Diagnóstico de las parasitosis intestinales mediante detección de coproantígenos

Rhipicephalus Boophilus microplus cattle tick is a scourge for livestock production. The infestations produced by this pathogen are incompletely contained by chemical treatments, with the associated environmental pollution risks. Vaccination against cattle ticks has emerged as a feasible and environmentally friendly strategy to control tick-borne diseases. In this setting, Gavac® vaccine has proven effective in decreasing cattle tick populations through antibody responses against the tick Bm86 antigen, as part of an Integrated Control Program. However, animal vaccination programs require easy and ready-to-use screening tests to follow up the immune response in vaccinated animals under field conditions. This study reports the evaluation HeberFast® Line Gavac, a lateral flow immunochromatographic system for the rapid detection of anti Bm86 antibodies in vaccinated cattle. The system was tested on 598 serum samples taken from immunized animals, arranged in three groups according to their anti-Bm86 antibody response in ELISA (209 high, 150 medium or 239 low and 100 samples from non-immunized animals. The HeberFast® Line Gavac system was assessed for sensitivity, specificity, and concordance against the ELISA reference test. Consistency was evaluated among production batches and inter-analyst reading-independent consistency at two moments: ten minutes after completing the test and after strip drying. The system showed high sensitivity (81.6%, 82.2%, and 81%), specificity (96.7, 94.6, and 93.3%), and agreement with the ELISA reference test (75%; 74%, and 71%) for high, medium and low anti-Bm86 sera, respectively. The effectiveness of the diagnosis was 87.6; 87.1; 85.9 for high, medium, and low antibody titers, respectively. Consistency among production batches and analysts was documented, and no significant differences between evaluation times were found. These results indicate that HeberFast® Line Gavac is a valuable tool for the serological surveillance of Gavac vaccinated cattle.

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“…and Cryptosporidium spp. in feces, 58-97.2 % sensitivity and 99-100 % specificity were reported 17,18 .…”

Section: Discussionmentioning

confidence: 97%

Assessing HeberFast® Line Gavac, a lateral flow immunochromatographic system for the rapid detection of anti-Bm86 antibodies in Gavac vaccinated cattle

Vargas-Hernández

Lorenzo

Perez

et al. 2021

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“…This reality is changing in areas with different economic and climatic characteristics, such as Spain, where numbers are increasing. The reasons for this include migration from endemic countries, increased international travel, consumption of fresh food and fostering/adoption of children from these areas, more consumption of organically grown food and the climate crisis [ 22 , 23 , 24 , 25 , 26 , 27 ]. In the present study, 49.5% of the participants had intestinal parasitism, a high number compared to other studies which reported between 10.7% and 28.0% [ 27 , 32 , 35 , 36 , 37 , 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is common for nutrition studies to be carried out with the variables described above, such as anthropometric, dietary and lifestyle values, leaving aside the possible causes of infectious and immunological origin that may be associated with these states of malnutrition, such as intestinal parasitism. These infections are an important child health problem that has historically affected mainly low-income countries, but is becoming more frequent in high-income countries, such as Spain, due to demographic, climatic and behavioural changes [ 22 , 23 , 24 , 25 , 26 , 27 ]. There are some intestinal parasite species associated with an altered nutritional status, such as the unicellulars Giardia duodenalis and Cryptosporidium spp.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Health Status of Spanish Schoolchildren Based on Nutrimetry, Lifestyle and Intestinal Parasites

et al. 2023

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Malnutrition in Spanish schoolchildren, and its relationship with lifestyles, has been studied, but Nutrimetry (a nutritional status indicator), and data on intestinal parasitism and its risk factors, have never before been taken into account. A total of 206 children aged 3–11 years, from two schools in the Valencian Community, participated. Demographic characteristics, diet, lifestyles, behavioural habits and anthropometric (weight, height) and coproparasitological data were collected. Nutrimetry was used to analyse nutritional status. Statistical analyses were performed to ascertain associations between lifestyle, selected parasite species and nutritional status. Multivariate logistic regression analysis was used to assess the strength of the association of the suspected risk factors with the presence of intestinal parasitism. The prevalence of overweight was 32.6%. A total of 43.9% had a high adherence to the Mediterranean Diet, for which mean daily intake was 2428.7 kcal. Intestinal parasitism was identified in 49.5% of the children (Giardia duodenalis: 28.6%). The source of drinking water was found to be a risk factor for intestinal parasitism. No positive association between the variables analysed and nutritional status could be confirmed. Nutrimetry is a good indicator for a complete analysis of nutritional status. It highlights the prevalence of overweight. Intestinal parasitism was identified in almost half of the participants and is a variable that should not be underestimated.

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“…La detección de los coccidios se realiza tradicionalmente mediante la demostración microscópica de los ooquistes tras tinciones especiales, como la de Kinyoun o de auramina-rodamina. Como alternativas válidas existen en el mercado diversas técnicas de detección de antígenos, como la IFD, el ELISA o las técnicas ICT, que han mostrado valores de especificidad y de sensibilidad superiores a los de la observación microscópica 7,11,13 . Sin embargo, ninguno de los métodos mencionados permite la identificación de especies, para lo cual es necesario recurrir a técnicas de PCR.…”

Section: Coccidiosunclassified

Diagnóstico rápido de las infecciones del tracto gastrointestinal por parásitos, virus y bacterias

Balsalobre-Arenas

Alarcón-Cavero

2017

Enfermedades Infecciosas y Microbiología Clínica

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Rapid diagnostic techniques are valuable tools in the diagnosis of gastrointestinal infections, especially for the detection of some microorganisms and in certain groups of patients. While antigen detection techniques are widely used in Clinical Microbiology laboratories, for the diagnosis of viruses, some parasites and some bacteria, molecular techniques are routinely used only for some pathogens (such as Clostridium difficile). However, molecular techniques are constantly evolving, and they allow a rapid diagnosis for an increasing number of pathogens, with high sensitivity and specificity. In addition, they are also able to detect virulence factors or resistance mechanisms. Syndromic surveillance systems, which detect different pathogens simultaneously, are very promising because they enable the most frequent pathogens to be diagnosed in a few hours and they can be very useful in certain patients. For the diagnosis of Helicobacter pylori infection, molecular techniques are able to detect bacteria and its resistance to clarithromycin and levofloxacin, allowing the most appropriate treatment to be selected for each patient when bacterial culture is not possible.

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Diagnóstico de las parasitosis intestinales mediante detección de coproantígenos

Cited by 8 publications

References 50 publications

Assessing HeberFast® Line Gavac, a lateral flow immunochromatographic system for the rapid detection of anti-Bm86 antibodies in Gavac vaccinated cattle

Assessing HeberFast® Line Gavac, a lateral flow immunochromatographic system for the rapid detection of anti-Bm86 antibodies in Gavac vaccinated cattle

Assessment of the Health Status of Spanish Schoolchildren Based on Nutrimetry, Lifestyle and Intestinal Parasites

Diagnóstico rápido de las infecciones del tracto gastrointestinal por parásitos, virus y bacterias

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