Diagnostics for Citrus Greening Disease (Huanglongbing): Current and Emerging Technologies

Das, A. K.; Sharma, Susheel Kumar; Thakre, Neha

doi:10.1007/978-981-13-8499-8_26

Cited by 8 publications

(9 citation statements)

References 121 publications

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“…Ca. L. asiaticus ( C Las), is considered the most pathogenic amongst all the Liberibacter species reported until now ( Das et al, 2019 ). This bacterium infects the most active vascular tissue of the tree, phloem, and is harbored by the insect vector Asian citrus psyllid (ACP).…”

Section: Citrus Microbiome and Incurring Huanglongbing: A Case Studymentioning

confidence: 99%

“…This bacterium infects the most active vascular tissue of the tree, phloem, and is harbored by the insect vector Asian citrus psyllid (ACP). HLB is known as the most destructive disease in citrus as of now, incurring enormous citrus production losses the world over ( Bové, 2006 ; Gottwald, 2010 ; Das et al, 2019 ). The mineral deficiency-like symptoms are closely associated and often confuse the researchers; this the disease is correctly diagnosed through PCR-based techniques ( Das, 2004 , 2009 ; Das et al, 2014 ).…”

Section: Citrus Microbiome and Incurring Huanglongbing: A Case Studymentioning

confidence: 99%

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Bioprospecting Microbiome for Soil and Plant Health Management Amidst Huanglongbing Threat in Citrus: A Review

et al. 2022

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Microorganisms have dynamic and complex interactions with their hosts. Diverse microbial communities residing near, on, and within the plants, called phytobiome, are an essential part of plant health and productivity. Exploiting citrus-associated microbiomes represents a scientific approach toward sustained and environment-friendly module of citrus production, though periodically exposed to several threats, with Huanglongbing (HLB) predominantly being most influential. Exploring the composition and function of the citrus microbiome, and possible microbial redesigning under HLB disease pressure has sparked renewed interest in recent times. A concise account of various achievements in understanding the citrus-associated microbiome, in various niche environments viz., rhizosphere, phyllosphere, endosphere, and core microbiota alongside their functional attributes has been thoroughly reviewed and presented. Efforts were also made to analyze the actual role of the citrus microbiome in soil fertility and resilience, interaction with and suppression of invading pathogens along with native microbial communities and their consequences thereupon. Despite the desired potential of the citrus microbiota to counter different pathogenic diseases, utilizing the citrus microbiome for beneficial applications at the field level is yet to be translated as a commercial product. We anticipate that advancement in multiomics technologies, high-throughput sequencing and culturing, genome editing tools, artificial intelligence, and microbial consortia will provide some exciting avenues for citrus microbiome research and microbial manipulation to improve the health and productivity of citrus plants.

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Section: Citrus Microbiome and Incurring Huanglongbing: A Case Studymentioning

confidence: 99%

Section: Citrus Microbiome and Incurring Huanglongbing: A Case Studymentioning

confidence: 99%

Bioprospecting Microbiome for Soil and Plant Health Management Amidst Huanglongbing Threat in Citrus: A Review

et al. 2022

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“…1,2 Citrus greening disease has been associated with infection by at least three species of phloem-restricted, fastidious bacteria belonging to the genus 'Candidatus (Ca) Liberibacter'. 3,4 'Ca. Liberibacter asiaticus' (CLas) is the most widespread and damaging species, having been introduced from Asia into the Americas [5][6][7] and recently, into Africa.…”

Section: Introductionmentioning

confidence: 99%

“…Liberibacter asiaticus' (CLas) is the most widespread and damaging species, having been introduced from Asia into the Americas [5][6][7] and recently, into Africa. 4,[8][9][10] Infection by CLas disrupts phloem function and impairs the systemic transport of carbohydrates and nutrients. 11 Characteristic symptoms of citrus greening disease are foliar chlorosis, twig dieback, misshapen and discolored fruit, and stunting, decline and eventual death of the tree.…”

