Diallel Crossing Among Doubled Haploids of Cucumber Reveals Significant Reciprocal-cross Differences

Shen, Jia; Dirks, Robert; Havey, Michael J.

doi:10.21273/jashs.140.2.178

Cited by 14 publications

(18 citation statements)

References 29 publications

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“…Mitochondrial-nuclear interactions are important for plant performance ( Kihira 1982 ; Shen et al 2015 ), and mitochondrial function can be affected by stress ( Millar et al 2003 ; Vanlerberghe et al 2009 ; Gill and Tuteja 2010 ; Juszczuk et al 2011 ; Atkin and Macherel 2009 ; Mróz et al 2015 ), mutations or rearrangements in the mtDNA ( Lauer et al 1990 ; Newton et al 1990 ; Hunt and Newton 1991 ; Gu et al 1993 ; Hartmann et al 1994 ; Marienfeld and Newton 1994 ; Sakamoto et al 1996 ; Janska et al 1998 ; Lilly et al 2001 ; Kuzmin et al 2005 ; Juszczuk et al 2007 ), or substochiometric shifting ( Shedge et al 2007 ). Mitochondrial mutations are often deleterious and show chlorosis of leaves, slower growth, and reduced fertility as exemplified by the NCS mutations of maize ( Coe 1983 ; Newton and Coe 1986 ; Newton 1995 ), chm -conditioned mutants of Arabidopsis thaliana ( Martínez-Zapater et al 1992 ; Abdelnoor et al 2003 ), and the mosaic (MSC) mutants of cucumber ( Malepszy et al 1996 ; Lilly et al 2001 ; Bartoszewski et al 2007 ).…”

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confidence: 99%

Transcriptome Analyses of Mosaic (MSC) Mitochondrial Mutants of Cucumber in a Highly Inbred Nuclear Background

Mróz

Akker

Bernat

et al. 2018

G3 Genes|Genomes|Genetics

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Cucumber (Cucumis sativus L.) has a large, paternally transmitted mitochondrial genome. Cucumber plants regenerated from cell cultures occasionally show paternally transmitted mosaic (MSC) phenotypes, characterized by slower growth, chlorotic patterns on the leaves and fruit, lower fertility, and rearrangements in their mitochondrial DNAs (mtDNAs). MSC lines 3, 12, and 16 originated from different cell cultures all established using the highly inbred, wild-type line B. These MSC lines possess different rearrangements and under-represented regions in their mtDNAs. We completed RNA-seq on normalized and non-normalized cDNA libraries from MSC3, MSC12, and MSC16 to study their nuclear gene-expression profiles relative to inbred B. Results from both libraries indicated that gene expression in MSC12 and MSC16 were more similar to each other than MSC3. Forty-one differentially expressed genes (DEGs) were upregulated and one downregulated in the MSC lines relative to B. Gene functional classifications revealed that more than half of these DEGs are associated with stress-response pathways. Consistent with this observation, we detected elevated levels of hydrogen peroxide throughout leaf tissue in all MSC lines compared to wild-type line B. These results demonstrate that independently produced MSC lines with different mitochondrial polymorphisms show unique and shared nuclear responses. This study revealed genes associated with stress response that could become selection targets to develop cucumber cultivars with increased stress tolerance, and further support of cucumber as a model plant to study nuclear-mitochondrial interactions.

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confidence: 99%

Transcriptome Analyses of Mosaic (MSC) Mitochondrial Mutants of Cucumber in a Highly Inbred Nuclear Background

Mróz

Akker

Bernat

et al. 2018

G3 Genes|Genomes|Genetics

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“…Seeds from these crosses were grown in sterilized vermiculite in wooden ats at 30° C and progenies scored for frequencies of wild-type versus MSC progenies. Selfed progenies from one F 2 plant (C10115HQ) with the genotype Psm -/-showed higher numbers of wild-type progenies in crosses with MSC3 as the male, and was crossed as the male to a doubled haploid plant from inbred 9930 (Shen et al 2015). A single hybrid plant was self-pollinated to produce segregating F 2 progenies which were crossed with MSC3 as the male.…”

