Diagnostic value of serum creatine kinase and lactate dehydrogenase isoenzyme determinations for monitoring early cardiac damage in rats

Preus, Marianette; Bhargava, A.S.; Khater, Abd El Rahman; Günzel, P

doi:10.1016/0378-4274(88)90081-1

Cited by 47 publications

(25 citation statements)

References 10 publications

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“…1). This finding is consistent with previous results on the early myocardial damage induced by isoproterenol [1,9]. There were no differences in the degree of the change among the dose levels, unlike the results on the clinicopathological parameters described below.…”

supporting

confidence: 93%

“…In addition, different parameters are sometimes employed in toxicity studies, so knowledge regarding such correlation would be valuable information for evaluating the toxicity across toxicity studies (i.e., bridging data between toxicity studies). We have therefore investigated the correlation among clinicopathological parameters of myocardial damage in rats treated with isoproterenol, which is a typical compound for inducing myocardial damage [1,9].…”

mentioning

confidence: 99%

“…Preus et al [9] reported the usefulness of LDH-1, LDH-2, and CK-MB, while various other authors reported troponin I and/or troponin T as markers for myocardial damage in rats [1,2,4,5,8,12]. Recently, the International Life Science Institute (ILSI) / Health Environmental Science Institute (HESI) have undertaken a comprehensive study focusing on the influence of methodological differences regarding troponin I detection in diluted samples of serum from rats treated with isoproterenol.…”

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confidence: 99%

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Correlation among Clinicopathological Parameters of Myocardial Damage in Rats Treated with Isoproterenol

et al. 2007

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Abstract:The correlation among clinicopathological parameters of myocardial damage was investigated in rats administered a single subcutaneous dose of isoproterenol at 0 (saline), 0.04, 0.4 and 4 mg/kg. Total lactate dehydrogenase (LDH), creatine kinase (CK), and their isoenzymes (LDH-1, LDH-2 and CK-MB), as well as troponin I and tropinin T, were measured 4 h after the administration of the drug. Troponin I was determined by a chemiluminescence method using Bayer Centaur and DPC Immulyze, as well as by ELISA. Troponin T was assayed semi-quantitatively using Trop T Sensitive. A high correlation was found among LDH isoenzymes, troponin I (Centaur) and troponin T. The present result provides a baseline for interpreting changes in the different parameters of myocardial damage assayed by different methods in toxicity studies.

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confidence: 93%

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confidence: 99%

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confidence: 99%

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Correlation among Clinicopathological Parameters of Myocardial Damage in Rats Treated with Isoproterenol

et al. 2007

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“…1H, the release rates of LDH, an index of tissue damage (40), were similar during the prestress period in all four groups. Addition of 10 M epinephrine induced a progressive increase in the LDH release, which reached a plateau at 30 min for Wistar rat hearts but continued to rise in SHR hearts at all afterload pressures.…”

Section: Effect Of An Acute Adrenergic Stress In Working Shr Vs Wistmentioning

confidence: 70%

Fatty acid oxidation and its impact on response of spontaneously hypertensive rat hearts to an adrenergic stress: benefits of a medium-chain fatty acid

