Diazepam fails to alter anxiety-like responses but affects motor function in a white-black test paradigm in larval zebrafish ( Danio rerio )

Zahid, Hifsa; Tsang, Benjamin; Ahmed, Hira; Lee, Richard Chi Yeung; Tran, Steven

doi:10.1016/j.pnpbp.2018.01.012

Cited by 15 publications

(9 citation statements)

References 57 publications

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“…Activity levels were also measured in larvae treated with tricaine (MS 222), a fish anesthetic, and diazepam (Valium), an anxiolytic and sedative, which is known to affect swim speed in zebrafish larvae 12,13 .…”

Section: Resultsmentioning

confidence: 99%

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Analysis of vertebrate vision in a 384-well imaging system

Thorn

Dombroski

Eller

et al. 2019

Sci Rep

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Visual impairment affects 253 million people worldwide and new approaches for prevention and treatment are urgently needed. While small molecules with potential beneficial effects can be examined in various model systems, the in vivo evaluation of visual function remains a challenge. The current study introduces a novel imaging system for measuring visually-guided behaviors in larval zebrafish. The imaging system is the first to image four 96-well plates with a single camera for automated measurements of activity in a 384-well format. In addition, it is the first system to project moving visual stimuli and analyze the optomotor response in the wells of a 96-well plate. We found that activity is affected by tricaine, diazepam and flumazenil. Surprisingly, diazepam treatments induce a loss of visual responses, at concentrations that do not affect activity or induce hyperactivity. Overall, our studies show that the developed imaging system is suitable for automated measurements of vertebrate vision in a high-throughput format.

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Section: Resultsmentioning

confidence: 99%

“…Diazepam is known to reduce swim speed in zebrafish larvae 12,13 . However, it is not known if diazepam can induce a complete loss of activity, which could be used as a model to study diazepam overdoses.…”

Section: Resultsmentioning

confidence: 99%

Analysis of vertebrate vision in a 384-well imaging system

Thorn

Dombroski

Eller

et al. 2019

Sci Rep

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“…Movement from one location to another is referred to as locomotor activity. Several behavioral and physiological functions are involved in locomotor activity and exploration (Zahid et al, 2018). Effects of various doses of pioglitazone on activities in open field and Fig.…”

Section: Discussionmentioning

confidence: 99%

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2021

Pak. J. Pharm. Sci

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Diabetics are twice as likely to have depression. It's normal to have long periods of sadness and anxiety. Pioglitazone has important role in the inflammatory response, which suggests that it might have the associated antidepressant effects being manifested by its anti-depressant profile which needs further exploration. Monitoring changes in behavioral and neurochemical profile of pioglitazone in a dose-dependent manner was the purpose of this study. Pioglitazone was injected to rats at the doses of 0mg/kg, 2.5mg/kg, 5mg/kg and 10mg/kg. Behavioral activities in open field, Skinner's box and elevated plus maze were monitored 20, 35 and 45 minutes respectively after pioglitazone injection. whole brain samples were collected following decapitation of rats one-hour after injection. Samples were kept at -70ºC till HPLC-EC analysis for neurochemical profile. Results show anxiogenic and sedative effects of pioglitazone at all three doses as indicated by Skinner's box, elevated plus maze activity and open field. Also there was an overall decreased dopamine metabolism and increased serotonin turnover. This suggests that diabetic patients using pioglitazone as a therapeutic option, may experience more potent effects of CNS depressants. Findings may help in extending therapeutics in diabetic patients suffering from anxiety and/or depression.

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“…A similar benzodiazepine, diazepam, administered to zebrafish embryos for 3 days, induced alterations on locomotion (1 nM) and on the genes involved in the circadian rhythm (both at 1 nM and 1 mM) (Oggier et al 2010). In addition, 6 dpf zebrafish exposed to higher concentrations (up to 2.5 mM) of diazepam had motor function impairments, but no alterations on anxiety-like responses (Zahid et al 2018). As the binding site for benzodiazepines in the brain is described to be highly conserved between fish and mammals (Carr and Chambers 2001), and as there are contradictory data regarding the use of these drugs during pregnancy (Neuman and Koren 2013), zebrafish seems to be a good model to clarify this issue, evaluating possible implications to humans and other animals.…”

Section: 4: Benzodiazepinesmentioning

confidence: 90%

Review on the use of zebrafish embryos to study the effects of anesthetics during early development

Félix

Coimbra

Valentim

et al. 2019

Critical Reviews in Toxicology

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Over the years, the potential toxicity of anesthetics has raised serious concerns about its safe use during pregnancy. As evidence emerged from research in animal models, showing that some anesthetic drugs are potential teratogenic, the determination of the risk of exposures to anesthetic drugs at early life stages became mandatory. However, due to inaccessibility and ethical constrains related to experimental conditions, the use of early life stages in mammalian models is limited. In this regard, some animal and nonanimal models have been suggested to surpass mammalian use in experimentation. Among them, the zebrafish embryo test has been recognized as a promising alternative in toxicology research, as well as an inexpensive and practical test. Substantial information collected from developmental research following compounds exposure, has contributed to the application of zebrafish assays in research, although only a few studies have focused on the use of early life stages of zebrafish to evaluate the developmental effects of anesthetics. Based on the recent advances of science and technology, there is a clear potential for zebrafish early life stages to provide new insights into anesthetics teratogenicity. This review provides an overview of recent anesthesia research using zebrafish embryos, demonstrating its usefulness to the anesthesia field, discussing the recent findings on various aspects related to the effects of anesthetics during early life development and the strengths and limitations of this model system.

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Diazepam fails to alter anxiety-like responses but affects motor function in a white-black test paradigm in larval zebrafish ( Danio rerio )

Cited by 15 publications

References 57 publications

Analysis of vertebrate vision in a 384-well imaging system

Analysis of vertebrate vision in a 384-well imaging system

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Review on the use of zebrafish embryos to study the effects of anesthetics during early development

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