Diagnostic Values of Surface‐Enhanced Laser Desorption/Ionization Technology for Screening of Habitual Drinkers

Abstract

The results of this study clearly indicate that the 5.9 kDa peak is a promising novel biological marker for moderate alcohol consumption.

Increased Concentrations of Apo A-I and Apo A-II Fragments in the Serum of Patients With Hepatocellular Carcinoma by Magnetic Beads–Assisted MALDI-TOF Mass Spectrometry

“…21,22 We used SELDI technology to generate comparative protein profiles of consecutive serum samples obtained during abstinence from alcoholic patients and found some potential biomarker for excessive alcohol consumption. 23,24 Using this technique, several protein peaks leading to differentiation of patients with HCC from patients with cirrhosis alone have been discovered. [25][26][27][28] Although SELDI-TOF MS can rapidly analyze many samples at a time, it has several drawbacks including high cost, difficulty in further identifying proteins, and limitation in separating small peptides.…”

Application of proteomic technologies to discover and identify biomarkers for excessive alcohol consumption: A review

“…The next question was how these novel markers perform as screening tools when applied to subjects with moderate alcohol consumption in the general population. We determined the diagnostic values of the novel markers in screening male habitual drinkers with moderate alcohol consumption (hereafter habitual drinkers) in the general population as compared with two conventional markers, GGT and CDT [5].…”

“…In this article, we summarize our recent comprehensive proteomic studies to discover and identify novel biomarkers for excessive alcohol consumption [4][5][6].…”

Establishment and application of a high-quality comparative analysis strategy for the discovery and small-scale validation of low-abundance biomarker peptides in serum based on an optimized novel peptide extraction method

“…Some of the LMW biomarkers discovered using these methods are indicative of disease onset [1][2][3][4][5][6][7] , suggesting that native peptides may represent an unexplored archive of histological information. However, only a small proportion of peptides present in serum/plasma at concentrations above 100 ng/mL can be analyzed using conventional approaches.…”

“…Quantitative analysis of the proteins/peptides in serum/plasma samples is frequently employed in research aimed at discovering disease-specific biomarkers useful for the early detection of disease, for predicting drug susceptibility, and for evaluating prognosis [6][7][8][9] . The low-molecularweight (LMW) protein/peptide component of the serum/ plasma includes members of several physiologically important classes, such as cytokines, chemokines, and peptide hormones, along with proteolytic fragments of larger proteins 10,11) , including those generated by cancer-specific exopeptidases 12) .…”