Section: Introductionmentioning

confidence: 99%

“…11 Characteristic symptoms of citrus greening disease are foliar chlorosis, twig dieback, misshapen and discolored fruit, and stunting, decline and eventual death of the tree. 2,4,12,13 The CLas bacterial pathogen is transmitted in a circulative and propagative manner by the Asian citrus psyllid (ACP) Diaphorina citri (Kuwayama) (Hemiptera: Sternorrhyncha). [14][15][16][17] 17 Management of greening disease has depended on suppression of the ACP population size to reduce the frequency of CLas transmission.…”

Section: Introductionmentioning

confidence: 99%

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UV‐LASER adjuvant–surfactant‐facilitated delivery of mobile dsRNA to tomato plant vasculature and evidence of biological activity by gene knockdown in the potato psyllid

Thakre,

Carver,

Paredes‐Montero

et al. 2024

Pest Management Science

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BackgroundDouble‐stranded RNA (dsRNA) biopesticides are of interest for abatement of insect vectors of pathogenic bacteria such as ‘Candidatus Liberibacter’, which infects both its psyllid and plant hosts. Silencing of genes essential for psyllids, or for Liberibacter invasion, is anticipated to lead to mortality or impeded bacterial multiplication. Foliar delivery is preferred for biopesticide application; however, the cuticle impedes dsRNA penetration into vasculature. Here, conditions were established for wounding tomato leaves using UV‐LASER to promote dsRNA penetration into leaves and vasculature.ResultsUV‐LASER treatment with application of select adjuvants‐surfactants resulted in vascular delivery of 100‐, 300‐, and 600‐base pair (bp) dsRNAs that in general were correlated with size. The 100‐bp dsRNA required no pre‐treatment, while 300‐bp and 600‐bp dsRNAs entered the vasculature after UV‐LASER treatment only, and UV‐LASER‐adjuvant/surfactant treatment, respectively. Of six adjuvant/surfactants evaluated, the plant‐derived oil combined with an anionic organosilicon compound performed most optimally. Localization of dsRNAs in the tomato vasculature was documented by fluorometry and fluorescence confocal microscopy. The biological activity of in planta‐delivered dsRNA (200‐250bp) was determined by feeding third instar psyllids on tomato leaves post‐UV‐LASER‐adjuvant/surfactant treatment, with or without psyllid cdc42‐ and gelsolin dsRNAs. Gene knockdown was quantified by quantitative, real‐time RT‐PCR amplification. At 10‐days post‐IAP, knockdown of cdc42 and gelsolin expression was 61% and 56%, respectively, indicating the dsRNAs delivered to the tomato vasculature were mobile and biologically active.ConclusionResults indicated that UV‐LASER‐adjuvant/surfactant treatments facilitated the delivery of mobile, biologically‐active dsRNA molecules to the plant vasculature.This article is protected by copyright. All rights reserved.

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Genetic diversity and population structure of ‘Candidatus Liberibacter asiaticus’ associated with citrus Huanglongbing in India based on the prophage types

Das

Chichghare

Sharma

et al. 2021

World J Microbiol Biotechnol

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Diagnostics for Citrus Greening Disease (Huanglongbing): Current and Emerging Technologies

Cited by 8 publications

References 121 publications

Bioprospecting Microbiome for Soil and Plant Health Management Amidst Huanglongbing Threat in Citrus: A Review

Bioprospecting Microbiome for Soil and Plant Health Management Amidst Huanglongbing Threat in Citrus: A Review

UV‐LASER adjuvant–surfactant‐facilitated delivery of mobile dsRNA to tomato plant vasculature and evidence of biological activity by gene knockdown in the potato psyllid

Genetic diversity and population structure of ‘Candidatus Liberibacter asiaticus’ associated with citrus Huanglongbing in India based on the prophage types

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