Diamide induces reversible changes in morphology, cytoskeleton and cell-cell coupling in lens epithelial cells

Hyperbaric Oxygen in vivo Accelerates the Loss of Cytoskeletal Proteins and MIP26 in Guinea Pig Lens Nucleus

“…The formation of disulfide-crosslinked high molecular weight aggregates, as observed in this study, is a common feature of oxidatively damaged cytoskeletal proteins Graceffa, Adam and Lehman, 1993 ;Schwartz et al, 1987 ;Zaremba et al, 1984). Lens epithelial cells which have been exposed to sulfhydryl oxidants such as H # O # or diamide suffer a severe disruption of cytoskeletal protein organization (Ikebe et al, 1989 ;Prescott et al, 1991), and cytoskeletal components have been reported to be present in the high molecular weight aggregated protein of human senile cataracts (Roy et al, 1984). Two recent studies using oxidative models for cataract in vivo (buthionine sulfoxamine cataract in the mouse and selenite cataract in the rat) have also demonstrated a heightened loss of lens cytoskeletal proteins in the early stages of cataract (Calvin et al, 1992 ;Matsushima et al, 1997).…”

Lipids and the ocular lens

“…Fatty acid exposure results in bleb formation where fatty acid molecules accumulate in the cells ( 174 ). Bleb formation, disruption of the actin cortical network, and the redistribution of actin are related ( 177,178 ) and contribute to lens opacity. oxygen may serve to keep oxygen in the outer regions of the lens long enough for the mitochondria to degrade it.…”