Dialdehyde cellulose-crosslinked collagen and its physicochemical properties

Pietrucha, Krystyna; Safandowska, Marta

doi:10.1016/j.procbio.2015.09.025

Cited by 60 publications

(52 citation statements)

References 34 publications

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“…Earlier studies indicated that the secondary triple helix structure of collagen present within Col scaffolds remained unchanged, thus allowing the optimal biological properties of the scaffold to be used in regenerative medicine. [3][4][5][6] In the present study, the presence or absence of cross-links, CS and HA in the collagen sponges had a significant effect on the overall structure and distribution of the interconnected pores (Figs. 1, 2).…”

Section: Discussionmentioning

confidence: 49%

“…Thus, the implants obtained not only close the wound but also stimulate the self-repair or self-regeneration of the wound. 2 The favorable physical and chemical properties of collagenous (Col) scaffolds for regenerative medicine have been described by Pietrucha et al [3][4][5][6] The Col materials create a supportive microenvironment for fibroblasts, osteoblasts and embryonic nerve cells. 2,[6][7][8] Wound fibroblasts are thought to enter the scaffold from the margins of the wound and participate in scar formation.…”

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confidence: 99%

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Collagenous scaffolds supplemented with hyaluronic acid and chondroitin sulfate used for wound fibroblast and embryonic nerve cell culture

et al. 2017

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Background. Tissue engineering is a strategy aimed at improving the regeneration of injured tissues.Objectives. The aim of the present study was to determine whether a tri-copolymer composed of crosslinked collagen, chondroitin sulfate and hyaluronic acid (Col + CS + HA) provides a better environment for fibroblast and embryonic nerve cell culture than a collagenous scaffold (Col).Material and methods. The porosity of each of the matrices was characterized with a scanning electron microscope. Fibroblasts were isolated from rat wound granulation tissue (polypropylene net implanted subcutaneously). Embryonic nerve cells were obtained from the brains of rat embryos. The cells were applied to scaffolds and then stained with bisbenzimide to calculate cell entrapment within the material. The metabolic activity of the cells cultured within the scaffolds was tested using the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.Results. The Col scaffolds had a homogenously porous structure with a pore diameter of 50 μm for 70% of pores. The pore diameter in the tri-copolymer (Col + HA + CS) ranged from 24 to 160 μm (95% of total pore volume). Four times more cells (fibroblasts and embryonic nerve cells) were trapped within the superficial part of the collagenous scaffold than that of the tri-copolymer. On the third day of culture the metabolic activity of the fibroblasts within the 2 tested scaffolds was significantly higher than in the control conditions (cell culture on a laminin-coated surface). Also, the embryonic nerve cells demonstrated increased metabolic activity in Col + CS + HA scaffolds than the Col scaffolds.Conclusions. Both fibroblasts and embryonic nerve cells could be seeded within the 2 tested scaffolds. Both the scaffolds provide good conditions for fibroblast culture. However, the Col + CS + HA tri-copolymer is preferable for embryonic nerve cell engineering.

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Section: Discussionmentioning

confidence: 49%

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confidence: 99%

Collagenous scaffolds supplemented with hyaluronic acid and chondroitin sulfate used for wound fibroblast and embryonic nerve cell culture

et al. 2017

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“…The amide II and III bands are due to the N-H bending vibrations and C-N stretching vibrations respectively. 26 As shown in Fig. 2b, the peak situates around 1037 cm À1 might be related to glycerol.…”

Section: Formation Of the Interactions Between Gelatin Silica Nanopamentioning

confidence: 84%

Superhydrophobic coatings on gelatin-based films: fabrication, characterization and cytotoxicity studies

Chen

Guo

et al. 2018

RSC Adv.

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“…First, OBC is an attractive crosslinker to stabilize COL owing to its higher stability and biocompatibility than other similar compounds as well as its natural abundance . COL crosslinked with OBC displayed an obvious increase of thermal and enzymatic stability . Second, the OBC‐COL sponge fillers provide an interconnected architecture with porosity and pore size of 71.6% and 58 µm, a good mimicry of nanostructures in ECM .…”

Section: Discussionmentioning

confidence: 99%

Repairing Transected Peripheral Nerve Using a Biomimetic Nerve Guidance Conduit Containing Intraluminal Sponge Fillers

Hou

Wang

Zhang

et al. 2019

Adv Healthcare Materials

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Nerve guide conduits (NGCs) with geometric design have shown significant advantages in guidance of nerve reinnervation across the defect of injured peripheral nerves. It is realized that intraluminal fillers with distinctive structure can effectively provide an inner guidance for sprouting of axons and improve the permeability of NGC. In this work, a poly(lactic‐co‐glycolic acid) (PLGA) NGC is prepared containing intraluminal sponge fillers (labeled as ISF‐NGC) and used for reconstruction of a rat sciatic nerve with a 10 mm gap. For comparison, the same procedure is applied to a single hollow PLGA NGC (labeled as H‐NGC) and an autologous nerve. As evidenced by significantly improved nerve morphology and function, the ISF‐NGC achieves a superior nerve repair effect over H‐NGC, which is comparable to autologous nerve grafting. It is likely that the H‐NGC only provides a protected tunnel for nerve fiber regrowth and axonal extension, while ISF‐NGC offers an extracellular matrix‐mimetic architecture as autograft to provide contact guidance for nerve reinnervation. This newly developed ISF‐NGC is a promising candidate to aid nerve reinnervation across longer gaps commonly encountered in clinical cases.

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Dialdehyde cellulose-crosslinked collagen and its physicochemical properties

Cited by 60 publications

References 34 publications

Collagenous scaffolds supplemented with hyaluronic acid and chondroitin sulfate used for wound fibroblast and embryonic nerve cell culture

Collagenous scaffolds supplemented with hyaluronic acid and chondroitin sulfate used for wound fibroblast and embryonic nerve cell culture

Superhydrophobic coatings on gelatin-based films: fabrication, characterization and cytotoxicity studies

Repairing Transected Peripheral Nerve Using a Biomimetic Nerve Guidance Conduit Containing Intraluminal Sponge Fillers

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