Diagnóstico microbiológico de la infección por Clostridium difficile

Alcalá-Hernández, Luis; Mena-Ribas, Ana; Niubó-Bosh, Jordi; Marín-Arriaza, Mercedes

doi:10.1016/j.eimc.2015.09.004

Cited by 16 publications

(10 citation statements)

References 21 publications

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“…Samples must be sent as soon as possible to the microbiology laboratory. In general, it is recommended to preserve samples at 2-8ºC the first 48-72 hours or frozen at -60 to -80ºC if samples will not be processed within the following 72 hours [19].…”

Section: Question 1 When Should CDI Be Suspected In Patients Older Tmentioning

confidence: 99%

“…This is the case of CDI due to ribotype 027 strains that are usually more severe, with a higher transmission and recurrence rate than CDI caused by other ribotypes. In case of suspicion of an outbreak due to this ribotype, it is recommended to perform a rapid molecular test that specifically detects this ribotype [19,119].…”

Section: Question 6 What Combination Of Laboratory Tests Is Most Cosmentioning

confidence: 99%

“…Overall performance including turnaround time of a GDH-based algorithm depends on the secondary tests used to follow up a positive GDH result. GDH detection followed by a NAAT is considered a two-step algorithm and has approximately a 90% sensitivity, and specificity higher than 99% [19]. A three-step algorithm detects toxins A and/or B between the GDH detection and NAATs, reducing almost by 50% the number of molecular tests needed [19].…”

Section: Question 7 When and How To Perform Antimicrobial Susceptibimentioning

confidence: 99%

“…GDH detection followed by a NAAT is considered a two-step algorithm and has approximately a 90% sensitivity, and specificity higher than 99% [19]. A three-step algorithm detects toxins A and/or B between the GDH detection and NAATs, reducing almost by 50% the number of molecular tests needed [19]. However, in the recent update of CDI guidelines published recently by IDSA and SHEA the recommendation is to use a stool toxin test as part of a multistep algorithm (i.e., GDH plus toxin; GDH plus toxin arbitrated by NAATs; or NAATs plus toxin) [21].…”

Section: Question 7 When and How To Perform Antimicrobial Susceptibimentioning

confidence: 99%

“…Accurate diagnosis of CDI is suboptimal and laboratory methods to diagnose the disease can be misleading due to the development in the last years of multiple tests with different sensitivities, specificities and targets (bacterium, toxins, cell membrane enzyme, toxin genes) [8,10,18,19].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Recommendations for the diagnosis and treatment of Clostridioides difficile infection: An official clinical practice guideline of the Spanish Society of Chemotherapy (SEQ), Spaish Society of Internal Medicine (SEMI) and the working group of Postoperative Infection of the Spanish Society of Anesthesia and Reanimation (SEDAR)

Bouza¹,

Aguado²,

Alcalá³

et al. 2020

Rev Esp Quimioter

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ned leukocytosis, even in the absence of classical risk factors. With a few exceptions, a single stool sample is sufficient for diagnosis, which can be sent to the laboratory with or without transportation media for enteropathogenic bacteria. In the absence of diarrhoea, rectal swabs may be valid. The microbiology laboratory should include C. difficile among the pathogens routinely searched in patients with diarrhoea. Laboratory tests in different order and sequence schemes include GDH detection, presence of toxins, molecular tests and toxigenic culture. Immediate determination of sensitivity to drugs such as vancomycin, metronidazole or fidaxomycin is not required. The evolution of toxin persistence is not a suitable test for follow up. Laboratory diagnosis of CDI should be rapid and results reported and interpreted to clinicians immediately. In addition to the basic support of all diarrheic episodes, CDI treatment requires the suppression of antiperistaltic

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Section: Question 1 When Should CDI Be Suspected In Patients Older Tmentioning

confidence: 99%

Section: Question 6 What Combination Of Laboratory Tests Is Most Cosmentioning

confidence: 99%

Section: Question 7 When and How To Perform Antimicrobial Susceptibimentioning

confidence: 99%

Section: Question 7 When and How To Perform Antimicrobial Susceptibimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Recommendations for the diagnosis and treatment of Clostridioides difficile infection: An official clinical practice guideline of the Spanish Society of Chemotherapy (SEQ), Spaish Society of Internal Medicine (SEMI) and the working group of Postoperative Infection of the Spanish Society of Anesthesia and Reanimation (SEDAR)

Bouza¹,

Aguado²,

Alcalá³

et al. 2020

Rev Esp Quimioter

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Lateral flow immunoassay for simultaneous detection of C. difficile, MRSA, and K. pneumoniae

Rubio-Monterde,

Rivas,

Gallegos

et al. 2024

Microchim Acta

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Mainly performed within a rapid diagnostic tests company, a lateral flow (LF) system using gold nanoparticles (AuNPs) as transducers is presented able to detect three bacteria of interest, of relevance for antimicrobial resistance (AMR): Clostridioides difficile, methicillin-resistant Staphylococcus aureus (MRSA), and Klebsiella pneumoniae, with a limit of detection of 25 ng/mL of glutamate dehydrogenase (GDH) for C. difficile, 36 ng/mL of penicillin-binding protein 2a (PBP2a) for MRSA, and 4 × 106 CFU/mL for K. pneumoniae. The system showed good results with bacteria culture samples, is user-friendly, and suitable for rapid testing, as the results are obtained within 15 min. Graphical Abstract

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Enfermedad recurrente por Clostridium difficile ribotipo 027

Rodríguez-Villodres

Praena

Vidal-Acuña

et al. 2016

Enfermedades Infecciosas y Microbiología Clínica

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Diagnóstico microbiológico de la infección por Clostridium difficile

Cited by 16 publications

References 21 publications

Lateral flow immunoassay for simultaneous detection of C. difficile, MRSA, and K. pneumoniae

Enfermedad recurrente por Clostridium difficile ribotipo 027

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