Diarrhea Accompanies Intestinal Inflammation and Intestinal Mucosal Microbiota Dysbiosis during Fatigue Combined with a High-Fat Diet

Abstract

Objective: It was reported fatigue or a high-fat diet triggers gastrointestinal (GI) disorders, and intestinal microbiota may play central roles in GI disorders. Therefore, we investigated the association between the intestinal mucosal microbiota and the intestinal mucosal barrier from fatigue combined with a high-fat diet. Method: This study divided the Specific pathogen-free (SPF) male into the normal group (MCN) and the standing united lard group (MSLD). After establishing the model for 14 days, interleukin-6 (IL-6), interleukin-17 (IL-17), immunoglobulin A (sIgA), mucin 2 (Muc2), and intestinal mucosal microbiota were analyzed. Furthermore, the correlations between bacterial genera, inflammation, and immune function were measured. Result: The pathological analysis proved obvious damage to the small intestinal structure after fatigue combined with a high-fat diet. MSLD mice presented an increased trend of IL-6 and IL-17 and a decreased tendency of sIgA and Muc2, aggravating the injury of the intestinal mucus barrier and GI symptoms. Fatigue combined with a high-fat diet considerably decreased Limosilactobacillus reuteri, positively associated with Muc2 and negatively with IL-6. Conclusion: The interactions between Limosilactobacillus reuteriand intestinal inflammation might be involved in the process of intestinal mucosal barrier impairment in fatigue combined with high-fat diet-induced diarrhea.