“…A total of five investigations were designed in this research. All the experiments were conducted at one factor a time, beginning with the introduction of different environmental factors such as incubator shaker agitation speeds (100, 130, and 160 rpm), light intensity (20, 27, and 34 µmol/m 2 /s), photoperiod (8,16, and 24 h) on the phototrophic cultivation of Amphora sp., followed by three main nutritional factors including glucose concentration (5,10,15,20, and 25 g/L), sucrose concentration (5, 10, 15, 20, and 25 g/L), and silicate concentration (50, 67, and 75 mg/L), the last on the heterotrophic cultivation of Amphora sp. Optimized variables would be brought forward to the subsequent experiments in all the above studies.…”