Section: Methodsmentioning

confidence: 99%

Genetic Mapping of Mitochondrial Sorting of the MSC3 Mosaic Mutant of Cucumber

Lt¹,

Havey²

2021

Preprint

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Passage of the highly inbred line ‘B’ of cucumber through cell cultures has produced regenerated plants with a mosaic (MSC) phenotype on cotyledons and leaves, as well as rearrangements in the mitochondrial DNA. Both of these characteristics show paternal transmission. MSC3 and MSC16 were derived from independent cell-culture experiments and have distinct mosaic phenotypes and different under-represented regions in their mitochondrial DNAs. A nuclear locus, Psm for paternal transmission of mitochondria, conditions a high proportion of wild type progenies when MSC16 is crossed as the male with female plants carrying the Psm- allele. Plants with homozygous genotypes at Psm were crossed with both MSC3 and MSC16, and segregation of wild-type versus mosaic progenies in these families were not consistent suggesting that sorting of wild-type progenies from crosses with MSC3 and MSC16 have different genetic bases. We identified cucumber plants that produced a high proportion of wild-type progenies in crosses with MSC3 as the male parent. Plants from a segregating F2 family were crossed with MSC3 as the male and progenies scored for numbers of mosaic versus wild-type progenies. The same F2 plants were genotyped-by-sequencing and single nucleotide polymorphisms identified for genetic mapping. Quantitative analysis of the proportion of wild-type testcross progenies identified a major quantitative trait locus (QTL) in the same genomic region as the Psm locus; however the most significant SNP associated with this QTL was located approximately 856 kilobases from Psm. Eventual identification of a candidate gene controlling this unique mitochondrial sorting in cucumber should reveal important aspects of mitochondrial-nuclear interactions affecting the prevalence of specific mitochondrial DNAs.

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“…The set of simple sequence repeat (SSR) markers for genotyping was derived from previously developed markers described by Ren et al ( 2009 ), Yang et al ( 2012 ), and Shen et al ( 2015 ). All the markers were tested in silico for polymorphism by comparing of corresponding microsatellite regions in Gy14 and B10 genomic sequences.…”

Section: Methodsmentioning

confidence: 99%

“…All the markers were tested in silico for polymorphism by comparing of corresponding microsatellite regions in Gy14 and B10 genomic sequences. The set of 218 SSR markers, i.e., 86 SSRs of Ren et al ( 2009 ), 113 of Yang et al ( 2012 ), and 18 of Shen et al ( 2015 ), were subsequently analyzed on parental lines and RILs (Słomnicka et al 2015 ). Finally, the set of 52 high quality SSR markers was used for genotyping (Supplementary File 2 ).…”

Section: Methodsmentioning

confidence: 99%

Genetic mapping of psl locus and quantitative trait loci for angular leaf spot resistance in cucumber (Cucumis sativus L.)

et al. 2018

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One of the most important cucumber diseases is bacterial angular leaf spot (ALS), whose increased occurrence in open-field production has been observed over the last years. To map ALS resistance genes, a recombinant inbred line (RIL) mapping population was developed from a narrow cross of cucumber line Gy14 carrying psl resistance gene and susceptible B10 line. Parental lines and RILs were tested under growth chamber conditions as well as in the field for angular leaf spot symptoms. Based on simple sequence repeat and DArTseq, genotyping a genetic map was constructed, which contained 717 loci in seven linkage groups, spanning 599.7 cM with 0.84 cM on average between markers. Monogenic inheritance of the lack of chlorotic halo around the lesions, which is typical for ALS resistance and related with the presence of recessive psl resistance gene, was confirmed. The psl locus was mapped on cucumber chromosome 5. Two major quantitative trait loci (QTL) psl5.1 and psl5.2 related to disease severity were found and located next to each other on chromosome 5; moreover, psl5.1 was co-located with psl locus. Identified QTL were validated in the field experiment. Constructed genetic map and markers linked to ALS resistance loci are novel resources that can contribute to cucumber breeding programs.Electronic supplementary materialThe online version of this article (10.1007/s11032-018-0866-2) contains supplementary material, which is available to authorized users.

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Diallel Crossing Among Doubled Haploids of Cucumber Reveals Significant Reciprocal-cross Differences

Cited by 14 publications

References 29 publications

Transcriptome Analyses of Mosaic (MSC) Mitochondrial Mutants of Cucumber in a Highly Inbred Nuclear Background

Transcriptome Analyses of Mosaic (MSC) Mitochondrial Mutants of Cucumber in a Highly Inbred Nuclear Background

Genetic Mapping of Mitochondrial Sorting of the MSC3 Mosaic Mutant of Cucumber

Genetic mapping of psl locus and quantitative trait loci for angular leaf spot resistance in cucumber (Cucumis sativus L.)

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