Diamond-based quantum sensing of free radicals in migrating human breast cancer cells

Reyes-San-Martin, Claudia; Elías-Llumbet, Arturo; Escobar-Chaves, Elkin; Manterola, Marcia; Mzyk, Aldona; Schirhagl, Romana

doi:10.1016/j.carbon.2024.119405

Carbon

2024

DOI: 10.1016/j.carbon.2024.119405

|View full text |Cite

Diamond-based quantum sensing of free radicals in migrating human breast cancer cells

Claudia Reyes-San-Martin,

Arturo Elías-Llumbet,

Elkin Escobar-Chaves

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Preprint1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Tumor Spheroid Uptake of Fluorescent Nanodiamonds is limited by Mass Density: a 4D Light-Sheet Assay

Niora,

Dufva,

Jauffred

et al. 2024

Preprint

View full text Add to dashboard Cite

Fluorescent nanodiamonds (FNDs) with nitrogen-vacancy centers are promising candidates for long-term biolabeling and biosensing applications due to their biocompatibility and unique optomagnetic properties. The employment of nanomaterials in cancer therapy and diagnostics, requires a deep understanding of how nanoparticles (NPs) interact with the three-dimensional (3D) tumor environment. We developed a novel approach, the “Tumor-in-a-Tube” platform, using 4D light-sheet microscopy to explore the spatio-temporal dynamics of FNDs with 3D tumor spheroids. By monitoring the real-time NP sedimentation, spheroid penetration, and cellular uptake of FNDs and polystyrene nanoparticles (PNPs), we marked the impact of NP mass density on their spheroid interaction. Unlike PNPs, higher-density FNDs underwent rapid sedimentation, which minimized their effective concentration and hindered the FND – spheroid interactions. This results in constrained intratumoral accumulation, and size-independent uptake and penetration. Longer FND effective-exposure-time promotes size-dependent cell uptake, verified by FND treatment on 2D monolayers. Nonetheless, FNDs exhibited good biocompatibility and long-term spheroid labeling, allowing for cell isolation from different spheroid layers. Our results suggest the need for NP effective-exposure-time calibration in comparative NP assays, in 3D static models. Overall, our platform provides a valuable tool for bridging the gap between 2D and 3D static models in NP assessment, drug delivery, toxicology profiling and translational research.

show abstract

Tumor Spheroid Uptake of Fluorescent Nanodiamonds is limited by Mass Density: a 4D Light-Sheet Assay

Niora,

Dufva,

Jauffred

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Diamond-based quantum sensing of free radicals in migrating human breast cancer cells

Cited by 1 publication

References 74 publications

Tumor Spheroid Uptake of Fluorescent Nanodiamonds is limited by Mass Density: a 4D Light-Sheet Assay

Tumor Spheroid Uptake of Fluorescent Nanodiamonds is limited by Mass Density: a 4D Light-Sheet Assay

Contact Info

Product

Resources

About