Center (2018–9023), Republic of Korea [28]. We also obtained the institutional approval and written consent of the patient.…”

Section: Methodsmentioning

confidence: 99%

“…SMR sensor technology, using extracted DNA from the blood plasma of infectious disease patients, shows that it is, however, possible to diagnose patients who are difficult to clinically diagnose quickly and in a real-time manner. Acute Q fever may progress to a persistent, intensive infection such as endocarditis if not initially treated, but it is difficult to diagnose because there are no distinct features that distinguish it from other febrile diseases [27,28]. In this study, we are developing a sensor based on SMR to detect the extracted DNA from 35 clinical samples (including 16 Q acute Q fever samples infected with Coxiella burnetii and 19 samples infected with other febrile diseases).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of Coxiella burnetii Using Silicon Microring Resonator in Patient Blood Plasma

et al. 2019

Self Cite

View full text Add to dashboard Cite

Blood plasma from patients is a powerful resource for diagnosing infectious disease due to it having many genetic materials as well as being relatively easy to obtain. Thus, various biosensors have been investigated for diagnosing diseases in blood plasma. However, there are no optimized and validated sensors for clinical use due to the low sensitivity, complexity, and difficulties of removing the inhibitors from plasma samples. In this study, we described a silicon microring resonator sensor used to detect Coxiella burnetii from the blood plasma of Q-fever patients in a label-free, real-time manner. Q-fever is an infectious disease caused by Coxiella burnetii via direct contact or inhalation aerosols. We validated this biosensor in the blood plasma of 35 clinical samples (including 16 Q fever samples infected with Coxiella burnetii and 19 samples infected with other febrile diseases. The biosensors are capable of rapid (10 min), highly sensitive (87.5%), and specific (89.5%) detection in plasma samples compared to the use of the conventional method.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of Coxiella burnetii Using Silicon Microring Resonator in Patient Blood Plasma

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…[35]. PCR is a useful detection tool for improving the accuracy of a diagnosis [21,36]; PCR detection of C. burnetii in blood was effective in diagnosing Q fever with a sensitivity of approximately 81% compared to indirect immuno uorescence assay (IFA) [37]. Therefore, UR-qPCR could be used for on-site conformational diagnosis of Q fever, for the prompt control of milk, blood, or serum samples.…”

Section: Discussionmentioning

confidence: 99%

Real-time PCR Biochip for On-Site Detection of Coxiella Burnetii in Ticks

Truong

Yun

Lim

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Q fever, a zoonosis caused by Coxiella burnetii, has adverse effects on public health. Ticks are the natural reservoirs of C. burnetii and they contribute to the transmission of the pathogen. A tool for rapid, sensitive, and accurate detection of C. burnetii from ticks is important for the prevention of Q fever. Methods: Ultra-rapid real-time PCR (UR-qPCR) as a chip-based real-time PCR system was developed for the detection of C. burnetii from ticks. The UR-qPCR system was established and evaluated for the rapidity, sensitivity, and specificity of C. burnetii detection. Results: C. burnetii was detected using UR-qPCR from 5,644 larval, nymphal, and adult ticks from 408 pools collected from livestock and epidemiologically linked environments in two provinces, Gangwon and Jeju, in Korea. Ticks from three species were identified; Haemaphysalis longicornis accounted for the highest number, present in 333 of 408 pools (81.62%), followed by Haemaphysalis flava in 62 pools (15.19%) and Ixodes nipponensis in 13 pools (3.19%). The rapidity and sensitivity of PCR detection was demonstrated with the sufficient amplification and detection of approximately 56 copies of C. burnetii DNA with only 20 min of PCR amplification. The kappa value for the diagnostic agreement between UR-qPCR and stationary qPCR was in perfect agreement (p = 1). PCR detection and sequencing indicated that C. burnetii was present in 5 of the 408 pools (1.23%), in which four pools contained H. longicornis and one pool contained H. flava. The infection rates of C. burnetii in the tick pools collected from Gangwon and Jeju Provinces were 1.70% and 0.58%, respectively. Phylogenetic analysis indicated a close relationship between the detected C. burnetii and those originated from goats, humans, and ticks in different countries, such as USA, France, Germany, and Serbia. Conclusions: The results of this study could be important for the prevention and control of Q fever in the two provinces. The UR-qPCR with its features of mobility, sensitivity, and rapidity is helpful for constructing early alert systems in the field for C. burnetii in ticks and for alleviating the transmission and economic damage due to Q fever.

show abstract

“…Diagnosis of Q fever is difficult due to the lack of distinct clinical features that distinguish it from other febrile diseases [5]. Currently, the diagnosis of Q fever mainly depends on the detection of antibodies or nucleic acids.…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid and quantitative detection of Coxiella burnetii phase I strains

et al. 2020

View full text Add to dashboard Cite

Background: Coxiella burnetii is an obligate intracellular Gram-negative bacterium that causes a zoonotic disease commonly called Q fever globally. In this study, an up-converting phosphor technology-based lateral flow (UPT-LF) assay was established for the rapid and specific detection of phase I strains of C. burnetii. Results: Specific monoclonal antibodies (10B5 and 10G7) against C. burnetii phase I strains were prepared and selected for use in the UPT-LF assay by the double-antibody-sandwich method. The detection sensitivity of the Coxiella-UPT-LF was 5 × 10 4 GE/ml for a purified C. burnetii phase I strain and 10 ng/ml for LPS of C. burnetii Nine Mile phase I (NMI). Good linearity was observed for C. burnetii phase I and NMI LPS quantification (R 2 ≥ 0.989). The UPT-LF assay also exhibited a high specificity to C. burnetii, without false-positive results even at 10 8 GE/ml of non-specific bacteria, and good inclusivity for detecting different phase I strains of C. burnetii. Moreover, the performance of the Coxiella-UPT-LF assay was further confirmed using experimentally and naturally infected samples. Conclusions: Our results indicate that Coxiella-UPT-LF is a sensitive and reliable method for rapid screening of C. burnetii, suitable for on-site detection in the field.

show abstract

Diagnostic usefulness of molecular detection of Coxiella burnetii from blood of patients with suspected acute Q fever

Cited by 23 publications

References 16 publications

Detection of Coxiella burnetii Using Silicon Microring Resonator in Patient Blood Plasma

Detection of Coxiella burnetii Using Silicon Microring Resonator in Patient Blood Plasma

Real-time PCR Biochip for On-Site Detection of Coxiella Burnetii in Ticks

Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid and quantitative detection of Coxiella burnetii phase I strains

Contact Info

Product

Resources

About