Diagnostic yield of real-time polymerase chain reaction in the diagnosis of intrapartum maternal rectovaginal colonization by group B Streptococcus : a systematic review with meta-analysis

Feuerschuette, Otto Henrique May; Silveira, Sheila Koettker; Cancelier, Ana Carolina Labor; Silva, Rosemeri Maurici da; Trevisol, Daisson José; Pereira, Jefferson Ricardo

doi:10.1016/j.diagmicrobio.2018.01.013

Cited by 25 publications

(22 citation statements)

References 37 publications

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“…A recent meta-analysis showed a sensitivity of 93.7% (95% CI 92.1%-95.3%) and specificity of 97.6% (95% CI 96.9%-98.0%) for intralabor RT-PCR. 16 However, the proportion of invalid results affects the sensitivity and specificity of PCR-based GBS detection in clinical practice.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…3,7,[14][15][16] However, the disadvantages of RT-PCR compared with culture at present are the higher costs per screening and an inevitable percentage of invalid results, usually around 10% but reported to be up to 19% in the first run of the PCR sample. 16 We studied the rate of GBS colonization and the sensitivity and specificity of late-pregnancy enriched culture with increasing screening-to-labor interval compared with intrapartum GBS status as determined by both RT-PCR and enriched culture. 16 We studied the rate of GBS colonization and the sensitivity and specificity of late-pregnancy enriched culture with increasing screening-to-labor interval compared with intrapartum GBS status as determined by both RT-PCR and enriched culture.…”

Section: Introductionmentioning

confidence: 99%

“…GBS detection by RT-PCR has high sensitivity (83%-98.5%) and short turnaround time, making it suitable for intralabor use, and it is therefore recommended for GBS detection by the European Consensus conference. 3,7,[14][15][16] However, the disadvantages of RT-PCR compared with culture at present are the higher costs per screening and an inevitable percentage of invalid results, usually around 10% but reported to be up to 19% in the first run of the PCR sample. 15,17 In a recent systematic review with meta-analysis by Feuerschuette et al, a conclusion was made that GBS screening with RT-PCR is a promising tool, but more studies are needed to define its cost-benefit ratio.…”

Section: Introductionmentioning

confidence: 99%

“…15,17 In a recent systematic review with meta-analysis by Feuerschuette et al, a conclusion was made that GBS screening with RT-PCR is a promising tool, but more studies are needed to define its cost-benefit ratio. 16 We studied the rate of GBS colonization and the sensitivity and specificity of late-pregnancy enriched culture with increasing screening-to-labor interval compared with intrapartum GBS status as determined by both RT-PCR and enriched culture.…”

Section: Introductionmentioning

confidence: 99%

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The effect of screening‐to‐labor interval on the sensitivity of late‐pregnancy culture in the prediction of group B streptococcus colonization at labor: A prospective multicenter cohort study

Virranniemi

Raudaskoski

Haapsamo

et al. 2019

Acta Obstet Gynecol Scand

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Introduction:The aim of this study was to evaluate the effect of increasing screening-to-labor interval on the performance of group B streptococcus (GBS) screening by late-pregnancy enriched culture compared with intrapartum real-time polymerase chain reaction (RT-PCR). Material and methods: Group B streptococcus colonization was determined in 2624women with singleton pregnancies by culture at 35-37 weeks of gestation and at the beginning of labor by culture and RT-PCR from recto-vaginal swab samples. Results:Group B streptococcus colonization rates were 29.0% in late-pregnancy culture, 29.7% in intrapartum culture and 28.2% in intrapartum PCR. Intrapartum culture was used as a reference, the late-pregnancy culture had an overall sensitivity of 89.2% (95% CI 88.0%-90.4%) and specificity of 96.5% (95% CI 95.8%-97.2%), and intrapartum PCR had sensitivity of 91.5% (95% CI 90.4%-92.6%) and specificity of 98.5% (95% CI 98.0%-99.0%). However, up to 4 weeks after screening, the sensitivity of late-pregnancy culture was equivalent to or higher than that of RT-PCR. The RT-PCR was invalid in 0.9% of the women. Between late-pregnancy screening and labor, GBS colonization changed from negative to positive in 3.2% and from positive to negative in 2.5% of the women. Conclusions:The late-pregnancy enriched culture and intrapartum RT-PCR have comparable sensitivities in the detection of GBS when culture screening is conducted no more than 4 weeks before labor. Late-pregnancy culture sampling should be postponed to at least 37 weeks of gestation. K E Y W O R D S enriched culture, Group B streptococcus, intrapartum RT-PCR, late-pregnancy Group B streptococcus screening, screening | 495 VIRRANNIEMI Et Al.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of screening‐to‐labor interval on the sensitivity of late‐pregnancy culture in the prediction of group B streptococcus colonization at labor: A prospective multicenter cohort study

Virranniemi

Raudaskoski

Haapsamo

et al. 2019

Acta Obstet Gynecol Scand

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Genital Cultures

Filkins,

Garner

2023

ClinMicroNow

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Routine genital cultures enable detection of nonfastidious aerobic bacteria and are only indicated for some clinical presentations of genital infections. When a routine genital culture is ordered, potentially clinically significant bacteria should be evaluated using an indication specific approach but often include Enterobacterales , Pseudomonas aeruginosa , S. aureus , and Streptococcus pyogenes . For certain pathogens, such as Chlamydia trachomatis , N. gonorrhoeae , Haemophilus ducreyi , or S. agalactiae (group B streptococcus (GBS) for prenatal screening), cultures are ordered specifically for those pathogens, with proper collection and selection media or cell culture. For diagnosis of GBS disease in nonpregnant adults and in neonates, routine culture of the symptomatic body site and routine cultures collected based on clinical risk assessment will detect this pathogen, along with the other potential pathogens that can be isolated from those cultures.

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Infektionen in der Schwangerschaft und bei Geburt

Enders,

Kagan

2023

Die Geburtshilfe

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Diagnostic yield of real-time polymerase chain reaction in the diagnosis of intrapartum maternal rectovaginal colonization by group B Streptococcus : a systematic review with meta-analysis

Cited by 25 publications

References 37 publications

The effect of screening‐to‐labor interval on the sensitivity of late‐pregnancy culture in the prediction of group B streptococcus colonization at labor: A prospective multicenter cohort study

The effect of screening‐to‐labor interval on the sensitivity of late‐pregnancy culture in the prediction of group B streptococcus colonization at labor: A prospective multicenter cohort study

Genital Cultures

Infektionen in der Schwangerschaft und bei Geburt

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