Diagnostic Utility of Satb2 in Metastatic Colorectal Carcinoma

Abstract

Objective: The purpose of this study was to evaluate the diagnostic utility of SATB2 in detecting CRC origin for patients presenting with metastatic carcinomas.
 Study Design: Cross sectional, comparative study.
 Place and Duration of Study: Study was conducted at department of Histopathology, Armed Forces Institute of Pathology, Rawalpindi from January 2019 to June 2019.
 Materials and Methods: Already diagnosed 68 cases of metastatic carcinoma of various origins with unknown primary were retrieved from tumour registry. Paraffin embedded blocks were taken and a panel of Immunohistochemistry was applied which includes CK7, CK20,CDX2 and SATB2. Positivity of SATB2 alone, and combination of SATB2 with CK 7,CK20 and CDX2 was evaluated.
 Results: Out of 68 metastatic adenocarcinoma cases 28(41%) had positive SATB2 expression and 38 (56%) were negative. All the positive SATB2 cases had 100 % (28/28) expression with CDX2, 93% (26/28) of the cases had expression with CK20 and 21% (6/28) of the cases showed expression of CK7. The sensitivity of SATB2 alone was 100% in metastatic colorectal carcinoma as compared to CK20 and CDX2. The sensitivity of SATB2 in combination with CDX2 (100%) and CK20 (93%)in comparison to the double combination of CK20 and CDX2(93 %). The sensitivity of SATB2 and CK20 combination was 93%, which was equal to CK20 and CDX2 combination.
 Conclusions: Our results show that SATB2 is a sensitive marker in the IHC panel for cases with suspected metastasis from colorectal region.

Diagnostic accuracy of SATB2 in identifying primary and metastatic colorectal carcinoma: a comparative immunohistochemical study

“…Considering the validity of the examined markers individually, this study achieved that SATB2 was more sensitive than CK20 but less sensitive than CDX2 in detection of CRC. However, SATB2 was more specific than CDX2 and CK20 similar to the findings of other reports [ 3 , 5 , 8 , 9 , 23 , 26 , 28 ]. Salim et al [ 28 ] reported that SATB2 and CDX2 had the same sensitivity, and theirs were higher than that of CK20.…”

“…However, SATB2 was more specific than CDX2 and CK20 similar to the findings of other reports [ 3 , 5 , 8 , 9 , 23 , 26 , 28 ]. Salim et al [ 28 ] reported that SATB2 and CDX2 had the same sensitivity, and theirs were higher than that of CK20. In contrast to Zhang et al [ 3 ] who concluded that CDX2 and CK20 were more sensitive than SATB2.…”

“…Similar results were detected by Dabir et al [ 5 ] and Salim et al [ 28 ] who reported that combining SATB2 and CDX2 had the best sensitivity and specificity in detection of CRC. Zhang et al study [ 3 ] reported that combined SATB2 and CDX2 showed better specificity, but in contrast, it revealed that the traditional combination of CDX2 with CK20 had a better sensitivity than other combinations.…”

The frequency of SATB2 and CDX2 expression in metastatic mucinous tumors of ovarian and colonic origin

“…In other words, there were no cases of positive SATB2 accompanied by negative CDX2. This finding is consistent with the study conducted by Salim et al, who reported that all positive SATB2 cases were also positive for CDX2 (9). Salim et al evaluated cases of already-diagnosed metastatic carcinoma with diverse origins and reported a high sensitivity of SATB2 expression for colorectal cancer, which aligns with the investigation conducted by Magnusson et al (10).…”

Immunohistochemical Evaluation of SATB2 Expression in Colorectal Carcinoma in Mosul City, Iraq

“…The SATB2 "special AT-rich sequence-binding protein 2", represents a transcription factor [9][10][11][12] that attaches to the DNA nuclear matrix attachment region and subsequently directs transcription and chromatin remodeling [3,4,[11][12][13]. The SATB2 gene is located on chromosome 2.…”