Diarrhoeagenic Escherichia coli and salmonellae in calves and lambs in Kashmir: absence, prevalence and antibiogram

Sa, Wani; Hussain, I.; Sa, Beg; Ma, Rather; Za, Kabli; Ma, Minghong; Nishikawa, Yoshikazu

doi:10.20506/rst.32.2.2213

Cited by 27 publications

(21 citation statements)

References 22 publications

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“…Newport was identified as the predominant serovar in our study regardless of the sample type, whereas S. Typhimurium and S. Kentucky were isolated at lower rates. This finding is in contrast to previous reports indicating S. Typhimurium to be either the predominant (Kidanemariam et al, 2010;Molla et al, 2006;Moussa et al, 2012;Stipetic et al, 2016;Tadesse, 2015;Wani et al, 2013;Yang et al, 2014) or the second-most dominant (Lewerin et al, 2011;Tadesse and Tessema, 2014) serovar isolated. There are only a few reports that mention S. Newport as the second-most dominant serovar and one of the lesser isolated serovars in sheep FC (Dargatz et al, 2015;Stipetic et al, 2016;USDA, 2013).…”

Section: Discussioncontrasting

confidence: 99%

Nontyphoid Salmonella Prevalence, Serovar Distribution and Antimicrobial Resistance in Slaughter Sheep

Çetin

Temelli

Eyigör

2020

Food Sci Anim Resour

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This study aimed to determine the current prevalence, serovar distribution and antimicrobial resistance rate and patterns of nontyphoid Salmonella (NTS) in slaughter sheep and their edible offal. While filling the gap of up to date related information in Turkey, data presented is also of significance since contamination of ovine meat, its products and offal with this pathogen is threat to public health due to their considerably high consumption rates in our country. Current NTS carriage in 200 apparently healthy slaughter sheep by ISO 6579:2002, 6579:2002/A1:2007 standard bacteriology (ISO) was 5% (10/200) (4 fecal content -2%, 3 mesenterial lymph node -1.5%, 3 kidney -1.5%) out of 1,400 samples (0.7%), with no isolation from carcass, liver, gallbladder, spleen. Realtime PCR was in substantial agreement to ISO in confirming Salmonella-suspect isolates (Relative Trueness: 93.6%). S. Newport (40%) was the predominant serovar, followed by the second prevalent serovars as S. Typhimurium and S. Kentucky (20%), and by S. Umbilo and S. Corvallis (10%). Four and 6 out of 10 NTS isolates were susceptible (40%) and resistant (60%) to 18 antimicrobials, respectively. S. Typhimurium isolates were multidrug resistant (MDR) to tigecycline and sulphamethoxazole/trimethoprim, with one also resistant to cefepime. S. Corvallis was MDR to ampicillin, ciprofloxacin, norfloxacin and pefloxacin. The predominance of S. Newport and first isolation of S. Corvallis in sheep in the world; first time isolations of Newport, Kentucky, Corvallis, Umbilo serovars from sheep in Turkey; and high antimicrobial resistance rates obtained in majority of the isolates highlights study findings.

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Section: Discussioncontrasting

confidence: 99%

Nontyphoid Salmonella Prevalence, Serovar Distribution and Antimicrobial Resistance in Slaughter Sheep

Çetin

Temelli

Eyigör

2020

Food Sci Anim Resour

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“…In fact, in the present study, amikacin was also the antibiotic that showed lower resistance rate among E. coli isolates from cattle, as well as observed in several studies in which it was included ( Fig. 5a) (Güler et al 2008;Scaria et al 2010;Wani et al 2013).…”

Section: Discussionsupporting

confidence: 85%

Antimicrobial susceptibility and phylotyping profile of pathogenic Escherichia coli and Salmonella enterica isolates from calves and pigs in Minas Gerais, Brazil

