Diagnostic value of molecular markers linked to the eyespot resistance gene Pch1 in wheat

Meyer, Nina; Lind, V.; Heindorf, Magdalena; Korzun, Viktor; Friedt, Wolfgang; Ordon, Frank

doi:10.1007/s10681-010-0266-0

Cited by 8 publications

(7 citation statements)

References 27 publications

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“…AB246917 is 96 and 91 % similar to Traes_7DL_DBBA5FD6C and Bradi29400 , respectively, and encodes the endopeptidase protein Ep-D1b. A number of the previous studies detected a complete linkage between Pch1 and either the isozyme marker (McMillin et al 1986; Santra et al 2006) or the STS marker (Burt and Nicholson 2011; Leonard et al 2008; Meyer et al 2011). In accordance with these studies, our data also did not identify any recombination event between Xorw1_D and Pch1 , not making it possible to refute the hypothesis that Ep-D1b could be a candidate for Pch1 .…”

Section: Discussionmentioning

confidence: 98%

The eyespot resistance genes Pch1 and Pch2 of wheat are not homoeoloci

et al. 2016

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Key message Phenotyping and mapping data reveal that chromosome intervals containing eyespot resistance genes Pch1 and Pch2 on 7D and 7A, respectively, do not overlap, and thus, these genes are not homoeloci. AbstractEyespot is a stem-base fungal disease of cereals growing in temperate regions. Two main resistances are currently available for use in wheat. Pch1 is a potent single major gene transferred to wheat from Aegilops ventricosa and located on the distal end of chromosome 7D. Pch2, a moderate resistance deriving from Cappelle Desprez, is located at the end of 7AL. The relative positions of Pch1 and Pch2 on 7D and 7A, respectively, suggest that they are homoeoloci. A single seed decent recombinant F7 population was used to refine the position of Pch2 on 7A. New markers designed to 7D also allowed the position of Pch1 to be further defined. We exploited the syntenic relationship between Brachypodium distachyon and wheat to develop 7A and 7D specific KASP markers tagging inter-varietal and interspecific SNPs and allow the comparison of the relative positions of Pch1 and Pch2 on 7D and 7A. Together, phenotyping and mapping data reveal that the intervals containing Pch1 and Pch2 do not overlap, and thus, they cannot be considered homoeloci. Using this information, we analysed two durum wheat lines carrying Pch1 on 7A to determine whether the Ae.ventricosa introgression extended into the region associated with Pch2. This identified that the introgression is distal to Pch2 on 7A, providing further evidence that the genes are not homoeoloci. However, it is feasible to use this material to pyramid Pch1 and Pch2 on 7A in a tetraploid background and also to increase the copy number of Pch1 in combination with Pch2 in a hexaploid background.

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Section: Discussionmentioning

confidence: 98%

The eyespot resistance genes Pch1 and Pch2 of wheat are not homoeoloci

et al. 2016

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“…Chapman et al (2008) also found that markers wmc14, barc97, and psr121 were closely linked with Pch1. Meyer et al (2011) reported that orw1, orw6, and cfd175 were the most suitable markers for marker assisted selection (MAS).…”

Section: Introductionmentioning

confidence: 99%

Mapping QTL for resistance to eyespot of wheat in Aegilops longissima

Sheng

See²,

Murray

2012

Theor Appl Genet

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Eyespot is an economically important disease of wheat caused by the soilborne fungi Oculimacula yallundae and O. acuformis. These pathogens infect and colonize the stem base, which results in lodging of diseased plants and reduced grain yield. Disease resistant cultivars are the most desirable control method, but resistance genes are limited in the wheat gene pool. Some accessions of the wheat wild relative Aegilops longissima are resistant to eyespot, but nothing is known about the genetic control of resistance. A recombinant inbred line population was developed from the cross PI 542196 (R) × PI 330486 (S) to map the resistance genes and better understand resistance in Ae. longissima. A genetic linkage map of the S(l) genome was constructed with 169 wheat microsatellite markers covering 1261.3 cM in 7 groups. F(5) lines (189) were tested for reaction to O. yallundae and four QTL were detected in chromosomes 1S(l), 3S(l), 5S(l), and 7S(l). These QTL explained 44 % of the total phenotypic variation in reaction to eyespot based on GUS scores and 63 % for visual disease ratings. These results demonstrate that genetic control of O. yallundae resistance in Ae. longissima is polygenic. This is the first report of multiple QTL conferring resistance to eyespot in Ae. longissima. Markers cfd6, wmc597, wmc415, and cfd2 are tightly linked to Q.Pch.wsu-1S ( l ), Q.Pch.wsu-3S ( l ), Q.Pch.wsu-5S ( l ), and Q.Pch.wsu-7S ( l ), respectively. These markers may be useful in marker-assisted selection for transferring resistance genes to wheat to increase the effectiveness of resistance and broaden the genetic diversity of eyespot resistance.

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“…Disease is caused by two closely related fungal species Oculimacula yallundae and O. acuformis which infect wheat (Triticum aestivum L., Triticum durum L.), barley (Hordeum vulgare L.), oats (Avena sativa L.) and rye (Secale cereale L.). In cases of severe infection, the disease causes lodging and premature ripening of grain, leading to yield losses of up to 40% (Meyer et al 2011).…”

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Yield reduction historically associated with the Aegilops ventricosa 7DV introgression is genetically and physically distinct from the eyespot resistance gene Pch1

et al. 2019

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Key message Yield penalty and increased grain protein content traits associated with Aegilops ventricosa 7D introgression have been mapped for the first time, and they are physically distinct from the eyespot resistance locus Pch1. Abstract Wheat wild relatives represent an important source of genetic variation, but introgression of agronomically relevant genes, such as for disease resistance, may lead to the simultaneous introduction of genetically linked deleterious traits. Pch1 is a dominant gene, conferring resistance to eyespot and was introgressed to wheat from Aegilops ventricosa as part of a large segment of the 7D V chromosome. This introgression has been associated with a significant yield reduction and a concomitant increase in grain protein content. In this study, we evaluated both traits and their relationship to the location of the Pch1 gene. We found that both QTLs were clearly distinct from the Pch1 gene, being located on a different linkage group to Pch1. In addition, we found that the QTL for increased grain protein content was strong and consistent across field trials, whereas the yield penalty QTL was unstable and environmentally dependent. The yield and grain protein content QTLs were genetically linked and located in the same linkage group. This finding is due in part to the small size of the population, and to the restricted recombination between wheat 7D and Ae. ventricosa 7D v chromosomes. Although recombination in this interval is rare, it does occur. A recombinant line containing Pch1 and 7D_KASP6, the marker associated with increase in grain protein content, but not Xwmc221, the marker associated with the yield penalty effect, was identified.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Diagnostic value of molecular markers linked to the eyespot resistance gene Pch1 in wheat

Cited by 8 publications

References 27 publications

The eyespot resistance genes Pch1 and Pch2 of wheat are not homoeoloci

The eyespot resistance genes Pch1 and Pch2 of wheat are not homoeoloci

Mapping QTL for resistance to eyespot of wheat in Aegilops longissima

Yield reduction historically associated with the Aegilops ventricosa 7DV introgression is genetically and physically distinct from the eyespot resistance gene Pch1

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