Diagnostic Value of Different Phenotype Circulating Tumor Cells in Hepatocellular Carcinoma

Cheng, Yuan; Luo, Lei; Ju-qiang, Zhang; Zhou, Mantian; Tang, Yujun; He, Guolin; Lu, Yishi; Zhong, Wen‐Zhao; Pan, Mingxin

doi:10.1007/s11605-018-04067-y

Cited by 39 publications

(38 citation statements)

References 38 publications

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“…We metastasis (19). Our team's previous research also confirmed that mesenchymal CTCs are more ideal early predictors of HCC recurrence (17,20). However, we found in the study that CTCs in the CTC-WBC clusters were almost all mixed CTCs.…”

Section: Discussionsupporting

confidence: 56%

“…CTCs are relatively safe and readily available "liquid biopsy" specimens (21,22). In fact, CTCs can serve as both an indicator of diagnosis and prognosis and provide molecular information to guide treatment decisions (17,22,23). Several studies have shown that CTCs are independent risk factors for HCC, and patients with higher CTC counts have a poorer prognosis (23,24).…”

Section: Discussionmentioning

confidence: 99%

“…The CanPatrol TM CTC analysis system (SurExam, China) was used to detect the number of CTCs and CTC-WBC clusters in 7.5 ml of whole samples of peripheral blood, similar to previous studies (17,18). RNA-ISH was used to detect the following target sequences: white blood cells were labeled with CD45 and visible as white fluorescence, epithelial cells were labeled with EpCAM and CK8/18/19 and visible as red fluorescence, and mesenchymal cells were labeled with vimentin/twist and visible as green fluorescence.…”

Section: Ctcs and Ctc-wbc Clusters Testmentioning

confidence: 99%

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Circulating Tumor-Cell-Associated White Blood Cell Clusters in Peripheral Blood Indicate Poor Prognosis in Patients With Hepatocellular Carcinoma

et al. 2020

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Aim: Circulating tumor cells (CTC) are a precursor to metastasis in several types of cancer and are occasionally found in the bloodstream in association with immune cells, such as white blood cells (WBCs). CTC-associated WBC (CTC-WBC) clusters can promote CTC appreciation and metastasis, suggesting that patients with CTC-WBC clusters found in the peripheral blood may have a worse prognosis. However, it is unclear whether CTC-WBC clusters are present in the peripheral blood of patients with hepatocellular carcinoma (HCC) and suggest a poor prognosis for HCC. Methods: We collected peripheral blood from 214 patients with HCC from January 2014 to December 2016. CanPatrol TM CTC analysis technology was used to isolate and count CTCs and CTC-WBC clusters in the patients' peripheral blood. Chi-squared analysis was used to calculate the correlation between the CTC-WBC clusters and clinicopathological characteristics. Kaplan-Meier survival analysis and Cox regression analysis were used to assess patient prognosis. Results: We used CanPatrol TM CTC analysis technology to count different types of CTCs and CTC-WBC clusters. The results showed that CTC-WBC clusters and tumor size (P = 0.001), tumor number (P = 0.005), portal vein tumor thrombus (P = 0.026), BCLC stage (P < 0.001), AFP level (P = 0.002), and total number of CTCs (P < 0.001) were statistically related. Cox regression analysis revealed that CTC-WBC clusters are an independent prognostic indicator of DFS (HR = 1.951, 95%CI:1.348-2.824, P < 0.001) and OS (HR = 3.026, 95%CI:1.906-4.802, P < 0.001) in HCC patients. Using Kaplan-Meier analysis, we found that positive CTC-WBC cluster patients had significantly shorter DFS and OS than patients with negative CTC-WBC (P < 0.001 and P < 0.001, respectively). Conclusions: CTC-WBC clusters in the peripheral blood are an independent predictor of DFS and OS, and their presence indicates poor prognosis in patients with HCC.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Ctcs and Ctc-wbc Clusters Testmentioning

confidence: 99%

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Circulating Tumor-Cell-Associated White Blood Cell Clusters in Peripheral Blood Indicate Poor Prognosis in Patients With Hepatocellular Carcinoma

