Diagnóstico diferencial de filariasis importada mediante técnicas moleculares (2006-2009)

Jiménez, Maribel; González, Luís Miguel; Bailo, Begoña; Blanco, A I de Cos; García, Luz; Pérez-González, Francisco; Fuentes, Isabel; Gárate, Teresa

doi:10.1016/j.eimc.2011.06.012

Cited by 11 publications

(4 citation statements)

References 21 publications

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“…112 Researchers from Spain used a nested PCR for Loa loa on more than 500 blood samples and demonstrated a higher sensitivity of molecular diagnosis in comparison with Knott's concentration technique. 113 The third patient, a French man who had worked in Gabon for several months, developed ocular loiasis 8 years after his stay in Africa. Interestingly, clinical manifestations coincided with surgery due to an aortic aneurysm.…”

Section: Discussionmentioning

confidence: 99%

Imported Loa loa filariasis: three cases and a review of cases reported in non-endemic countries in the past 25 years

Antinori

Schifanella

Million

et al. 2012

International Journal of Infectious Diseases

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The aim of this study was to highlight the increasing chance of Western physicians encountering patients (both immigrants and expatriates/travelers) seeking help for loiasis. Methods: We describe three cases of imported loiasis observed at two hospitals in Italy and France, and present a review of all previously published cases in the medical literature in the last 25 years . The search was performed using PubMed and Scopus databases using the terms ''Loa loa'' AND ''loiasis''. Results: We reviewed 101 cases of imported loiasis of which 61 (60.4%) were reported from Europe and 31 (30.7%) from the USA. Seventy-five percent of infestations were acquired in three countries: Cameroon, Nigeria, and Gabon. Overall, peripheral blood microfilariae were detected in 61.4% of patients, eosinophilia in 82.1%, eye worm migration in 53.5%, and Calabar swellings in 41.6%. However, Calabar swellings and eosinophilia were more common among expatriates/travelers, whereas African immigrants were more likely to have microfilaremia. Eye worm migration was observed in a similar proportion in the two groups. Only 35 patients (including the three described here) underwent clinical follow-up for a median period of 10.5 months (range 1-84 months); clinical relapse occurred in three of these patients and persistence or reappearance of blood microfilaria in another two. Conclusions: Due to increasing travel and the migration of people from the endemic countries of West Africa to Europe and the USA, we speculate on the possible emergence of loiasis. Western physicians should be aware of the typical (eye worm migration and Calabar swellings) as well as unusual clinical presentations.

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Section: Discussionmentioning

confidence: 99%

Imported Loa loa filariasis: three cases and a review of cases reported in non-endemic countries in the past 25 years

Antinori

Schifanella

Million

et al. 2012

International Journal of Infectious Diseases

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“…Genomic DNA from the whole blood EDTA tubes using the QIAamp® DNA Blood Kit (Qiagen, Hilden, Germany). Nested PCR for L. Loa detection was adapted from Jimenéz et al, (2011) . Briefly, a first PCR, targeted a 475 bp region of the ribosomal Internal Transcribed Space (ITS) DNA region common to different nematode species was performed and visualized in 2.0% agarose gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

Clinical, serological and DNA testing in Bengo Province, Angola further reveals low filarial endemicity and opportunities for disease elimination

Paulo

Brito

Van-Dunem

et al. 2020

Parasite Epidemiology and Control

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The prevalence of Loa loa, Onchocerca volvulus and Wuchereria bancrofti infections in an under-surveyed area of Bengo Province, Angola, was determined by surveying 22 communities with a combination of clinical, serological and DNA diagnostics. Additional information was collected on participants' duration of residency, access to mass drug administration, knowledge of insect vectors and use of bednets. A total of 1616 individuals (38.1% male: 61.9% female), with an average age of 43 years, were examined. For L. loa, 6.2% ( n = 100/16616) individuals were found to have eyeworm, based on the rapid assessment procedure for loiasis (RAPLOA) surveys, and 11.5% ( n =178/1543) based on nested PCR analyses of venous blood. L. loa prevalences in long-term residents (>10 years) and older individuals (>60 years) were significantly higher, and older men with eyeworm were better informed about Chrysops vectors. For O. volvulus , 4.7% ( n = 74/1567) individuals were found to be positive by enzyme-linked immunosorbent assay (Ov 16 ELISA), with only three individuals reporting to have ever taken ivermectin. For W. bancrofti, no infections were found using the antigen-based immunochromatographic test (ICT) and real-time PCR analysis; however, 27 individuals presented with lymphatic filariasis (LF) related clinical conditions (lymphoedema = 11, hydrocoele = 14, both = 2). Just under half (45.5%) of the participants owned a bednet, with the majority (71.1%) sleeping under it the night before. Our approach of using combination diagnostics reveals the age-prevalence of loiasis alongside low endemicity of onchocerciasis and LF. Future research foci should be on identifying opportunities for more cost-effective ways to eliminate onchocerciasis and to develop innovative surveillance modalities for clinical LF for individual disease management and disability prevention.