Labarthe

Khairallah²,

Bouchard³

et al. 2005

American Journal of Physiology-Heart and Circulatory Physiology

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Des Rosiers. Fatty acid oxidation and its impact on response of spontaneously hypertensive rat hearts to an adrenergic stress: benefits of a medium-chain fatty acid. Am J Physiol Heart Circ Physiol 288: H1425-H1436, 2005. First published November 18, 2004; doi:10.1152/ajpheart.00722.2004.-The spontaneously hypertensive rat (SHR) is a model of cardiomyopathy characterized by a restricted use of exogenous long-chain fatty acid (LCFA) for energy production. The aims of the present study were to document the functional and metabolic response of the SHR heart under conditions of increased energy demand and the effects of a medium-chain fatty acid (MCFA; octanoate) supplementation in this situation. Hearts were perfused ex vivo in a working mode with physiological concentrations of substrates and hormones and subjected to an adrenergic stimulation (epinephrine, 10 M).13 C-labeled substrates were used to assess substrate selection for energy production. Compared with control Wistar rat hearts, SHR hearts showed an impaired response to the adrenergic stimulation as reflected by 1) a smaller increase in contractility and developed pressure, 2) a faster decline in the aortic flow, and 3) greater cardiac tissue damage (lactate dehydrogenase release: 1,577 Ϯ 118 vs. 825 Ϯ 44 mU/min, P Ͻ 0.01). At the metabolic level, SHR hearts presented 1) a reduced exogenous LCFA contribution to the citric acid cycle flux (16 Ϯ 1 vs. 44 Ϯ 4%, P Ͻ 0.001) and an enhanced contribution of endogenous substrates (20 Ϯ 4 vs. 1 Ϯ 4%, P Ͻ 0.01); and 2) an increased lactate production from glycolysis, with a greater lactate-to-pyruvate production ratio. Addition of 0.2 mM octanoate reduced lactate dehydrogenase release (1,145 Ϯ 155 vs. 1,890 Ϯ 89 mU/min, P Ͻ 0.001) and increased exogenous fatty acid contribution to energy metabolism (23.7 Ϯ 1.3 vs. 15.8 Ϯ 0.8%, P Ͻ 0.01), which was accompanied by an equivalent decrease in unlabeled endogenous substrate contribution, possibly triglycerides (11.6 Ϯ 1.5 vs. 19.0 Ϯ 1.2%, P Ͻ 0.01). Taken altogether, these results demonstrate that the SHR heart shows an impaired capacity to withstand an acute adrenergic stress, which can be improved by increasing the contribution of exogenous fatty acid oxidation to energy production by MCFA supplementation. isolated working rat heart perfusion; citric acid cycle;13 C mass isotopomer analysis; fatty acid translocase/CD36; epinephrine ACCUMULATING EVIDENCE INDICATES that alterations in substrate selection for energy production can affect the heart's susceptibility to injury as well as the development of hypertrophy and heart failure (2,8,19,47,57). Specifically, interventions favoring carbohydrate over fatty acid (FA) oxidation were repeatedly shown to enhance the function of the ischemicreperfused heart (27, 51) or the failing heart (51). This notion has led to the development of new drugs, such as ranolazine, oxfenicine, and trimetazidine, whose effects are attributed to inhibition of FA oxidation (47, 51). What remains to be clarified, however, is how much can FA oxid...

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“…The determinations of creatine kinase 1 ) and lactate dehydrogenase 1 ) isoenzymes in the serum have been quite useful in the diagnosis of specific tissue damage in heart, skeletal muscle and liver disorders in humans. However, these methods have not been so fully explored in a variety of animal species, specially in toxicological studies.…”

Section: Introductionmentioning

confidence: 99%

Serum Isoenzyme Pattern of Creatine Kinase and Lactate Dehydrogenase in Various Animal Species

Preus¹,

Karsten²,

Bhargava³

1989

Clinical Chemistry and Laboratory Medicine

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Summary:The creatine kinase and lactate^dehydrogenase isoenzyme pattera were determined in the serum of normal and untreated rats, rabbits, dogs, monkeys and pigs. The relative distribution of all isoenzymes in the serum and an electrophoretic pattern for each animal species are presented. The isoenzyme serum pattern showed a great Variation between the species. The diagnostic value of serum creatine kinase isoenzyme MB and lactate dehydrogenase isoenzymes l and 2 in predicting cardiac lesions in different animal species is briefly discussed.

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Diagnostic value of serum creatine kinase and lactate dehydrogenase isoenzyme determinations for monitoring early cardiac damage in rats

Cited by 47 publications

References 10 publications

Correlation among Clinicopathological Parameters of Myocardial Damage in Rats Treated with Isoproterenol

Correlation among Clinicopathological Parameters of Myocardial Damage in Rats Treated with Isoproterenol

Fatty acid oxidation and its impact on response of spontaneously hypertensive rat hearts to an adrenergic stress: benefits of a medium-chain fatty acid

Serum Isoenzyme Pattern of Creatine Kinase and Lactate Dehydrogenase in Various Animal Species

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