Diaphorina citri flavi-like virus localization, transmission, and association with Candidatus Liberibacter asiaticus in its psyllid host

Rashidi, Mahnaz; Lin, Chun-Yi; Britt, Kellee; Batuman, Özgur; Rwahnih, Maher Al; Achor, Diann; Levy, Amit

doi:10.1016/j.virol.2021.12.009

Cited by 6 publications

(4 citation statements)

References 50 publications

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“…The accumulation of the PyCaV virus in the piercingsucking mouthparts and chest viscera inferred that the feeding system of the insect might play a critical role in the keeping and transmission of the virus. The salivary gland is one of the main tissues in psyllid (Diaphorina citri) that carries the DcFLV (Diaphorina citri flavi-like virus) [57]. Through the piercing-sucking mouthpart, the virions of PLRV (Potato leafroll virus) enter green peach aphid (Myzus persicae) and reach the salivary glands through the circulation in the hemolymph, and the virions are released into the saliva to contaminate other fruit plants [58].…”

Section: Discussionmentioning

confidence: 99%

Screen and Character a New Iflavirus Virus in the Fruit Tree Pest Pyrops candelaria

Lin,

Song,

et al. 2024

Preprint

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Pyrops candelaria is one of the common pests of fruit trees, but the research on the pathogenic microorganisms it may carry is very limited. Therefore, it is essential to reveal the pathogenic microbes it carries and their potential hazards. This study found a new virus from the transcriptome of P. candelaria, which was first reported in P. candelaria and named as PyCaV (Pyrops candelaria associated virus). RACE and bioinformatics assay revealed that the full-length of PyCaV is 10855 bp with the polyA tail, containing a single open reading frame (ORF) encoding a polyprotein consisting of 3171 amino acid (aa). The virus has a typical Iflavirus structure, including two rhv domains, a RNA helicase domain (HEL), a 3C cysteine protease domain (Pro), and a RNA-dependent RNA polymerase domain (RdRp). Further phylogenetic analysis revealed that this virus belongs to family Iflaviridae and sequence alignements analysis suggedted PyCaV is new member in unassigned genus of family Iflaviridae. Further in-depth analysis of virus-infection-part showed that PyCaV is distributing in the whole longan lanternfly, including its head, chest, and abdomen, but more PyCaV was identified in the chest. The distribution of PyCaV in different parts of P. candelaria was further explored, which showed that more PyCaV were detected in its piercing-sucking mouthparts and chest viscera. Statistical analysis showed that the PyCaV infection was affected by time and locations.

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Section: Discussionmentioning

confidence: 99%

Screen and Character a New Iflavirus Virus in the Fruit Tree Pest Pyrops candelaria

Lin,

Song,

et al. 2024

Preprint

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“…Our previous studies showed that DcFLV is systemically distributed throughout D. citri and can be vertically transmitted to offspring. The association between DcFLV and C Las was determined by showing that their incidences are positively correlated and both pathogens co-localize in the midgut [ 45 ]. These results suggest that these novel D. citri -associated viruses may not only infect the insect host, but also interact with C Las in the midgut.…”

Section: Discussionmentioning

confidence: 99%

“…Psyllids collected from each region were separated into BugDorm mesh cages that contained pathogen-free citrus plants ( Citrus macrophylla ), at 25 ± 2 °C under a 14 light:10 h dark photoperiod and 60–70% relative humidity in the greenhouse. To obtain whole guts, psyllid adults were put in Eppendorf tubes and sedated on ice for 15 min; each adult was then dissected with sharp forceps in phosphate-buffered saline (1× PBS) solution under a stereomicroscope to isolate the midgut [ 45 ]. At least 50 guts were prepared from each region and concentrated in a 2.0 mL Eppendorf tube with 1× PBS solution by centrifugation at 12,000 rpm at room temperature; the solution was then removed, and the guts were stored at −20 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Considering that the insect midgut plays a critical role for C Las in establishing and colonizing their insect vectors, the involvement of insect-specific virus may affect those pathogens. Rashidi et al [ 45 ] confirmed that both DcACV and DcFLV co-occurred with C Las and showed a positive correlation in the psyllid population in Florida groves, and that DcFLV co-localized with C Las in the midgut and salivary gland of psyllid. The co-existence of both C Las and each D. citri -associated virus needs to be confirmed, especially in the midgut tissue, which serves as a reservoir and provides additional biological or molecular functions interacting with both pathogens.…”

Section: Introductionmentioning

confidence: 99%

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Identifying the Gut Virome of Diaphorina citri from Florida Groves

Lin

Batuman

Levy

2023

Insects

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Asian citrus psyllid (Diaphorina citri) transmits the bacterial pathogen Candidatus Liberibacter asiaticus (CLas), the putative causative agent of citrus Huanglongbing disease (HLB). Insect-specific viruses can act against insects as their natural enemies, and recently, several D. citri-associated viruses were discovered. The insect gut plays an important role as not only a pool for diverse microbes but also as a physical barrier to prevent the spread of pathogens such as CLas. However, there is little evidence of the presence of D. citri-associated viruses in the gut and of the interaction between them and CLas. Here, we dissected psyllid guts collected from five growing regions in Florida, and the gut virome was analyzed by high throughput sequencing. Four insect viruses, including D. citri-associated C virus (DcACV), D. citri densovirus (DcDV), D. citri reovirus (DcRV), and D. citri flavi-like virus (DcFLV), were identified, and their presence in the gut, including an additional D. citri cimodo-like virus (DcCLV), were confirmed with PCR-based assays. Microscopic analysis showed that DcFLV infection leads to morphological abnormalities in the nuclear structure in the infected psyllid gut cells. The complex and diverse composition of microbiota in the psyllid gut suggests a possible interaction and dynamics between CLas and the D. citri-associated viruses. Our study identified various D. citri-associated viruses that localized in the psyllid gut and provided more information that helps to evaluate the potential vectors for manipulating CLas in the psyllid gut.

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Biological control of citrus pests: A systematic bibliometric analysis 2000–2023

El Ammari,

Ziri,

El Bahja

et al. 2025

Journal of Agriculture and Food Research

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Diaphorina citri flavi-like virus localization, transmission, and association with Candidatus Liberibacter asiaticus in its psyllid host

Cited by 6 publications

References 50 publications

Screen and Character a New Iflavirus Virus in the Fruit Tree Pest Pyrops candelaria

Screen and Character a New Iflavirus Virus in the Fruit Tree Pest Pyrops candelaria

Identifying the Gut Virome of Diaphorina citri from Florida Groves

Biological control of citrus pests: A systematic bibliometric analysis 2000–2023

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