et al. 2020

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“…It is reported that a presence or dominance of mesenchymal-like CTCs represented worse clinical outcomes for HCC patients due to earlier tumor relapse and metastasis [73,87] . The majority of these studies used the CanPatrol TM platform for CTC analysis [73,[88][89][90][91][92][93][94] . In a cohort of 113 HCC patients (65% BCLC 0/A) and 57 non-malignant liver diseases patients, the system presented a higher diagnostic value (AUC = 0.774, 95%CI: 0.704-0.834) of HCC than AFP [0.669 (AUC = 0.669, 95%CI: 0.587-0.750)].…”

Section: Early Detection and Prediction Of Tumor Progression In Hccmentioning

confidence: 99%

Detection of circulating tumor cells in hepatocellular carcinoma: applications in diagnosis, prognosis prediction and personalized treatment

Wang¹,

Yang²

2020

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Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide and is associated with poor clinical prognosis, which is mainly caused by tumor recurrence and metastasis. Circulating tumor cells (CTCs) are tumor cells shed into the bloodstream and regarded as the "seeds" of tumor recurrent or metastatic lesions. Over the past decade, the clinical value of CTC analysis has been extensively explored. CTC analysis is a representative form of liquid biopsy, offering a novel solution that can bypass the problems of invasive biopsy procedures, enabling comprehensive, non-invasive, and real-time disease monitoring. In HCC, CTC analysis has facilitated early detection and prognosis prediction, as well as treatment monitoring and therapeutic intervention guiding. In this review, we summarize available literature and provide an overview of CTC biology, detection technologies, and clinical applications in the diagnosis, prognosis prediction, and personalized treatment of HCC.

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“…In recent years, the diagnostic performance of blood-based liquid biopsies in HCC diagnosis has been widely studied. [ 8 – 10 ] However, these studies evaluated the sensitivities and specificities of a single liquid biopsy method. Besides, there were considerable discrepancies from inconsistent subjects, diverse detection techniques, and inconsistent results.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic performance of blood-based liquid biopsies in hepatocellular carcinoma

et al. 2020

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Background: Hepatocellular carcinoma (HCC) is an aggressive cancer associated with poor prognosis. Early diagnosis is crucial to improve its prognosis. Blood-based liquid biopsies are promising methods in detecting HCC. However, their accuracies have not been systematically assessed, so it is essential to conduct a meta-analysis to evaluate the diagnostic performance of blood-based liquid biopsies in detecting HCC. Methods: We will search PubMed, EMBASE, Cochrane Library, Web of Science, Medline, China National Knowledge Infrastructure(CNKI) for the relevant studies that assessed the diagnostic performance of blood-based liquid biopsies including circulating tumor cells(CTCs), circulating tumor DNA(ctDNA), and exosomes(EVs) in HCC patients from inception to September 2020. Two researchers will independently extract the data and use Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) to evaluate the quality of included literature. We will also conduct the pool diagnostic value, heterogeneity across studies and reporting bias. All the statistical analysis will be conducted by Stata V.15.0 and Meta-disc V.1.4. Results: This review will evaluate the pooled diagnostic value of blood-based liquid biopsies in HCC. Conclusion: This review will summarize the current published evidence of blood-based liquid biopsies in diagnosing HCC, which may provide a great opportunity for promotion and application of them. Open Science Framework(OSF) registration number: September 3, 2020. https://osf.io/9n4yz .

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Diagnostic Value of Different Phenotype Circulating Tumor Cells in Hepatocellular Carcinoma

Cited by 39 publications

References 38 publications

Circulating Tumor-Cell-Associated White Blood Cell Clusters in Peripheral Blood Indicate Poor Prognosis in Patients With Hepatocellular Carcinoma

Circulating Tumor-Cell-Associated White Blood Cell Clusters in Peripheral Blood Indicate Poor Prognosis in Patients With Hepatocellular Carcinoma

Detection of circulating tumor cells in hepatocellular carcinoma: applications in diagnosis, prognosis prediction and personalized treatment

Diagnostic performance of blood-based liquid biopsies in hepatocellular carcinoma

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