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“…La gran mayoría de los estudios en epidemiología molecular que incluyen de forma explícita a población inmigrante en España se centraron en dos patógenos, la bacteria M. tuberculosis y el Virus de la Inmunodeficiencia Humana (VIH-1) que, en conjunto, superaron los 2/3 del total de estudios (36/50). De las publicaciones restantes cabe destacar la presencia de 3 estudios sobre Staphylococcus aureus y 3 sobre el Virus de la Hepatitis B (VBH), mientras que en 2 estudios se analizaron patógenos eucariotas, el díptero Phlebotomus sergenti 14 y los nematodos que producen filariasis 15 .…”

Section: Materials Y Métodosunclassified

“…El único estudio con el virus de la rubeola 30 se centró en el análisis de un brote y el correspondiente a las filariasis 15 en el desarrollo de un diagnóstico molecular para el nematodo causante de la infección.…”

Section: Figura 2 Distribución De Los Trabajos Publicados Sobre Epideunclassified

Estudios de epidemiología molecular en población inmigrante en España

Gónzález-Candelas¹,

Bracho²,

Comas³

et al. 2014

Rev. Esp. Salud Publica

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RESUMENFundamentos: La epidemiología molecular es una nueva disciplina que permite la integración de la información sobre la variabilidad genéti-ca de patógenos infecciosos con su difusión en la población y subgrupos de la misma incluyendo, por ejemplo, las mutaciones de resistencia a antibióticos y antivirales. El objetivo es conocer qué posibles diferencias existe en las características genéticas de los agentes infecciosos que afectan a las poblaciones inmigrante y autóctoctona en España.. Métodos:Se revisaron artículos originales publicados entre 1998-2013, con las palabras clave "epidemiología molecular", "tipado molecular", "secuenciación", "inmigrante", "España".Resultados: De un total de 267 artículos identificados inicialmente, 50 pasaron los diferentes filtros establecidos. De ellos, 36 analizan las infecciones por Mycobacterium tuberculosis y VIH, seguidos de los que analizan infecciones por Staphylococcus aureus (3) y el Virus de la Hepatitis B (3).Conclusiones: Los objetivos principales de estos trabajos fueron el tipado del patógeno y la determinación de la frecuencia de mutaciones de resistencia. Los estudios más frecuentes correspondieron a cohortes retrospectivas, seguidos por los estudios ecológicos y los ensayos clínicos. En general los estudios son descriptivos y su ámbito por el tipo y tamaño de muestra es bastante restringido. En varios se determina que las cepas o variantes del patógeno encontradas en inmigrantes tienen su origen más probable en sus países de origen, si bien otros también ponen de manifiesto la transmisión desde la población autóctona a la inmigrante. ABSTRACT Molecular Epidemiology Studies on the Immigrant Population in SpainBackground: Molecular epidemiology is a new scientific discipline which allows to integrate information on the genetic variation of infectious pathogens with their diffusion in a population and its subgroups including, for instance, resistance mutations to antibiotics and antiretrovirals. We present the results of an analysis of scientific publications that analyze the health status of the immigrant population in Spain from a molecular epidemiology perspective.

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Diagnóstico diferencial de filariasis importada mediante técnicas moleculares (2006-2009)

Cited by 11 publications

References 21 publications

Imported Loa loa filariasis: three cases and a review of cases reported in non-endemic countries in the past 25 years

Imported Loa loa filariasis: three cases and a review of cases reported in non-endemic countries in the past 25 years

Clinical, serological and DNA testing in Bengo Province, Angola further reveals low filarial endemicity and opportunities for disease elimination

Estudios de epidemiología molecular en población inmigrante en España

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