Diagnóstico post mortem de Fasciola hepatica en bovinos faenados en la planta de beneficio de Sogamoso (Boyacá, Colombia)

Ortiz-Pineda, Melissa-Camila; Archila-Barrera, Omar Alexander; Bulla-Castañeda, Diana María; Díaz-Anaya, Adriana María; Forero, Julio César Giraldo; García-Corredor, Diego; Pulido-Medellín, Martín Orlando

doi:10.15381/rivep.v32i5.21341

Cited by 2 publications

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“…Regarding the sex of the evaluated animals, studies have reported a greater prevalence of the disease in female animals [14,30,37], which is consistent with data found in San Miguel de Sema and Ubaté. However, Ortiz-Pineda et al [13] reported that male animals showed greater seroprevalence, consistent with data found in Chiquinquirá. Furthermore, a statistically significant association was found between the sex of the cattle and the presence of antibodies against fasciolosis in Ubaté, which is in agreement with data reported by Chaouadi et al [37], Pinilla et al [14], and Utrera-Quintana et al [8], where female animals presented a higher risk of parasitic infection, establishing the female sex as a risk factor for the disease.…”

Section: Discussionsupporting

confidence: 80%

“…Nevertheless, at the national level, the presence of antibodies was established in 5.6% of cattle evaluated in Sabana de Torres (Santander) [34]; 52.4% in animals from the municipalities of El Encino, Duitama, and Belén [35]; 37.8% in 16 municipalities in Colombia [14]; and 13.7% in cattle slaughtered at the Sogamoso (Boyacá) processing plant [13]; these rates were lower than those found in Chiquinquirá, Ubaté, and San Miguel de Sema. The different seroprevalence rates found in our study may be due to the number of bovines sampled, the type of study performed, the ELISA kit used, and the environmental conditions of the geographical location where the study was conducted, because it has been reported that the infection involves a suitable intermediate host in appropriate environments, such as proximity to streams and springs, an optimal temperature between 10°C and 25°C, and sufficient humidity for the disease to occur in ruminants [32,36].…”

Section: Discussionmentioning

confidence: 99%

“…In Colombia, the existence of endemic zones of Fasciola spp. is recognized, including the departments of Boyacá, Antioquia, and Cundinamarca [10][11][12][13][14][15], and the annual economic losses are approximately $1927 million pesos (US$479,962) [16]. To control this disease, conventional diagnosis has been conducted based on the direct method of detection and egg count in fecal samples after the prepatent period of the parasitosis, where sedimentation techniques improve sensitivity but are not sufficient for early identification of fasciolosis, due to the difficulty of the interpretation of results due to factors such as the long prepatent period and irregular egg shedding [17,18].…”

Section: Introductionmentioning

confidence: 99%

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Seroprevalence and risk factors of bovine fasciolosis in the municipalities of Colombia