Souto

Coura

Dorneles

et al. 2016

Trop Anim Health Prod

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The aims of the present study were to determine (i) the profiles of phylogroup and (ii) the antimicrobial susceptibility of pathogenic Escherichia coli strains isolated from calves, and of Salmonella spp. strains isolated from calves and pigs in Minas Gerais State, Brazil. Sixty-one pathogenic E. coli strains and Salmonella spp. (n = 24) strains isolated from fecal samples of calves and Salmonella spp. (n = 39) strains previously isolated from fecal samples of growing/finishing pigs were tested. The minimum inhibitory concentration (MIC) using the agar dilution method was determined for nalidixic acid, amikacin, amoxicillin, ampicillin, cefoxitin, norfloxacin, gentamicin, tetracycline, and trimethoprim-sulfamethoxazole. All E. coli isolates were susceptible to amikacin. Tetracycline was the antimicrobial that presented the higher frequency of resistance among E. coli strains, followed by ampicillin, trimethoprim-sulfamethoxazole, amoxicillin, nalidixic acid, norfloxacin, gentamicin, and cefoxitin. E. coli (n = 61) strains isolated from calves belonged to different phylogroup namely, phylogroup A (n = 26), phylogroup B1 (n = 31), phylogroup E (n = 3), and phylogroup F (n = 1). Phylogroups B2, C, and D were not identified among the E. coli in the present study. All Salmonella spp. (n = 24) strains isolated from fecal samples of calves were susceptible to amikacin, amoxicillin, ampicillin, norfloxacin, gentamicin, tetracycline, and trimethoprim-sulfamethoxazole. Resistance to nalidixic acid and cefoxitin was detected in 16.66 and 8.33 % of the Salmonella spp. strains, respectively. Among the Salmonella spp. (n = 39) strains isolated from fecal samples of pigs, the higher frequency of resistance was observed to tetracycline, followed by amoxicillin, gentamicin, ampicillin, trimethoprim-sulfamethoxazole, nalidixic acid, cefoxitin, and norfloxacin. All strains were susceptible to amikacin. Forty-eight (78.68 %) of the E. coli strains were classified as multidrug-resistant, whereas among Salmonella spp. strains, the percentage of multidrug resistance was 57.14 %, being all multidrug-resistant strains isolated from pigs (92.30 %). The results from the present study indicate a high frequency of antimicrobial resistance among pathogenic E. coli strains isolated from calves and Salmonella spp. strains isolated from pigs and a high rate of susceptibility to most antimicrobials tested among Salmonella spp. strains isolated from calves. Our study highlights the presence of multidrug-resistant strains of E. coli and Salmonella spp. isolated from food-producing animals in Minas Gerais, Brazil.

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“…A variety of microorganisms have been isolated from faecal samples from lambs with diarrhoea, which implicates these organisms in aetiology of the syndrome, especially when the respective organisms have been isolated in pure culture. These results are in accord with previous findings in the literature (Manser and Dalziel, 1985;Adesiyun et al, 2001;Alvseike and Skjerve, 2002;Greco et al, 2005;Edrington et al, 2009;Osman et al, 2013;Ozmen et al, 2006;Wani et al, 2013;Gencay, 2014;Paz e Silva et al, 2014). Isolation in mixed culture from many samples may further indicate a potential synergistic role of these organisms.…”

Section: Discussionsupporting

confidence: 92%

Dissemination of intestinal pathogens between lambs and puppies in sheep farms

Chatzopoulos

Sarrou

Vasileiou

et al. 2016

Small Ruminant Research

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a b s t r a c tObjectives of the present work were (i) to confirm pathogens implicated in cases of diarrhoea in newborn and young lambs in sheep farms in Greece and (ii) to investigate a possible relation in dissemination of pathogens between lambs and dogs present in the farm. Work was carried out in 22 sheep farms, with (i) flock size over 150 animals, (ii) presence of clinical signs of diarrhoea in lambs in the flock and (iii) close and continuous contact and movement of shepherd dogs within the animal shed of each farm. Faecal sample collection from lambs was performed within 48 h of onset of clinical signs and prior to administration of any antimicrobial or antiparasitic medication to lambs. Faecal samples were also collected from puppies in the farm. In total, samples were collected from 126 lambs and 58 puppies. Samples were processed by using established techniques for isolation of bacteria, detection of viruses and observation of protozoan oocycts. Escherichia coli isolates obtained during the study, were tested for antimicrobial resistance against a variety of antimicrobial agents. In total, 236 bacterial isolates were recovered from faecal samples of lambs and 165 isolates from faecal samples of puppies. E. coli was the most frequently isolated microorganism: 104 isolates from lambs and 109 isolates from puppies were recovered. Other bacteria isolated were Enterobacter spp., Proteus spp., Klebsiella spp., (lambs and puppies), Clostridium perfringens, Citrobacter freundi, Salmonella enterica subsp. diarizonae (only lambs) and Streptococcus spp. (only puppies). Group A Rotavirus was detected in samples from lambs (2.5%) and Parvovirus in samples from puppies (5%). Cryptosporidium spp. oocysts were observed in samples from lambs and puppies. This is the first report of isolation of S. enterica subsp. diarizonae and of detection of Rotavirus from lambs in Greece. Rates of E. coli isolates from puppies resistant to antimicrobial agents were, in general, smaller than respective rates in isolates from lambs. Two pairs of isolates from the same farm (one from a lamb and one from a puppy) with identical patterns of resistance to antimicrobial agents were detected, which provides some evidence in support of a hypothesis that members of each pair might possibly have been spread from one animal species to the other.

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Diarrhoeagenic Escherichia coli and salmonellae in calves and lambs in Kashmir: absence, prevalence and antibiogram

Cited by 27 publications

References 22 publications

Nontyphoid Salmonella Prevalence, Serovar Distribution and Antimicrobial Resistance in Slaughter Sheep

Nontyphoid Salmonella Prevalence, Serovar Distribution and Antimicrobial Resistance in Slaughter Sheep

Antimicrobial susceptibility and phylotyping profile of pathogenic Escherichia coli and Salmonella enterica isolates from calves and pigs in Minas Gerais, Brazil

Dissemination of intestinal pathogens between lambs and puppies in sheep farms

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