Diagnostic value of fecal cultures in dogs with chronic diarrhea

Werner, Melanie; Suchodolski, Jan S.; Lidbury, Jonathan A.; Steiner, Jörg M.; Hartmann, Katrin; Unterer, Stefan

doi:10.1111/jvim.15982

Cited by 14 publications

(16 citation statements)

References 55 publications

(117 reference statements)

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“…Hemolytic E coli were more frequently isolated from healthy dogs than dogs with diarrhea. This was in contrast with the molecular‐based Dysbiosis Index (see below), which was significantly higher, indicating dysbiosis in dogs with chronic diarrhea 48 …”

Section: Bacterial Culturementioning

confidence: 64%

“…Across all samples, bacterial culture results from all three laboratories did not reveal significant differences in microbiota between healthy dogs and dogs with chronic diarrhea. Interestingly, the laboratories reported dysbiosis more frequently in healthy dogs, and there was no agreement in the reported culture results between the three laboratories 48 . Hemolytic E coli were more frequently isolated from healthy dogs than dogs with diarrhea.…”

Section: Bacterial Culturementioning

confidence: 88%

“…Only a small percentage of bacterial species can be isolated from the feces of clinical patients, and these have been reported by commercial diagnostic laboratories. Unfortunately, because these bacteria are isolated from clinical patients; clinicians often erroneously consider them as pathogens (eg, E coli , C perfringens ) 48 . In a recent study, three aliquots of fecal samples from healthy dogs and dogs with chronic diarrhea were submitted in a blinded fashion to three veterinary reference laboratories for the evaluation of dysbiosis or the culture of pathogenic bacteria.…”

Section: Bacterial Culturementioning

confidence: 99%

“…In a recent study, three aliquots of fecal samples from healthy dogs and dogs with chronic diarrhea were submitted in a blinded fashion to three veterinary reference laboratories for the evaluation of dysbiosis or the culture of pathogenic bacteria. The authors ordered a so called “fecal bacterial culture profile” 48 . Across all samples, bacterial culture results from all three laboratories did not reveal significant differences in microbiota between healthy dogs and dogs with chronic diarrhea.…”

Section: Bacterial Culturementioning

confidence: 99%

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Analysis of the gut microbiome in dogs and cats

Suchodolski

2021

Veterinary Clinical Pathol

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The gut microbiome is an important immune and metabolic organ. Intestinal bacteria produce various metabolites that influence the health of the intestine and other organ systems, including kidney, brain, and heart. Changes in the microbiome in diseased states are termed dysbiosis. The concept of dysbiosis is constantly evolving and includes changes in microbiome diversity and/or structure and functional changes (eg, altered production of bacterial metabolites). Molecular tools are now the standard for microbiome analysis. Sequencing of microbial genes provides information about the bacteria present and their functional potential but lacks standardization and analytical validation of methods and consistency in the reporting of results. This makes it difficult to compare results across studies or for individual clinical patients. The Dysbiosis Index (DI) is a validated quantitative PCR assay for canine fecal samples that measures the abundance of seven important bacterial taxa and summarizes the results as one single number. Reference intervals are established for dogs, and the DI can be used to assess the microbiome in clinical patients over time and in response to therapy (eg, fecal microbiota transplantation). In situ hybridization or immunohistochemistry allows the identification of mucosa‐adherent and intracellular bacteria in animals with intestinal disease, especially granulomatous colitis. Future directions include the measurement of bacterial metabolites in feces or serum as markers for the appropriate function of the microbiome. This article summarizes different approaches to the analysis of gut microbiota and how they might be applicable to research studies and clinical practice in dogs and cats.

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Section: Bacterial Culturementioning

confidence: 64%

Section: Bacterial Culturementioning

confidence: 88%

Section: Bacterial Culturementioning

confidence: 99%

Section: Bacterial Culturementioning

confidence: 99%

See 2 more Smart Citations

Analysis of the gut microbiome in dogs and cats

Suchodolski

2021

Veterinary Clinical Pathol

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“…The abundance of Fusobacterium spp. is used in the calculation of the dysbiosis index (along with Faecalibacterium spp., Turicibacter spp., Streoptococcus spp., E. coli, Blautia spp., and Clostridium hiranonis), with reduced abundance (and higher dysbiosis index score) reported in dogs with chronic enteropathies (53,54). The dysbiosis index (DI) is a qPCR-based tool developed to assess fecal dysbiosis in dogs with chronic enteropathy, it utilizes bacterial taxa shown to be altered in studies of dogs with and without intestinal inflammation (53).…”

Section: Discussionmentioning

confidence: 99%

Longitudinal Survey of Fecal Microbiota in Healthy Dogs Administered a Commercial Probiotic

et al. 2021

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The aim of this longitudinal microbiome study was to investigate the effects of a commercially available veterinary synbiotic product (Blackmore's® Paw DigestiCare 60™) on the fecal microbiome of healthy dogs using 16S rRNA gene microbial profiling. Fifteen healthy, privately-owned dogs participated in a 2-week trial administration of the product. Fecal samples were collected at different time points, including baseline (prior to treatment), during administration and after discontinuation of product. Large intra- and inter-individual variation was observed throughout the study, but microbiome composition at higher phylogenetic levels, alpha and beta diversity were not significantly altered after 2 weeks of probiotic administration, suggesting an absence of probiotic impact on microbial diversity. Administration of the synbiotic preparation did, however, result in transient increases in probiotic species from Enterococacceae and Streptococacceae families as well as an increase in Fusobacteria; with the fecal microbiota partially reverting to its baseline state 3-weeks after cessation of probiotic administration.

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