Diallel Analysis of Genetic Resistance to<i>Cronartium quercuum</i>f. sp.<i>fusiforme</i>in Slash Pine

Griggs, Margene M.

doi:10.1094/phyto-72-816

Cited by 13 publications

(4 citation statements)

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“…Miyabe ex Shirai f.sp. fusiforme ( Cqf ) genetic interactions with Pinus hosts [ 44 , 45 ] led to mapping of F usiform r ust resistance 1 ( Fr1 ; [ 46 ]) to LG2 [ 47 , 48 ]. P. lambertiana Cr1 for white pine blister rust resistance [ 49 ] was also mapped to the same linkage group using syntenic markers [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

Decoding the massive genome of loblolly pine using haploid DNA and novel assembly strategies

et al. 2014

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BackgroundThe size and complexity of conifer genomes has, until now, prevented full genome sequencing and assembly. The large research community and economic importance of loblolly pine, Pinus taeda L., made it an early candidate for reference sequence determination.ResultsWe develop a novel strategy to sequence the genome of loblolly pine that combines unique aspects of pine reproductive biology and genome assembly methodology. We use a whole genome shotgun approach relying primarily on next generation sequence generated from a single haploid seed megagametophyte from a loblolly pine tree, 20-1010, that has been used in industrial forest tree breeding. The resulting sequence and assembly was used to generate a draft genome spanning 23.2 Gbp and containing 20.1 Gbp with an N50 scaffold size of 66.9 kbp, making it a significant improvement over available conifer genomes. The long scaffold lengths allow the annotation of 50,172 gene models with intron lengths averaging over 2.7 kbp and sometimes exceeding 100 kbp in length. Analysis of orthologous gene sets identifies gene families that may be unique to conifers. We further characterize and expand the existing repeat library based on the de novo analysis of the repetitive content, estimated to encompass 82% of the genome.ConclusionsIn addition to its value as a resource for researchers and breeders, the loblolly pine genome sequence and assembly reported here demonstrates a novel approach to sequencing the large and complex genomes of this important group of plants that can now be widely applied.

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Section: Resultsmentioning

confidence: 99%

Decoding the massive genome of loblolly pine using haploid DNA and novel assembly strategies

et al. 2014

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“…Major importance of additive genetic effects for twist rust susceptibility was confirmed by large differences in GCA estimates of the two sets of parents and nonsignificant SCA effects in controlled crosses. In earlier studies of the inheritance of fusiform rust resistance in slash and loblolly pines, Sluder (1988) and Griggs & Walkinshaw (1982) showed highly significant variation in GCA for this trait and no significant variation in SCA. Because GCA effects were predominant, selection for reduced rust susceptibility based on WP or polycross progeny tests could be easily included in the management of the two existing breeding populations.…”

Section: Discussionmentioning

confidence: 87%

Genetic variation within and between populations of Pinus sylvestris L. (Scots pine) for susceptibility to Melampsora pinitorqua Rostr. (pine twist rust)

Quencez¹,

Bastien²

2008

Heredity

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The genetic variability of Scots pine (Pinus sylvestris L.) for twist rust susceptibility and the consequences for genetic improvement of the species were evaluated after artificial inoculation of 1‐year‐old seedlings in greenhouse tests. Wind‐pollinated progenies, factorial and incomplete diallel mating designs were used to compare two natural populations of Scots pine (Haguenau, France, and Taborz, Poland) and their hybrids (Haguenau × Taborz). Families from Taborz were significantly (P < 0.001) less susceptible to twist rust than those from Haguenau. Inter‐population hybrids were as susceptible as the Haguenau population. No clear relationship between severity of infection, shoot length and phenological stage at time of inoculation was observed in intra‐ and interpopulation crosses. Differences observed between the two natural populations for their response to twist rust are suggested to be the consequence of local adaptation simultaneously for climatic conditions and pathogen pressure. Within each of the two populations, the effects of general combining abilities (GCA) for rust susceptibility were predominant, although estimation of individual heritabilities remained at a moderate level (0.30–0.54). In interpopulation crosses, GCA effects of Haguenau and Taborz populations were still predominant. Moreover, hybrid performance could be predicted with confidence based on the intrapopulation GCA values of parents. Genetic gain on twist rust resistance could be achieved quickly through intrapopulation mass or backward selection based on wind‐pollinated progeny tests.

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“…The OP families were useful for recognizing resistant and susceptible selections, and the full sib data led Jewell [12] to propose that resistance in slash pine was controlled by a dominant single gene, but later Jewell and Mallett [13], with additional disease data from full-sib seedlings from resistant selections 8-7 and 18-27, deemed resistance in slash pine to be more complex. Kinloch and Walkinshaw [14], in a reanalysis of an earlier study by Griggs and Walkinshaw [15] that used full-sib slash pine families from a five parent diallel cross (including resistant parents, 8-7 and 18-27 used in works [12,13]) challenged with basidiospore inocula derived from two single galls, reported fusiform rust resistance involving dominant single host genes, two in that study, and based on the data hypothesized gene-for-gene interactions. Powers [16] and Kuhlman and Matthews [17] reported methods for the development of single-genotype Cqf isolates with the Kuhlman and Matthews work showing virulence variation among single-genotype isolates derived from the same single gall.…”

Section: Introductionmentioning

confidence: 99%

Identification of Nine Pathotype-Specific Genes Conferring Resistance to Fusiform Rust in Loblolly Pine (Pinus taeda L.)

Amerson

Nelson

Kubisiak

et al. 2015

Forests

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Nearly two decades of research on the host-pathogen interaction in fusiform rust of loblolly pine is detailed. Results clearly indicate that pathotype-specific genes in the host interacting with pathogen avirulence cause resistance as defined by the non-gall phenotype under favorable environmental conditions for disease development. In particular, nine fusiform rust resistance genes (Fr genes) are described here including the specific methods to determine each and their localization on the reference genetic map of loblolly pine. Understanding how these and other apparent Fr genes in loblolly pine and other rust-susceptible pines impact resistance screening, parental and progeny selection, and family and clonal deployment is an important area in forest genetics research and OPEN ACCESSForests 2015, 6 2740 operational tree breeding. The documentation of these Fr genes is a key piece of information towards gaining that understanding and ultimately improving breeding and deployment strategies.

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Diallel Analysis of Genetic Resistance toCronartium quercuumf. sp.fusiformein Slash Pine

Cited by 13 publications

References 0 publications

Decoding the massive genome of loblolly pine using haploid DNA and novel assembly strategies

Decoding the massive genome of loblolly pine using haploid DNA and novel assembly strategies

Genetic variation within and between populations of Pinus sylvestris L. (Scots pine) for susceptibility to Melampsora pinitorqua Rostr. (pine twist rust)

Identification of Nine Pathotype-Specific Genes Conferring Resistance to Fusiform Rust in Loblolly Pine (Pinus taeda L.)

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