Diagnostic Value of Thrombocyte Aggregates in Sarcoidosis by Flow Cytometric Analysis

Abstract

Sarcoidosis is a granulomatous disease characterized by a multisystemic, hyperimmune response that can frequently involve the lung and lymphatic system, but has the potential to involve all organs in the body. A type IV hypersensitivity reaction, in which T cells play a role, is seen with cellular immune response. In addition to clinical and radiological findings, non-caseous granulation seen in biopsy material is used in its diagnosis. In addition, it has been shown that the CD4/CD8 ratio in the bronchoalveolar lavage fluid can be used together with other parameters in the diagnosis of the disease. In this study, it was aimed to evaluate the role of the presence of aggregates in monocyte-neutrophil cell lines in the diagnosis of the disease and the usability of the CD4/CD8 ratio in peripheral blood in patients diagnosed with sarcoidosis. 15 patients with sarcoidosis and 15 healthy individuals were included in the study. Analysis of the samples was carried out in a flow cytometry device (BD, FacsCalibur) using dyes suitable for lymphocyte surface markers CD4-8 and platelet surface markers CD41a-61-14. In the study, no difference was found between CD4/CD8 ratios. In the monocytic series, there was a significant difference between the patient and control groups in terms of CD14-CD61(p<0.001), CD41a(p=0.045) and CD61(p<0.001) values. In addition, a significant difference was found in CD41a (p=0.035) and CD61 (p<0.001) values in neutrophils when comparing patient and control groups (p<0.05). By using the data of the study, further studies are planned that are thought to contribute to the parameters that can be used in the diagnosis of sarcoidosis.