Diallyl disulfide inhibits the proliferation of human tumor cells in culture

Abstract

Diallyl disulfide (DADS), an oil-soluble organosulfur compound in processed garlic, was more effective in inhibiting the in vitro growth of human tumor cell lines: HCT-15 (colon), A549 (lung), and SK MEL-2 (skin) than isomolar quantities of the water-soluble compound S-allyl cysteine (SAC). Addition of DADS (100 microM) was cytostatic to all three cell lines. The importance of the allyl and the disulfide groups were revealed by the lack of a comparable depression in the growth of HCT-15 cells exposed to its saturated analogue, dipropyl disulfide (DPDS). Treatment with DADS also resulted in a dose-dependent increase in intracellular free calcium in cells. A dose-dependent decrease in the activity of calcium-dependent ATPase enzyme occurred in HCT-15 cells exposed to increasing quantities of DADS. A correlation (r = -0.975) was found between the intracellular free calcium levels and the Ca-ATPase activity in DADS-treated cells. These studies document that DADS, a constituent of garlic oil, is an effective inhibitor of the growth of human neoplastic cells. Alterations in calcium hemostasis are likely involved in the growth inhibition/cytotoxicity caused by DADS.

Growth Suppressing Effect of Garlic Compound Diallyl Disulfide on Prostate Cancer Cell Line (PC-3) in Vitro

“…Further, the antiproliferative activity of diallyl disulfide against colon (HCT-15), lung (A549), skin (SK MEL-2) and breast (MCF-7) cancer cells were also reported. 22,23) In the present study, we confirmed the antiproliferative effect of diallyl disulfide on prostate cancer cell line PC-3 in vitro. In this in- Pinto et al 24) described the antiproliferative effect of other minor organosulfur compounds of garlic such as S-allylcysteine, S-allylmercaptocysteine and diallyl sulfide in LNCaP cells.…”

Traditional Chinese medicine in the prevention and treatment of cancer and cancer metastasis

“…The antiproliferative effects of the extracts are associated with the induction of apoptosis (20,57). Morphological changes and DNA fragmentation have been observed in cells treated with DADs and DATs (58,59). Furthermore, a previous study reported that DAS induced the apoptosis of solid tumour cells, according to the results of sub-G 1 fraction and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (60).…”

Efficacy of turmeric (Curcuma longa) and garlic (Allium sativum) on Eimeria species in broilers

“…There are numerous reports indicating the efficacy of garlic in the prevention and treatment of a variety of diseases and for validating its traditional uses. For instance, garlic has been described to exhibit broad antimicrobial activity against bacteria ,fungi and virus [25], [26], [27], antitumor activity [28], as well as antithrombotic, antiarthritic, hypolipidemic, and hypoglycemic activities [30,31] Moreover, garlic has been reported to be effective against diverse parasites such as Amoeba [32], Leishmania [33], Trypanosoma [34] and Cryptosporidium [35]. The efficacy of garlic on Coccidia infections has been reported in rabbits [36].…”