Diamide Insecticides: Efficacy, Toxicity and Analytical Methods for Residue Monitoring in Food Samples

Abstract

Anthranilic diamide is a new commercial group of insecticides that target insect ryanodine receptors, causing uncontrolled calcium ion release and depletion, which prevents muscular contraction. Due to the high advantages of diamide over other insecticide groups, it has been applied to control a variety of insect pests on a wide range of agricultural crops. The development of this group has encouraged agricultural producers to increase their reliance on it to protect crops from pest damage, resulting in increase residues in agricultural products. To ensure food safety, it is necessary to carefully inspect for diamide residues in food commodities. QuEChERS method for extraction and clean up of diamide insecticide residues was optimized to be used with a variety of analytical techniques, including HPLC-MS, GC-MS, and LC-MS. Recoveries in food samples ranged from 73 to 112% with limits of quantification 0.5 -2 µg kg -1 and a half-life around 1.5 days. More advanced and sensitive methods were developed and optimized for detecting the amounts of diamide residues in food samples and in the related environmental matrices, to manage their impact on human health and environmental safety. Immunoassays, which depend on specific action and interaction between antigen and antibody, have been developed as a rapid and cost-effective technique for specific monitoring insecticide residues in various food and environmental samples. Advances in immunoassay convert enzyme-linked immunosorbent assay (ELISA) into a very useful tool in residue detection of diamide insecticides in food as addressed in this review article.

Pesticide Residues in Vegetables and Fruits from Farmer Markets and Associated Dietary Risks

“…Chlorantraniliprole is one of the insecticides detected one time only in apple. This insecticide is one of the diamide insecticides that are widely used against a variety of insect pests due to their selectivity and low mammalian toxicity [39][40][41]. Tian et al [42] determined diamide insecticides in mushrooms and found that these insecticides can be effectively analyzed using HPLC-MS/MS with LOD and LOQ of 0.05 and 5 ug kg −1 , respectively, and recovery rates ranging from 73.5-110.2%.…”

Residue Analysis and Risk Exposure Assessment of Multiple Pesticides in Tomato and Strawberry and Their Products from Markets

“…It has been shown that only 0.1% of applied chemical pesticides are effectively used for preventing, controlling, and mitigating pests, while the rest remains in the soil [14] causing toxicity to the biota and thereby entering into the food chain. Therefore, the contamination of fruits, vegetables, and other field crops possess a serious health risk to the public [15][16][17]. The impact of pesticide contamination on food has been well studied in North America, Japan, and many parts of Europe.…”