Diagnóstico de leptospirosis humana: evaluación de la aglutinación macroscópica en diferentes etapas de la enfermedad

Vanasco, Norma Bibiana; Schmeling, María Fernanda; Chiani, Yosena; Lottersberger, Javier; Tarabla, Héctor D.

doi:10.1590/s0036-36342012000500010

Cited by 8 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As previouslymentioned for Lepto-LF, IgM-ELISA and SATR also lack sensitivity and yielded LR-> 0.5 in the early acute phase of the disease showing poor suitability for screening diagnosis. In previous studies, we have already reported low sensitivity values for SATR (Se = 71.1%) and IgM-ELISA (Se = 76.6%) in the acute phase of the disease, which was de ned as 0-9 dpo [17,31]. However, in the present study, we observed a notable variation in sensitivity from 0-5 dpo to 6-10 dpo, raising their values to Se > 95%.…”

Section: Discussioncontrasting

confidence: 59%

“…The antigen employed was based on the Hardjoprajtino strain (serogroup Sejroe, serovar Hardjo). We have previously used other serovars for the development of other tests, and they showed no signi cant differences between them [29,30] likely due to the genus-speci c characteristics of lysed antigens [23]. It is worth noting that this is the same antigen used in IgM-ELISA for leptospirosis screening in Argentina, demonstrating high diagnostic accuracy [31].…”

Section: Discussionmentioning

confidence: 94%

“…Regarding leptospirosis screening, the slide agglutination test using temperatureresistant antigen (SATR) and IgM-ELISA are used [13]. SATR is economic, rapid and does not require equipment or expensive reagents; however, it is not well-accepted due to its poor inter-operator reproducibility [7,17]. IgM-ELISA provides objective results in only 2-4 hours [12] and is simpler than con rmatory methods, but it still requires some technical expertise and expensive equipment [11].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of a New Accurate Lateral Flow Immunoassay for Enhanced Diagnosis of Human Leptospirosis

Pujato,

Gimenez,

Peretti

et al. 2024

Preprint

View full text Add to dashboard Cite

Purpose Leptospirosis is a global neglected disease. Current diagnostic methods are technically complex, expensive, and often inaccurate, primarily confined to specialized laboratories. New simple and accurate tests are mandatory to decentralize and improve diagnosis. Here, we introduced a new lateral flow immunoassay (Lepto-LF) for human leptospirosis. Methods We conducted a double-blinded assay using 104 serum samples from patients with or without leptospirosis, diagnosed according to the standard algorithm. Diagnostic performance of Lepto-LF was estimated across various days from onset of symptoms (dpo), comparing it with the current methods: enzyme-linked immunosorbent assay (IgM-ELISA) and the slide agglutination test using temperature-resistant antigen (SATR). Results Lepto-LF exhibited perfect diagnostic performance with a Youden´s index J = 1, from the acute phase starting at 6 dpo. IgM-ELISA gave slightly lower accuracy (J = 0.91) with 95.5% sensitivity (Se) and specificity (Sp), while SATR showed very poor diagnostic yield (J = 0.41, Se = 95.5%, Sp = 45.5%). Performances remained similar in the convalescence phase of the disease (> 10 dpo). Conclusion Lepto-LF proved to be a reliable test with performance similar to current screening methods but with significant advantages. Due to its simplicity and speed, it can be used in low/medium-complexity labs, providing rapid results for early disease detection and timely treatment during the acute phase when antibiotics are highly effective. Additionally, Lepto-LF can serve as a confirmatory test, especially where the standard MAT is unavailable. Lepto-LF holds promise for remote areas and vulnerable environments, promoting decentralized diagnosis and ensuring equal access nationwide.

show abstract

Section: Discussioncontrasting

confidence: 59%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of a New Accurate Lateral Flow Immunoassay for Enhanced Diagnosis of Human Leptospirosis

Pujato,

Gimenez,

Peretti

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…In addition, Ricardo et al [ 25 ] found that there was a high proportion of people that inhabited riverside communities in the region with risky practices for leptospirosis and scarce knowledge about the disease. Finally, biases in national epidemiological databases have been previously reported [ 26 , 27 ]. Therefore, our aims were twofold: (1) to identify the spatial distribution of the suitability for human leptospirosis and (2) to find distinct combinations of determinants that may lead to the occurrence of the disease across the urban agglomeration of Santa Fe.…”

