Diaminobenzidine Photooxidation to Visualize Fluorescent Nanoparticles in Adhering Cultured Cells at Transmission Electron Microscopy

Costanzo, Manuela; Malatesta, Manuela

doi:10.1007/978-1-0716-2675-7_27

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“…Depending on the microscopy technique used (bright-field or phase contrast microscopy, fluorescence microscopy or transmission electron microscopy), NPs can be made detectable by either linking/loading appropriate dyes during the synthetic process or by labelling with specific histochemical staining after their administration. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] Moreover, some nanoconstructs such as quantum dots or nanodiamonds, are characterized by an intrinsic photoluminescence that can be easily detected at fluorescence microscopy in the absence of an additional staining. 17,18 This paper aims at providing an overview of the various microscopical approaches used to investigate in vitro the translocation of NPs through biological barriers, thus highlighting the great contribution of microscopy techniques to the knowledge of the interactions between the living environment and the nanoconstructs.…”

Section: Introductionmentioning

confidence: 99%

Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine

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Malatesta

2022

Eur J Histochem

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Nanoconstructs intended to be used as biomedical tool must be assessed for their capability to cross biological barriers. However, studying in vivo the permeability of biological barriers to nanoparticles is quite difficult due to the many structural and functional factors involved. Therefore, the in vitro modeling of biological barriers -2D cell monocultures, 2D/3D cell co-cultures, microfluidic devices- is gaining more and more relevance in nanomedical research. Microscopy techniques play a crucial role in these studies, as they allow both visualizing nanoparticles inside the biological barrier and evaluating their impact on the barrier components. This paper provides an overview of the various microscopical approaches used to investigate nanoparticle translocation through in vitro biological barrier models. The high number of scientific articles reported highlights the great contribution of the morphological and histochemical approach to the knowledge of the dynamic interactions between nanoconstructs and the living environment.

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