Diallyl disulfide induces apoptosis in human colon cancer cell line (COLO 205) through the induction of reactive oxygen species, endoplasmic reticulum stress, caspases casade and mitochondrial-dependent pathways

Yang, Jai Sing; Chen, Guang Wei; Hsia, Te Chun; Ho, Heng Chien; Ho, Chao-Ching; Meng, Lin; Lin, Song; Yeh, Ru Duan; Ip, Siu Wan; Lu, Hsu Fung; Chung, Jing Gung

doi:10.1016/j.fct.2008.10.032

Cited by 111 publications

(128 citation statements)

References 28 publications

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“…The antiproliferative property of DADS in cultured human colon tumor cells (HCT-116 and COLO 205) and the prostate cancer cell line (PC-3) is related to its ability to decrease the proportion of cells in the G 1 phase and to increase the proportion of cells in the G 2 /M phase (12,15,16). Changes in activity may also result from a combination of the quantity and activity of specific cellular proteins.…”

Section: Introductionmentioning

confidence: 99%

Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide

Ling

Wen

et al. 2010

Braz J Med Biol Res

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Section: Introductionmentioning

confidence: 99%

Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide

Ling

Wen

et al. 2010

Braz J Med Biol Res

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“…5 cells/well of GBM 8401 cells in a 12-well plate were treated with 0, 10, and 20 μM of AITC and then were incubated for 24 and 48 h. Cells were directly examined and were photographed under contrast-phase microscope (24).…”

Section: Cell Morphological Examination About 2x10mentioning

confidence: 99%

“…Cells were incubated and grown for 24 h. Cells were stained by 4'-6-diamidino-2-phenylindole (DAPI, Molecular Probes/Invitrogen Corp., Eugene, OR, USA) and were examined and photographed under fluorescence microscopy (24).…”

Section: Assessment Of Apoptosis Morphology By Dapi Stainingmentioning

confidence: 99%

Allyl isothiocyanate triggers G2/M phase arrest and apoptosis in human brain malignant glioma GBM 8401 cells through a mitochondria-dependent pathway

Chen

Chen²,

Lu³

et al. 2010

Oncol Rep

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Abstract. Isothiocyanates (ITCs) are present as glucosinolates in various cruciferous vegetables. Allyl isothiocyanate (AITC) is one of the common naturally occurring isothiocyanates. Recent studies have shown that AITC significantly inhibited survival of leukemia HL-60, bladder cancer UM-UC-3 and colon cancer HT-29 cells in vitro. In this study, we demonstrate that AITC significantly decreased proliferation and viability of human brain malignant glioma GBM 8401 cells in a dose-dependent manner with IC 50 9.25±0.69 μM for 24 h-treatment. The analysis of cell cycle distribution also showed that AITC induced significantly G2/M arrest and sub-G1 phase (apoptotic population) in GBM 8401 cells. AITC markedly reduced the CDK1/cyclin B activity and protein levels by CDK1 activity assay and Western blot analysis. AITC-induced apoptotic cell death and this evidence was confirmed by morphological assessment and DAPI staining. Pretreatment with specific inhibitors of caspase-3 (Z-DEVE-FMK) and -9 (Z-LEHD-FMK) significantly reduced caspase-3 and -9 activity in GBM 8401 cells. Western blot analysis and colorimetric assays also displayed that AITC caused a time-dependent increase in cytosolic cytochrome c, pro-caspase-9, Apaf-1, AIF, Endo G and the stimulated caspase-9 and -3 activity. Our results suggest that AITC is a potent anti-human brain malignant glioma drug and it shows a remarkable action on cell cycle arrest before commitment for apoptosis is reached.

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“…After HCT treatment, cells were fixed in 4% paraformaldehyde for 30 min and incubated with 1 μg/ml of DAPI staining solution for 30 min in the dark. The apoptotic cells were observed through fluorescence microscopy (Zeiss, Oberköchen, Germany) (25).…”

Section: Preparation Of Hct Hottuynia Cordatamentioning

confidence: 99%

Houttuynia cordata Thunb extract induces apoptosis through mitochondrial-dependent pathway in HT-29 human colon adenocarcinoma cells

Tang

Yang²,

Lin³

et al. 2009

Oncol Rep

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Abstract. The Houttuynia cordata Thunb (HCT) extract has been used as a traditional Chinese herb medicine and as well as an effective drug for treating allergic inflammation for thousands of years. In this study, we investigated the anticancer activity of HCT and its molecular mechanisms in the human colon adenocarcinoma cell line HT-29. HCT inhibited HT-29 cell viability in a dose-and time-dependent manner by MTT assay. Treatment with 450 μg/ml of HCT for 48 and 72 h led to DNA damage and apoptosis by DAPI staining and comet assay. HCT increased reactive oxygen species production and decreased the levels of mitochondria membrane potential (MMP) in HT-29 cells by flow cytometry analysis. HCT caused the release of cytochrome c, Apaf-1, pro-caspase-9 and AIF from mitochondria via a decrease of the MMP. The decrease of MMP was then associated with a decrease in the ratio of Bax/Bcl-2 and activation of caspase-9 and -3 by Western blotting and caspase activity assay. Caspase-9 and -3 inhibitors almost completely suppressed HCT-induced caspase-9 and -3 activities. Our results demonstrated that the HCT-induced apoptosis in human colon adeno-carcinoma cell line HT-29 might be related to a mitochondrial-dependent pathway.

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Diallyl disulfide induces apoptosis in human colon cancer cell line (COLO 205) through the induction of reactive oxygen species, endoplasmic reticulum stress, caspases casade and mitochondrial-dependent pathways

Cited by 111 publications

References 28 publications

Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide

Growth inhibitory effect and Chk1-dependent signaling involved in G2/M arrest on human gastric cancer cells induced by diallyl disulfide

Allyl isothiocyanate triggers G2/M phase arrest and apoptosis in human brain malignant glioma GBM 8401 cells through a mitochondria-dependent pathway

Houttuynia cordata Thunb extract induces apoptosis through mitochondrial-dependent pathway in HT-29 human colon adenocarcinoma cells

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