et al. 2023

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Background and Aim: Bovine fasciolosis is a reemerging neglected disease with a worldwide distribution caused by the trematode Fasciola spp., which parasitize various hosts. Bovine fasciolosis is responsible for large economic losses in the bovine livestock sector. This study aimed to estimate the seroprevalence and risk factors of bovine fasciolosis in the municipalities of Colombia. Materials and Methods: This was a descriptive cross-sectional study with simple random sampling conducted on 1140 cattle from the municipalities of Chiquinquir´, San Miguel de Sema, and Ubaté for a duration of 3 months. Serum samples were processed using the commercial Fasciola hepatica Antibody Test Kit IDEXX® Fasciolosis Verification (IDEXX, United States), which identified immunoglobulin G antibodies for gf2 antigen purified from Fasciola extracts. The f2 antigen is extremely immunogenic and highly specific for F. hepatica. An epidemiological survey was performed to record variables related to the sampled animals and herd management practices. Data were processed using the statistical program Epi Info® (Centers for Disease Control and Prevention; Atlanta, Georgia). The prevalence ratio was estimated to evaluate the association between fasciolosis and the hypothesized causal factors and the significance of this association using Pearson’s Chi-square test. Finally, a logistic regression model was developed. Results: The overall seroprevalence was 72.3%. The seroprevalence was 83.9% (323/385) in Chiquinquirá, 68.17% (257/377) in Ubaté, and 64.55% (244/378) in San Miguel de Sema. The seroprevalence was higher in male animals in Chiquinquirá and in female animals in San Miguel de Sema and Ubaté. Similarly, sex showed a statistically significant association with disease prevalence in Ubaté. The highest prevalence was found in cattle aged >2 years. The Holstein breed showed maximum seroprevalence in Chiquinquirá (p ≤ 0.05) and San Miguel de Sema, whereas crossbreed showed higher seroprevalence in Ubaté. Similarly, in Chiquinquirá, the association between the seroprevalence of fasciolosis and the presence of other species was statistically significant (95% confidence interval [CI]: 0.9601–3.4944; p = 0.0448). In Ubaté, the disease presentation was also associated with pasture rental (95% CI: 0.4047–1.0023; p = 0.003) and attendance to livestock expositions (95% CI: 0.2313–1.0636; p = 0.044). However, in San Miguel de Sema, water from the stream showed a statistically significant association with disease presentation (95% CI: 0.5209–1.0985; p = 0.00649785). Female sex and diarrhea occurrence were considered risk factors for fasciolosis. Conclusion: A high seroprevalence of antibodies to Fasciola spp. was detected in cattle in the study municipalities, indicating a high parasite distribution in these areas. Female sex and diarrhea were established as risk factors associated with fasciolosis in Ubaté and San Miguel de Sema, respectively. Further, research is necessary to establish prevention and control programs against parasitosis. Keywords: bovine, fasciolosis, risk factors, seroprevalence.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Seroprevalence and risk factors of bovine fasciolosis in the municipalities of Colombia

et al. 2023

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“…The parasite is endemic in four recognized regions: Nariño, Cundiboyacense highlands, Santander, Norte de Santander highlands, and highlands of the west of Antioquia (19). The prevalence of F. hepatica varies across these areas, with values ranging from 9.5 to 30.9% (20)(21)(22)(23)(24)(25). However, knowledge of the parasite's genetic diversity and intrapopulation structure in the country is limited.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Fasciola hepatica in endemic regions of Colombia

García-Corredor¹,

Alvarado²,

Pulido-Medellín³

et al. 2023

Front. Vet. Sci.

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Fasciola hepatica is a zoonotic trematode that affects a wide range of hosts, including cattle, sheep, and goats. The economic impact of the parasite on the cattle industry is significant, with high losses reported worldwide. While its impact on human health was previously underestimated, recent years have seen a rise in fascioliasis cases, leading to increased interest among researchers globally. To characterize the genetic diversity and intraspecific variation of this parasite in South America, specifically in Colombia, we collected 105 adult parasites from cattle bile ducts in seven Colombian departments (Antioquia, Boyacá, Santander, Cauca, Cundinamarca, Nariño, Norte de Santander, and Santander) to assess the parasite’s phenotypic analyses, genetic diversity, and population structure. A computer image analysis system (CIAS) was applied based on standardized morphological measurements. Liver-fluke size was studied by principal component analysis (PCA). DNA sequences were obtained for nuclear markers such as the 28S, β-tubulin 3, ITS1, ITS2, and the mitochondrial marker Cytochrome Oxidase I (COI). Multiple statistical tests were performed, and the parasite’s population structure was analyzed. Maximum Likelihood (ML) phylogenetic reconstructions were carried out using the sequences obtained herein and sequences available in GenBank. Morphological results revealed that all the obtained individuals matched F. hepatica’s morphology. There was no evidence of high genetic diversity, and the absence of genetic structure at the country-level was notable, possibly caused by a demographic expansion of this trematode in Colombia or the low resolution of the molecular markers employed. Future studies are still needed to unveil the genetic population structure of F. hepatica across the country.

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Diagnóstico post mortem de Fasciola hepatica en bovinos faenados en la planta de beneficio de Sogamoso (Boyacá, Colombia)

Cited by 2 publications

References 4 publications

Seroprevalence and risk factors of bovine fasciolosis in the municipalities of Colombia

Seroprevalence and risk factors of bovine fasciolosis in the municipalities of Colombia

Molecular characterization of Fasciola hepatica in endemic regions of Colombia

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