Section: Introductionmentioning

confidence: 99%

Determining the spatial distribution of environmental and socio-economic suitability for human leptospirosis in the face of limited epidemiological data

et al. 2022

View full text Add to dashboard Cite

Background Leptospirosis is among the leading zoonotic causes of morbidity and mortality worldwide. Knowledge about spatial patterns of diseases and their underlying processes have the potential to guide intervention efforts. However, leptospirosis is often an underreported and misdiagnosed disease and consequently, spatial patterns of the disease remain unclear. In the absence of accurate epidemiological data in the urban agglomeration of Santa Fe, we used a knowledge-based index and cluster analysis to identify spatial patterns of environmental and socioeconomic suitability for the disease and potential underlying processes that shape them. Methods We geocoded human leptospirosis cases derived from the Argentinian surveillance system during the period 2010 to 2019. Environmental and socioeconomic databases were obtained from satellite images and publicly available platforms on the web. Two sets of human leptospirosis determinants were considered according to the level of their support by the literature and expert knowledge. We used the Zonation algorithm to build a knowledge-based index and a clustering approach to identify distinct potential sets of determinants. Spatial similarity and correlations between index, clusters, and incidence rates were evaluated. Results We were able to geocode 56.36% of the human leptospirosis cases reported in the national epidemiological database. The knowledge-based index showed the suitability for human leptospirosis in the UA Santa Fe increased from downtown areas of the largest cities towards peri-urban and suburban areas. Cluster analysis revealed downtown areas were characterized by higher levels of socioeconomic conditions. Peri-urban and suburban areas encompassed two clusters which differed in terms of environmental determinants. The highest incidence rates overlapped areas with the highest suitability scores, the strength of association was low though (CSc r = 0.21, P < 0.001 and ESc r = 0.19, P < 0.001). Conclusions We present a method to analyze the environmental and socioeconomic suitability for human leptospirosis based on literature and expert knowledge. The methodology can be thought as an evolutive and perfectible scheme as more studies are performed in the area and novel information regarding determinants of the disease become available. Our approach can be a valuable tool for decision-makers since it can serve as a baseline to plan intervention measures.

show abstract

“…Un mal diagnóstico puede llevar a un manejo inadecuado y causar la muerte del paciente, principalmente en infecciones con escasa sospecha clínica como la rickettsiosis o leptospirosis. En México y otros países existe un subdiagnóstico de leptospirosis debido a su mimetismo clínico con dengue y otras enfermedades febriles 4 . Respecto a dengue, en los estados mexicanos que reportaron su presencia, la circulación es continua 5 .…”

Section: Introductionunclassified

Prevalencia de dengue y leptospirosis en pacientes febriles en un hospital general de especialidades, México

Cen

Blum-Domínguez

Núñez-Oreza

et al. 2018

Rev. chil. infectol.

View full text Add to dashboard Cite

Prevalence of dengue and leptospira in febrile patients of the general hospital of specialities in Mexico Among the infectious diseases characterized by a febrile picture are: dengue, leptospirosis, rickettsiosis and salmonellosis, among others. The objective of this study was to identify IgM antibodies against dengue and Leptospira in febrile patientes. The seropositivity for IgM antibodies to dengue was 34%; 26.3% for women and 7.6% for men, without differences significant (p = 0.181). The seropositivity for IgM antibodies to Leptospira was 3.2%, being found only in women. Positive serology for both dengue and Leptospira was 1%. The serovars detected were Pomona and Canicola by the microagglutination technique. It was possible to identify the unsuspected presence of leptospirosis and dengue in atypical months for the latter, an important fact to consider the study of serology in the differential diagnosis of febrile diseases.

show abstract

Diagnóstico de leptospirosis humana: evaluación de la aglutinación macroscópica en diferentes etapas de la enfermedad

Cited by 8 publications

References 22 publications

Development of a New Accurate Lateral Flow Immunoassay for Enhanced Diagnosis of Human Leptospirosis

Development of a New Accurate Lateral Flow Immunoassay for Enhanced Diagnosis of Human Leptospirosis

Determining the spatial distribution of environmental and socio-economic suitability for human leptospirosis in the face of limited epidemiological data

Prevalencia de dengue y leptospirosis en pacientes febriles en un hospital general de especialidades, México

Contact Info

Product

Resources

About