Diarylheptanoid 1-(4-hydroxyphenyl)-7-phenyl-(6E)-6-hepten-3-one enhances C2C12 myoblast differentiation by targeting membrane estrogen receptors and activates Akt-mTOR and p38 MAPK-NF-κB signaling axes

Tipbunjong, Chittipong; Khuituan, Pissared; Kitiyanant, Yindee; Suksamrarn, Apichart; Pholpramool, Chumpol

doi:10.1007/s11418-019-01322-7

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…In this respect, experiments on C2C12 cells treated with diarylheptanoid compounds (HPPH) provide very important information (Tipbunjong et al 2017(Tipbunjong et al , 2019. These molecules display growth-and differentiationpromoting effects on C2C12 cells that involve a membrane form of ERα receptor bound by a palmitoyl anchor, and they were abolished by 2-bromohexadecanoic acid, an inhibitor of palmitoylation (Tipbunjong et al 2019).…”

Section: Discussionmentioning

confidence: 99%

20-Hydroxyecdysone activates the protective arm of the RAAS via the MAS receptor

Lafont

Serova

Didry-Barca

et al. 2022

Journal of Molecular Endocrinology

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20-Hydroxyecdysone (20E) is a steroid hormone that plays a key role in insect development through nuclear ecdysteroid receptors (EcR/RXR complex) and at least one membrane GPCR receptor (DopEcR). It also displays numerous pharmacological effects in mammals, where its mechanism of action is still debated, involving either an unidentified GPCR or the estrogen ERβ receptor. The goal of this study was to better understand 20E mechanism of action in mammals. A mouse myoblast cell line (C2C12) and the gene expression of myostatin (a negative regulator of muscle growth) was used as a reporter system of anabolic activity. Experiments using protein-bound 20E established the involvement of a membrane receptor. 20E-like effects were also observed with angiotensin-(1-7), the endogenous ligand of Mas. Additionally, the effect on myostatin gene expression was abolished by Mas receptor knock-down using small interfering RNA (siRNA) or pharmacological inhibitors. 17β-Estradiol (E2) also inhibited myostatin gene expression, but protein-bound E2 was inactive, and E2 activity was not abolished by angiotensin-(1-7) antagonists. A mechanism involving cooperation between the Mas receptor and a membrane-bound palmitoylated estrogen receptor is proposed. The possibility to activate the Mas receptor with a safe steroid molecule is consistent with the pleiotropic pharmacological effects of ecdysteroids in mammals and, indeed, the proposed mechanism may explain the close similarity between angiotensin-(1-7)’s and 20E’s effects. Our findings open up many possible therapeutic developments involving stimulation of the protective arm of the renin-angiotensin-aldosterone system (RAAS) with 20E.

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Section: Discussionmentioning

confidence: 99%

20-Hydroxyecdysone activates the protective arm of the RAAS via the MAS receptor

Lafont

Serova

Didry-Barca

et al. 2022

Journal of Molecular Endocrinology

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“…Besides, JNK has been shown to play a crucial role in cell proliferation, and the balance of JNK signaling will determine whether the cells are committed to proliferation or programmed cell death [ 58 ]. However, under the extended differentiation condition, activation of NF- κ B activity has been reported as a positive regulator of myoblast differentiation by stimulating MHC and myogenin expressions [ 59 , 60 ]. BPA exposure may impair NF- κ B activity via ER-Akt signaling since BPA treatment has been reported to suppress Akt signaling in myoblast leading to suppression of myoblast differentiation [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, Akt has been shown to mediate the regulation of NF- κ B activity by inducing phosphorylation and subsequent degradation of inhibitor of κ B (I κ B) [ 61 ]. Moreover, inhibition of NF- κ B activity has also been reported to interfere with the expression of myogenic regulatory factors [ 60 ]. However, the relationship between NF- κ B, JNK, and P53 proteins in myoblast exposed to BPA in both proliferation and differentiation conditions needs further elucidation.…”

Section: Discussionmentioning

confidence: 99%

Bisphenol-A Abrogates Proliferation and Differentiation of C2C12 Mouse Myoblasts via Downregulation of Phospho-P65 NF-κB Signaling Pathway

Tipbunjong,

Thitiphatphuvanon,

Pholpramool

et al. 2024

Journal of Toxicology

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Previous studies showed that bisphenol-A (BPA), a monomer of polycarbonate plastic, is leached out and contaminated in foods and beverages. This study aimed to investigate the effects of BPA on the myogenesis of adult muscle stem cells. C2C12 myoblasts were treated with BPA in both proliferation and differentiation conditions. Cytotoxicity, cell proliferation and differentiation, antioxidant activity, apoptosis, myogenic regulatory factors (MRFs) gene expression, and mechanism of BPA on myogenesis were examined. C2C12 myoblasts exposed to 25–50 µM BPA showed abnormal morphology, expressing numerous and long cytoplasmic extensions. Cell proliferation was inhibited and was accumulated in subG1 and S phases of the cell cycle, subsequently leading to apoptosis confirmed by nuclear condensation and the expression of apoptosis markers, cleaved caspase-9 and caspase-3. In addition, the activity of antioxidant enzymes, catalase, superoxide dismutase, and glutathione peroxidase was significantly decreased. Meanwhile, BPA suppressed myoblast differentiation by decreasing the number and size of multinucleated myotubes via the modulation of MRF gene expression. Moreover, BPA significantly inhibited the phosphorylation of P65 NF-κB in both proliferation and differentiation conditions. Altogether, the results revealed the adverse effects of BPA on myogenesis leading to abnormal growth and development via the inhibition of phospho-P65 NF-κB.

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Schizandrin attenuates inflammation induced by avian pathogenic Escherichia coli in chicken type II pneumocytes

Yuan

Peng

et al. 2020

International Immunopharmacology

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Diarylheptanoid 1-(4-hydroxyphenyl)-7-phenyl-(6E)-6-hepten-3-one enhances C2C12 myoblast differentiation by targeting membrane estrogen receptors and activates Akt-mTOR and p38 MAPK-NF-κB signaling axes

Cited by 4 publications

References 32 publications

20-Hydroxyecdysone activates the protective arm of the RAAS via the MAS receptor

20-Hydroxyecdysone activates the protective arm of the RAAS via the MAS receptor

Bisphenol-A Abrogates Proliferation and Differentiation of C2C12 Mouse Myoblasts via Downregulation of Phospho-P65 NF-κB Signaling Pathway

Schizandrin attenuates inflammation induced by avian pathogenic Escherichia coli in chicken type II pneumocytes

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