Diagnostic value of PCR for detection of borrelia burgdorferi DNA in clinical specimens from patients with erythema migrans and lyme neuroborreliosis

Lebech, Anne‐Mette; Hansen, Klaus; Brandrup, Flemming; Clemmensen, Ole; Halkier-Sørensen, L

doi:10.1054/xd.2000.7179

Cited by 42 publications

(48 citation statements)

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“…B. burgdorferi DNA has been detected in synovial fluid samples from 85% of patients [46]. However, the sensitivity of PCR determinations in CSF from patients with neuroborreliosis has been much lower (<40% in patients with acute neuroborreliosis) [47,48]. In skin biopsies from erythema migrans lesions, PCR sensitivity varies from 25% to 90% and is similar to culture [43,49].…”

Section: Culturementioning

confidence: 97%

Lyme Disease: A Review

Marques

2010

Curr Allergy Asthma Rep

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Lyme disease is the most common vector-borne illness in the United States and is also endemic in Europe and Asia. It is caused by the spirochete Borrelia burgdorferi and transmitted by the bite of the Ixodes (deer) tick. It occurs most frequently during spring and summer and may involve the skin, nervous system, heart, and joints. This article reviews the pathogenesis, epidemiology, clinical manifestations, diagnosis, treatment, and prevention of Lyme disease.

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Section: Culturementioning

confidence: 97%

Lyme Disease: A Review

Marques

2010

Curr Allergy Asthma Rep

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“…Cultivation is a laborious and tedious method, and both procedures are invasive and would not detect possible residual borrelial infection of other tissues. Culture or polymerase chain reaction analyses of blood or urine are less valuable and not established for routine diagnosis [8]. In clinical practice, serum anti- Bb sl antibody analyses would be most convenient, but for enzyme-linked immunosorbent assay (ELISA) testing, it has been shown recently that repeated examinations of anti- Bb sl antibodies after antibiotic therapy of patients with EM do not provide additional information about the efficacy of treatment to the clinician [9].…”

Section: Introductionmentioning

confidence: 99%

Immunoblot Analysis of the Seroreactivity to Recombinant <i>Borrelia burgdorferi </i>sensu lato Antigens, Including VlsE, in the Long-Term Course of Treated Patients with Erythema Migrans

et al. 2008

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Objective: We evaluated whether immunoblotting is capable of substantiating the posttreatment clinical assessment of patients with erythema migrans (EM), the hallmark of early Lyme borreliosis. Methods: In 50 patients, seroreactivity to different antigens of Borrelia burgdorferi sensu lato was analyzed by a recombinant immunoblot test (IB) in consecutive serum samples from a minimum follow-up period of 1 year. Antigens in the IgG test were decorin-binding protein A, internal fragment of p41 (p41i), outer surface protein C (OspC), p39, variable major protein-like sequence expressed (VlsE), p58 and p100; those in the IgM test were p41i, OspC and p39. Immune responses were correlated with clinical and treatment-related parameters. Results: Positive IB results were found in 50% before, in 57% directly after therapy and in 44% by the end of the follow-up for the IgG class, and in 36, 43 and 12% for the IgM class. In acute and convalescence phase sera, VlsE was most immunogenic on IgG testing (60 and 70%), and p41i (46 and 57%) and OspC (40 and 57%) for the IgM class. By the end of the follow-up, only the anti-p41i IgM response was significantly decreased to 24%. Conclusions: No correlation was found between IB results and treatment-related parameters. Thus, immunoblotting does not add to the clinical assessment of EM patients after treatment.

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“…In Lyme arthritis, excellent laboratories can detect spirochete DNA by polymerase chain reaction (PCR) in most instances [7]. In Lyme meningitis, culture is usually positive in no more than 10%; PCR positivity is variable but rarely much greater than that [8,9]. Biopsies of human nervous system tissue, or of brain or nerve, in the only animal model of neuroborreliosis (the rhesus macaque monkey) have never demonstrated convincing evidence of spirochetes, their antigens, or their DNA [10][11][12].…”

Section: Pathophysiologymentioning

confidence: 99%

Neurologic Manifestations of Lyme Disease

Halperin

2011

Curr Infect Dis Rep

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Borrelia burgdorferi infection, the tick-borne spirochetosis known as Lyme disease or Lyme borreliosis, involves the nervous system (neuroborreliosis) in 10% to 15% of patients. Common manifestations include lymphocytic meningitis, cranial neuritis, mononeuropathy multiplex, and painful radiculoneuritis. Rare patients develop inflammation in the brain or spinal cord. Regardless of the form of involvement, neuroborreliosis can be microbiologically cured in virtually all patients using standard 2- to 4-week antimicrobial regimens. Oral regimens appear to be as effective as parenteral ones in most instances. Although patients ill with Lyme disease may have concomitant cognitive or memory difficulty, these symptoms are not specific to neuroborreliosis and, when present in isolation, should not be viewed as suggestive of this diagnosis. When present as part of Lyme disease, they do not require additional or different treatment.

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Diagnostic value of PCR for detection of borrelia burgdorferi DNA in clinical specimens from patients with erythema migrans and lyme neuroborreliosis

Cited by 42 publications

References 0 publications

Lyme Disease: A Review

Lyme Disease: A Review

Immunoblot Analysis of the Seroreactivity to Recombinant <i>Borrelia burgdorferi </i>sensu lato Antigens, Including VlsE, in the Long-Term Course of Treated Patients with Erythema Migrans

Neurologic Manifestations of Lyme Disease

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